TY  - JOUR
T1  - Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites
AU  - Cevahir, Fatma
AU  - Düzlü, Önder
AU  - Atelge, Mübeccel
AU  - Yıldırım, Alparslan
PY  - 2024
DA  - September
DO  - 10.33988/auvfd.1300194
JF  - Ankara Üniversitesi Veteriner Fakültesi Dergisi
JO  - Ankara Univ Vet Fak Derg
PB  - Ankara University
WT  - DergiPark
SN  - 1300-0861
SP  - 417
EP  - 426
VL  - 71
IS  - 4
LA  - en
AB  - This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica (n=105), Blatta orientalis (n=86), and Periplaneta americana (n=29). A total of 13 haplotypes were detected and maximum likelihood (ML) analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 (79.3%) belonged to helminth species and 12 (20.7%) to protozoan species. The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.
KW  - Cockroach
KW  - DNA barcoding
KW  - ITS-2
KW  - mt‐COI and mt‐COII
KW  - Vector
CR  - Altay K, Dumanlı N (2015): Blattaria. 133-136. In: Karaer Z, Dumanlı N (Eds), Arthropodoloji. Medisan Press, Ankara.
CR  - Arakere G, Nadig S, Swedberg G, et al (2005): Genotyping of methicillin-resistant Staphylococcus aureus strains from two hospitals in Bangalore, South India. J Clin Microbiol, 43, 3198-3202.
CR  - Chamavit P, Sahaisook P, Niamnuy N (2011): The majority of cockroaches from the Samutprakarn province of Thailand are carriers of parasitic organisms. EXCLI Journal, 10, 218-222.
CR  - Chan OT, Lee EK, Hardman JM, et al (2004): The cockroach as a host for Trichinella and Enterobius vermicularis: implications for public health. Hawaii Med J, 63, 74-77.
CR  - Che Y, Gui S, Lo N, et al (2017): Species delimitation and phylogenetic relationships in ectobiid cockroaches (Dictyoptera, Blattodea) from China. PLoS One, 12, e0169006.
CR  - Che Y, Wang Z (2013): Three new species of cockroach genus Symploce Hebard, 1916 (Blattodea, Ectobiidae, Blattellinae) with redescriptions of two known species based on types from Mainland China. ZooKeys, 337, 1.
CR  - Cheng XF, Zhang LP, Yu DN, et al (2016): The complete mitochondrial genomes of four cockroaches (Insecta: Blattodea) and phylogenetic analyses within cockroaches. Gene, 586, 115-122.
CR  - Cetin H (2015): The importance of vector management for prevention of hospital infections. Turkiye Parazitol Derg, 39, 227-230.
CR  - El-Sherbini GT, El-Sherbini ET (2011): The role of cockroaches and flies in mechanical transmission of medical important parasites. J Entomol Nematol, 3, 98-104.
CR  - El-Sherbini GT, Gneidy MR (2012): Cockroaches and flies in mechanical transmission of medical important parasites in Khaldyia Village, El-Fayoum, Governorate, Egypt. J Egypt Soc Parasitol, 240, 1-10.
CR  - Evangelista D, Buss L, Ware JL (2013): Using DNA barcodes to confirm the presence of a new invasive cockroach pest in New York City. J Econ Entomol, 106, 2275-2279.
CR  - Evangelista DA, Bourne G, Ware JL (2014): Species richness estimates of B lattodea ss (Insecta: Dictyoptera) from northern G uyana vary depending upon methods of species delimitation. Syst Entomol, 39, 150-158.
CR  - Everaerts C, Maekawa K, Farine JP, et al (2008): The Cryptocercus punctulatus species complex (Dictyoptera: Cryptocercidae) in the eastern United States: comparison of cuticular hydrocarbons, chromosome number, and DNA sequences. Mol Phylogenet Evol, 47, 950-959.
CR  - Farmani M, Basseri H, Norouzi B, et al (2019): Ribosomal DNA internal transcribed spacer 2 sequence analysis and phylogenetic comparison of seven cockroach species in northwestern Iran. BMC Res Notes, 12, 1-5.
CR  - Fathpour H, Emtiazi G, Ghasemi E (2003): Cockroaches as reservoirs and vectors of drug resistant Salmonella spp. Iran Biomed J, 7, 35-38.
CR  - Fisk FW (1982): Abundance and diversity of arboreal Blattaria in moist tropical forests of the Panama canal area and Costa Rica. T Am Entomol, 479-489.
CR  - Folmer O, Black M, Hoeh W, et al (1994): DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol, 3, 294-299.
CR  - Galdiero E, Villari P, Onofrio V, et al (2005): Characterization of glycopeptide resistant enterococci isolated from a hospital in Naples (Italy). New Microbiol, 28, 171-176.
CR  - Gazeta GS, Freire ML, Ezequiel OS, et al (2007): Artrópodes capturados em ambiente hospitalar do Rio de Janeiro, Brasil. Rev Patol Trop, 36, 254-264.
CR  - Gliniewicz A, Sawicka B, Czajka E (2003): Occurrence of insect pests in hospitals in Poland. Przegl Epidemiol, 57, 329-334.
CR  - Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol, 52, 696-704.
CR  - Hamu H, Debalke S, Zemene E, et al (2014): Isolation of intestinal parasites of public health importance from cockroaches (Blattella germanica) in Jimma Town, Southwestern Ethiopia. J Parasitol Res, 186240, 1-5.
CR  - Hristov GH, Chobanov DP (2016). An annotated checklist and key to the Bulgarian cockroaches (Dictyoptera: Blattodea). Zootaxa, 4154, 351-388.
