TY  - JOUR
T1  - The Used Masks of The Food Industry Employees During the Covid-19 Pandemic: Did the Mask Promote Other Diseases While Protecting from the Coronavirus? A Survey Study Supported by Microbiological Data
AU  - Dinçoğlu, Ahmet Hulusi
AU  - Özgür, Mustafa
AU  - Özgür, Elif Büşra
AU  - Çalışkan, Zühal
AU  - Rugji, Jerina
PY  - 2024
DA  - June
DO  - 10.33808/clinexphealthsci.1326608
JF  - Clinical and Experimental Health Sciences
PB  - Marmara University
WT  - DergiPark
SN  - 2459-1459
SP  - 431
EP  - 438
VL  - 14
IS  - 2
LA  - en
AB  - Objective: Use of disposable face masks helps prevent infection by airborne pathogens. The effectiveness of such masks in excluding diseases and contaminants depends on many factors. As a result of misuse, mask loses its protective role and becomes a source of microbial contamination. It is aimed to investigate the attitudes of food workers towards use of masks in proportion to the bacterial load and microorganism species in the masks they wear.Methods: Total aerobic mesophilic, Yeast-mould and Coliform counts were determined as log colony forming units per mL. Phenotypically different colonies grown on three different agar plates were purified and fresh cultures were classified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Results:In this study, bacterial contamination was found at different levels on all tested (103) disposable face masks. The screened bacterial pathogens by MALDI-TOF MS Bacillus cereus was detected at the highest level 17.86%, Enterobacter cloacae, Klebsiella oxytoca, Kurthia gibsonii and Pseudomonas aeruginosa with rates 14.29%, 10.72%, 7.14%, and 7.14%, respectively. Long-term working, inappropriate mask usage, poor hygiene attitudes of employees, and the fact that staff wore the mask out of need rather than for its protective advantage all signal that the investigated masks had low microbiological quality. Conclusion:The findings show that because most masks used by food industry employees to protect themselves from COVID-19 and avoid infecting others contain bacteria of intestinal origin, serious health problems may occur in both employees who use contaminated masks and consumers who consume food contaminated by mask contamination.
KW  - COVID-19
KW  - face masks
KW  - MALDI-TOF MS
KW  - food industry employees
KW  - microbiology
CR  - World Health Organization. Director-General’s opening remarks at the media briefing on COVID-19. https://www.who.int/director-general/speeches/detail/who-director-general-s-opening-remarks-at-the-media-brief-ing-on-Covid-19-11-March-2020
CR  - Nakat Z, Bou-Mitri C. COVID-19 and the food industry: Readiness assessment. Food Control. 2021; 121: 107661. DOI:10.1016/j.foodcont.2020.107661
CR  - Krebs C. Guidance on the essential critical infrastructure workforce: ensuring community and national resilience in COVID-19 response. https://pesquisa.bvsalud.org/global-literature-on-novel-coronavirus-2019- ncov/resource/pt/grc-740415
CR  - Galanakis CM. The food systems in the era of the coronavirus (Covid-19) pandemic crisis. Foods. 2020;9:523. DOI: 10.3390/foods9040523
CR  - Weersink A, von Massow M, McDougall B. Economic thoughts on the potential implications of Covid-19 on the Canadian dairy and poultry sectors. CJAE. 2020;68: 195–200. DOI: 10.1111/cjag.12240
CR  - Dinçoğlu AH, Rugji J. Covid-19 and food safety management systems. Türkiye Klinikleri J Health Sci. 2021;6:651–655. DOI: 10.5336/healthsci.2020-78224
CR  - CDC. Meat and poultry processing workers and employers. CDC 2020. https://www.cdc.gov/coronavirus/2019-ncov/community/organizations/meat-poultry-processing-workers-employers.html
CR  - Dyal JW, Grant MP, Broadwater K, Bjork A, Waltenburg MA, Gibbins JD, Hale C, Silver M, Fischer M, Steinberg J, Basler CA, Jacops JR, Kennedy ED, Tomasi S, Trout D, Hornsby-Myers J, Oussayef NL, Delaney LJ, Patel K, Shetty V, Kline KE, Schroeder B, Herlihy RK,  House J, Jervis R, Clayton JL, Ortbahn D, Austin C, Berl E, Moore Z, Buss BF, Stover D, Westergaard R, Pray I, DeBolt M, Person A, Gabel J, Kittle TS, Hendren P, Rhea C,  Holsinger C, Dunn J, Pinto G, Cooley LA, Saydah S, Preacely ND, Maddox RA, Lundeen E, Goodwin B, Karpathy SE, Griffing S, Jenkins MM, . Lowry G, Schwarz RD, Yoder J, Peacock G, Walke HT, Rose DA, Honein MA. Covid-19 among workers in meat and poultry processing facilities -19 States, April 2020. Morbidity and Mortality Weekly Report. 2022;69. DOI:10.15585/mmwr.mm6918e3
CR  - EFSA. Coronavirus: no evidence that food is a source or transmission route | EFSA. EFSA 2020. https://www.efsa.europa.eu/en/news/coronavirus-no-evidence-food-source-or-transmission-route.
