@article{article_1255946, title={The Expression Dynamics of Key Immune-Related Genes in Response to Mannheimia Haemolytica in Sheep Alveolar Macrophages In Vitro}, journal={Hayvan Bilimi ve Ürünleri Dergisi}, volume={6}, pages={1–13}, year={2023}, DOI={10.51970/jasp.1255946}, author={Çınar, Mehmet Ulaş and Al-janabı, Saif Adil Abbood and Sajid, Ghulam Asghar and Naji, Ahmed Qasim Naji and Sohel, Md Mahmodul Hasan}, keywords={Alveolar Macrophages, Immune related Genes, Gene Expression, M. haemolytica, Pneumonia}, abstract={Alveolar macrophages (AMs) respond to these infections as the first line of defense and trigger the lung’s immune response. Knowing the expression dynamics of key immune-related genes in alveolar macrophages against M. haemolytica challenge will help deepen the understanding of disease immunopathogenesis. For this purpose, first time an in vitro obtained from bronchoalveolar lavage (BAL) fluid and treated with three doses (T1: 1800, T2: 2700, T3: 5400 CFU) of M. haemolytica inoculum, while keeping one untreated as a control. Then, total RNA was extracted, and cDNA was synthesized. The real-time quantification data indicated that the expression of IL-1β, IL-6, IL-8, IL-10, and NF-κB genes were significantly changed as compared to control. Our study revealed that the exposure to M. haemolytica stimulates the immune response in the sheep alveolar macrophages in a dose and time-dependent manner.}, number={1}, publisher={Federation for Animal Science (FAS)}, organization={Erciyes University Scientific Research Projects Unit}