TY  - JOUR
T1  - SIÇANLARDA SODYUM VALPROAT KAYNAKLI MİDE HASARI ÜZERİNE RUTİN’İN OKSİDATİF STRES, İNFLAMASYON VE APOPTOZ ÜZERİNDEKİ KORUYUCU ETKİLERİNİN ARAŞTIRILMASI
TT  - PROTECTIVE EFFECT OF RUTIN ON OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN VALPROAT-INDUCED GASTRIC TOXICITY
AU  - Tuncer, Sibel Çiğdem
AU  - Gür, Cihan
AU  - Akaras, Nurhan
AU  - Kandemir, Fatih Mehmet
PY  - 2023
DA  - September
Y2  - 2023
DO  - 10.17343/sdutfd.1251167
JF  - Medical Journal of Süleyman Demirel University
JO  - Med J SDU
PB  - Süleyman Demirel University
WT  - DergiPark
SN  - 1300-7416
SP  - 334
EP  - 342
VL  - 30
IS  - 3
LA  - tr
AB  - AmaçSodyum valproat, antiepileptik ilaçlardan en yaygınkullanılanlardan birisi olup uzun süreli maruziyet sonucundatoksik etkilidir. Uzun süreli sodyum valproatmaruziyeti dokularda özellikle oksidatif stres ve inflamasyonartışına neden olmaktadır. Rutin, birçok bitkidedoğal olarak bulunan antioksidan, antiinflamatuvarve antiapoptotik etkilere sahip bir flavanoiddir. Bu çalışmada,sodyum valproat kaynaklı mide doku hasarıüzerine doğal bir antioksidan olan rutinin kullanımı vemuhtemel etkilerinin araştırılması amaçlanmıştır.Gereç ve Yöntem35 adet Wistar albino cinsi sıçan kontrol, rutin, sodyumvalproat, sodyum valproat+rutin-50mg ve sodyumvalproat+rutin-100mg grupları olmak üzere 5gruba ayrıldı. 14 gün boyunca 500 mg/kg dozda sodyumvalproat uygulamasıyla birlikte 50 veya 100 mg/kg rutin uygulaması oral gavaj yolla yapıldı. 15. gündesıçanlar dekapite edilerek mide dokuları alındı. SOD,KAT, GPx aktiviteleri ile MDA, GSH seviyeleri ile oksidatifstres hasarı spektrofotormetrik yöntem ile analizedildi. NF-κB, TNF-α, COX-2 ve MMP-9 transkripsiyondüzeyleri ile inflamasyon hasarı ve Bax, Bcl-2,Kaspaz-3 mRNA transkripsiyon düzeyleri ile apoptotikhasar analizi RT-PCR yöntemi ile analiz edildi.Ayrıca konjesyon, hemoraji, mukoza hasarı, hücreinfiltrasyonu ve bez dilatasyonu açısından skorlamaiçin hematoksilen-eozin boyama ile histolojik analizleryapıldı.BulgularMide dokularında kontrol grubuna göre sodyumvalproat grubunda MDA düzeyi ile NF-κB, TNF-α,MMP-9, COX-2, Bax ve Kaspaz-3 mRNA transkripsiyondüzeyleri artmış (p
KW  - Apoptoz
KW  - İnflamasyon
KW  - Mide Toksisitesi
KW  - Oksidatif stres
KW  - Rutin
KW  - Valproat
N2  - ObjectiveSodium valproate is one of the most commonly usedantiepileptic drugs and is toxic after long-term exposure.Long-term exposure to sodium valproate causes anincrease in oxidative stress and inflammation in tissues.Rutin is a flavonoid naturally found in many plantswith antioxidant, anti-inflammatory, and antiapoptoticeffects. This study aimed to investigate the use andpossible outcomes of rutin, a natural antioxidant, ongastric tissue damage caused by sodium valproate.Material and Method35 Wistar albino rats were divided into 5 groups: control,rutin, sodium valproate, sodium valproate+rutin-50mg,and sodium valproate+rutin-100mg. For 14 days, a500 mg/kg dose of sodium valproate and 50 or 100mg/kg of rutin were administered by oral gavage. Onthe 15th day, rats were decapitated and gastric tissueswere removed. SOD, CAT, GPx activities, MDA, GSHlevels, and oxidative stress damage were analyzed byspectrophotometric method. Inflammation damage byNF-κB, TNF-α, COX-2, and MMP-9 transcription levelsand apoptotic damage by Bax, Bcl-2, and Caspase-3mRNA transcription levels were analyzed by RT-PCRmethod. Histologic analysis with hematoxylin-eosinstaining was also performed to score for congestion,hemorrhage, mucosal damage, cell infiltration andgland dilatation.ResultsIn gastric tissues, MDA level and NF-κB, TNF-α, COX-2, MMP-9, Bax and Caspase-3 mRNA transcriptionlevels increased (p
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UR  - https://doi.org/10.17343/sdutfd.1251167
L1  - https://dergipark.org.tr/en/download/article-file/2952662
ER  -