CR  - Hashemi-Aghdam SS, Rafie G, Akbari S, et al (2017): Utility of mtDNA-COI barcode region for phylogenetic relationship and diagnosis of five common pest cockroaches. J Arthropod-Borne Dis, 11, 182-193.
CR  - Huelsenbeck JP, Ronquist F (2001): MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754-755.
CR  - Jarujareet W, Kobayashi M, Taira K, et al (2019): The role of the American cockroach (Periplaneta americana) as transport host of Eimeria tenella to chickens. Parasitol Res, 118, 2311-2315.
CR  - Kearse M, Moir R, Wilson A, et al (2012): Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647-1649.
CR  - Kimura M (1980): A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol, 16, 111-120.
CR  - Knebelsberger T, Miller MA (2007): Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding). Zootaxa, 1522, 1-68.
CR  - Kutrup B (2003): Cockroach infestation in some hospitals in Trabzon, Turkey. Turk J Zool, 27, 73-77.
CR  - Lamiaa B, Lebbadi M, Ahmed A (2007): Bacteriological analysis of Periplaneta americana L. (Dictyoptera; Blattidae) and Musca domestica L. (Diptera; Muscidae) in ten districts of Tangier, Morocco. Afr J Biotechnol, 6, 2038-2042.
CR  - Librado P, Rozas J (2009): DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.
CR  - Miranda RA, Silva JP (2008): Enterobactérias isoladas de Periplaneta americana capturadas em um ambiente hospitalar. Ciência et Praxis, 1, 21-24.
CR  - Moges F, Eshetie S, Endris M, et al (2016): Cockroaches as a source of high bacterial pathogens with multidrug resistant strains in Gondar town, Ethiopia. Biomed Res Int, 1-6.
CR  - Mukha D, Wiegmann BM, Schal C (2002): Evolution and phylogenetic information content of the ribosomal DNA repeat unit in the Blattodea (Insecta). Ins Biochem Mol Biol, 32, 951-960.
CR  - Nei M, Kumar S (2000): Molecular evolution and phylogenetics: Oxford University Press, USA.
CR  - Oguz B, Ozdal N, Orunc Kilinc O, et al (2017): First investigation on vectorial potential of Blattella germanica in Turkey. Ankara Univ Vet Fak Derg, 64, 141-144.
CR  - Park YC, Maekawa K, Matsumoto T, et al (2004): Molecular phylogeny and biogeography of the Korean woodroaches Cryptocercus spp. Mol Phylogenet Evol, 30, 450-464.
CR  - Pongsiri MJ, Roman J (2007): Examining the links between biodiversity and human health: an interdisciplinary research initiative at the US Environmental Protection Agency. EcoHealth, 4, 82-85.
CR  - Posada D (2008): jModelTest: phylogenetic model averaging. Mol Biol Evol, 25, 1253-1256.
CR  - Salehzadeh A, Tavacol P, Mahjub H (2007): Bacterial, fungal and parasitic contamination of cockroaches in public hospitals of Hamadan, Iran. J Vect Borne Dis, 44, 105-110.
CR  - Sharawi ES, Mahyoub JA, Assagaf AI (2021): Morphological and molecular identification of the American cockroaches (Periplaneta americana)in Jeddah province (Dictyoptera: Blattidae). Int J Entomol Res, 6, 31-36.
CR  - Sulaiman IM, Anderson M, Khristova M, et al (2011): Development of a PCR–Restriction Fragment Length Polymorphism Protocol for Rapid Detection and Differentiation of Four Cockroach Vectors (Group I “Dirty 22” Species) Responsible for Food Contamination and Spreading of Foodborne Pathogens: Public Health Importance. J Food Prot, 74, 1883-1890.
CR  - Sulaiman IM, Jacobs E, Simpson S, et al (2017): Identification of 18 vector species belonging to Group I, Group II, and Group III ‘Dirty 22’species known to contaminate food and spread foodborne pathogens: DNA barcoding study of public health importance. Int J Trop Insect Sci, 37, 1-10.
CR  - Tachbele E, Erku W, Gebre-Michael T, et al (2006): Cockroach-associated food-borne bacterial pathogens from some hospitals and restaurants in Addis Ababa, Ethiopia: Distribution and antibiograms. JRTPH, 5, 34-41.
CR  - Tamura K, Stecher G, Peterson D, et al (2013): MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol, 30, 2725-2729.
CR  - Tatfeng YM, Usuanlele MU, Orukpe A, et al (2005): Mechanical transmission of pathogenic organisms: the role of cockroaches. J Vect Borne Dis, 42, 129-134.
CR  - Wang ZQ, Li Y, Che YL, et al (2015): The wood-feeding genus Cryptocercus (Blattodea: Cryptocercidae), with description of two new species based on female genitalia. Fla Entomol, 98, 260-271.
CR  - Yılmaz YB, Tunaz H (2013): Bazı bitki uçucu yağlarının ve monoterpenoid bileşenlerinin Amerikan hamambceği, Periplaneta americana (Dictyoptera: Blattidae), erginlerine karşı fumigant toksisitesi. Turk Entomol Derg, 37, 319-328.
CR  - Yue Q, Wu K, Qiu D, et al (2014): A formal re-description of the cockroach Hebardina concinna anchored on DNA barcodes confirms wing polymorphism and identifies morphological characters for field identification. PLoS One, 9, e106789.
CR  - Zheng Y, Wang C, Che Y, et al (2016): The species of Symplocodes Hebard (Blattodea: Ectobiidae: Blattellinae) with description of a new species from China. Ann Mag Nat Hist, 50, 339-361.
UR  - https://doi.org/10.33988/auvfd.1300194
L1  - https://dergipark.org.tr/en/download/article-file/3155927
ER  -