CR  - IDFA. Proper usage of face masks/coverings to protect against COVID-19. IDFA 2020. https://www.cdc.gov/coronavirus/2019-ncov/prevent-getting-sick/cloth-face-cover.html.
CR  - WHO. COVID-19 and food safety: Guidance for food businesses: Interim Guidance. 2020. https://www.who.int/publications/i/item/covid-19-and-food-safety-guidance-for-food-businesses
CR  - OSHA. Guidance on preparing workplaces for COVID-19 2020.
CR  - WHO. Advice on the use of masks in the community, during home care and in health care settings in the context of the novel coronavirus (‎2019-nCoV)‎ outbreak: Interim guidance. WHO 2020. https://apps.who.int/iris/handle/10665/330987
CR  - Chughtai, A., Seale, H., MacIntyre, C. Use of cloth masks in the practice of infection control – evidence and policy gaps. International Journal of Infection Control 2013;9(3). DOI:10.3396/ijic.v9i3.11366
CR  - Luksamijarulkul P, Supapvanit C, Loosereewanich P, Aiumlaor P. Risk assessment towards tuberculosis among hospital personnel: Administrative control, risk exposure, use of protective barriers and microbial air quality. The Southeast Asian Journal of Tropical Medicine and Public Health 2004;35(4):1005-1011.
CR  - Delanghe L, Cauwenberghs E, Spacova I, de Boeck I, van Beeck W, Pepermans K, Claes I, Vandenheuvel D, Verhooven V, Lebeer S. Cotton and surgical face masks in community settings: Bacterial contamination and face mask hygiene. Front. Med. 2021;8:1477. DOI:10.3389/fmed.2021.732047
CR  - Zhang R, Li Y, Zhang AL, Wang Y, Molina MJ. Identifying airborne transmission as the dominant route for the spread of COVID-19. Proc. Natl. Acad. Sci. U.S.A. 2020;117:14857–14863.  DOI:10.1073/pnas.2009637117
CR  - Hendrix MJ, Walde C, Findley K, Trotman R. Absence of apparent transmission of SARS-CoV-2 from two stylists after exposure at a hair salon with a universal face covering policy. Morb. Mortal. Wkly. Rep. 2022;69:930–932.
