@article{article_1300194, title={Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites}, journal={Ankara Üniversitesi Veteriner Fakültesi Dergisi}, volume={71}, pages={417–426}, year={2024}, DOI={10.33988/auvfd.1300194}, author={Cevahir, Fatma and Düzlü, Önder and Atelge, Mübeccel and Yıldırım, Alparslan}, keywords={Cockroach, DNA barcoding, ITS-2, mt‐COI and mt‐COII, Vector}, abstract={This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica (n=105), Blatta orientalis (n=86), and Periplaneta americana (n=29). A total of 13 haplotypes were detected and maximum likelihood (ML) analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 (79.3%) belonged to helminth species and 12 (20.7%) to protozoan species. The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.}, number={4}, publisher={Ankara University}, organization={This study was supported by the Erciyes University Scientific Research Projects Unit with project number TDK-2017-7760.}