@article{article_1412809, title={Lawsonia inermis Linn. Positively Improves the Expression of Various Inflammatory Cytokines and Apoptotic Cell Death Biomarkers of Streptozotocin- Induced Diabetic Wistar Rats}, journal={Veterinary Sciences and Practices}, volume={18}, pages={123–132}, year={2023}, author={Aremu, Abdulfatai}, keywords={Lawsonia inermin Linn, inflammation, cytokines, interleukins, diabetes}, abstract={Diabetes mellitus is a significant contributor to illness and death on a global scale. Interleukins have been identified as potential factors that can induce apoptosis of beta cells and diminish insulin secretion, resulting in various complications associated with diabetes mellitus. This study investigated the inflammatory cytokines, interleukins, and apoptotic cell death markers in streptozotocin-induced diabetic rats. Lawsonia inermis leaves were sequentially extracted usingn-hexane, ethyl acetate, and methanol. Sixty-five male adult Wistar rats were grouped into 13, with 5 rats in each group. Streptozotocin-induced diabetic rats were treated as thus; 25, 50, and 100 mg kg–1 of each of the 3 partitioned extracts, metformin (500 mg kg–1), glibenclamide (5 mg kg–1), and untreated diabetic and nondiabetic rats were treated with distilled water for 28 days. Untreated diabetic rats showed an increased level of cytokines such as interleukin 1, interleukin 6, interleukin 12, and tumor necrosis factor alpha (TNF-α). The methanol fraction significantly decreased interleukin 1, interleukin 6, interleukin 12, interleukin 18, and TNF-α compared to other treatment groups and diabetic control. Methanol fraction of the extract showed a significant reduction in tTNF-α when compared to the 2 standard drugs and the controls. Nuclear factor-kappa beta increased nonsignificantly in both treated and untreated diabetic rats. There was a significant reduction in the expression of caspase-3, caspase-6, and caspase-9 in all the extract-treated groups. It was further noted that there is a slight increase level of B-cell lymphoma 2 in diabetic untreated rats. The study concluded that Lawsonia inermis Linn. has a significant positive modulatory effect on the expression of various cytokines, interleukins, and essential caspases that are implicated in the pathophysiology and pathogenesis of diabetes mellitus.}, number={3}, publisher={Ataturk University}