CR  - Matuschek C, Moll F, Fangerau H, Fischer JC, Zänker K, van Griensven M, Schneider M, Kindgen‑Milles D, Trudo Knoefe W, Lichtenberg A, Tamaskovics B, Djiepmo Njanang FJ, Budach W, Corradini S, Häussinger D, Feldt T, Jensen B, Pelka R, Orth K, Peiper M, Grebe O, Maas K, Arne Gerber P, Pedoto A, Bölke E, Haussmann J. Face masks: Benefits and risks during the COVID-19 crisis. Eur. J. Med. Res. 2020;25:1–8. DOI:10.1186/s40001-020-00430-5
CR  - MacIntyre CR, Seale H, Dung TC, Hien NT, Nga PT, Chughtai AA, Rahman B, Dwyer D, Wang Q. A cluster randomised trial of cloth masks compared with medical masks in healthcare workers. BMJ 2015;5:006577. DOI:10.1136/bmjopen-2014-006577
CR  - Luksamijarulkul P, Aiempradit N, Vatanasomboon P. Microbial contamination on used surgical masks among hospital personnel and microbial air quality in their working wards: A hospital in Bangkok. Oman Med. J. 2014;29:346. DOI:10.5001/omj.2014.92
CR  - Fong P, Francis MJ, Hamblin JF, Korman TM, Graham M. Identification and diversity of actinomyces species in a clinical microbiology laboratory in the MALDI-TOF MS era. Anaerobe 2018;54:151–158. DOI:10.1016/j.anaerobe.2018.09.007
CR  - Boyce JM, Pittet D. Guideline for hand hygiene in health-care settings: Recommendations of the healthcare infection control practices advisory committee and the HICPAC/SHEA/APIC/IDSA hand hygiene task force. Morbidity and Mortality Weekly Report. 2002;51(RR16):1-45.
CR  - Türkiye Handwashing Survey Ministry of Health General Directorate of Health Promotion, 2012; Ankara/Türkiye
CR  - WHO. Advice on the use of masks in the context of COVID-19: interim guidance. 2020. https://reliefweb.int/report/world/advice-use-masks-context-covid-19-interim-guidance-5-june-2020?gclid=CjwKCAjw-7OlBhB8EiwAnoOEk7vKlA9vcmuHB3pSwK-MBnaPYY41lPtwCGXxySIEqI-FpcZbT4nqmRoCwMoQAvD_BwE
CR  - Sachdev R, Garg K, Singh G, Mehrotra V. Is safeguard compromised? Surgical mouth mask harboring hazardous microorganisms in dental practice. Fam. Med. Prim. Care Rev. 2020;9:759. DOI:10.4103/jfmpc.jfmpc_1039_19
CR  - Zhiqing L, Yongyun C, Wenxiang C, Mengning Y, Yuanqing M, Zhenan Z, Hasihan W, Jie Z, Kerong D, Huiwu L, Fengxiang L, Zanjing Z. Surgical masks as source of bacterial contamination during operative procedures. J. Orthop. Translat. 2018;14:57–62.  DOI:10.1016/j.jot.2018.06.002
CR  - Zheng Y, Chen H, Yao M, Li X. Bacterial pathogens were detected from human exhaled breath using a novel protocol. J Aerosol Sci. 2018;117:224–234.  DOI:10.1016/j.jaerosci.2017.12.009
CR  - Knibbs LD, Johnson GR, Kidd TJ, Cheney J, Grimwood K, Kattenbelt JA, O’Rourke PK, Ramsay K, Sly PD, Wainwright CE, Wood ME, Morawska L, Bell SC. Viability of Pseudomonas aeruginosa in cough aerosols generated by persons with cystic fibrosis. Thorax 2014;69:740–5. DOI:10.1136/thoraxjnl-2014-205213
CR  - Li J, Li M, Shen F, Zou Z, Yao M, Wu CY. Characterization of biological aerosol exposure risks from automobile air conditioning system. Environ. Sci. Technol. 2013;47:10660–10666.  DOI:10.1021/es402848d
CR  - Pyankov OV, Pyankova OG, Agranovski IE. Inactivation of airborne influenza virus in the ambient air. J Aerosol Sci. 2012;53:21–28. DOI:10.1016/j.jaerosci.2012.05.011
CR  - Ijaz MK, Zargar B, Wright KE, Rubino JR, Sattar SA. Generic aspects of the airborne spread of human pathogens indoors and emerging air decontamination technologies. Am. J. Infect. Control 2016;44:109–20. DOI:10.1016/j.ajic.2016.06.008
CR  - Gund MP, Boros G, Hannig M, Thieme-Ruffing S, Gärtner B, Rohrer TR, Simon A, Ruph S. Bacterial contamination of forehead skin and surgical mask in aerosol-producing dental treatment. J. Oral Microbiol. 2021;13(1):1978731. DOI:10.1080/20002297.2021.1978731
UR  - https://doi.org/10.33808/clinexphealthsci.1326608
L1  - https://dergipark.org.tr/en/download/article-file/3260734
ER  -