TY - JOUR T1 - Potential Role of Laurus nobilis Essential Oil in Reducing Indomethacin-Induced Gastric Ulcer in Rats TT - Laurus nobilis Esansiyel Yağının Sıçanlarda İndometasin Kaynaklı Mide Ülserinin Azaltılmasında Potansiyel Rolü AU - Üstündağ, Hilal AU - Taş, Necip Gökhan AU - Kurt, Nezahat AU - Issin, Gizem AU - Danışman Kalındemirtaş, Ferdane AU - Karakuş, Selcan PY - 2024 DA - March Y2 - 2024 DO - 10.26453/otjhs.1417755 JF - Online Turkish Journal of Health Sciences JO - OTSBD PB - Oğuz KARABAY WT - DergiPark SN - 2459-1467 SP - 89 EP - 96 VL - 9 IS - 1 LA - en AB - Objective: This study aimed to investigate the gastroprotective effects of Laurus nobilis leaves essential oil (LANO) against indomethacin (INDO)-induced gastric ulcers in rats.Materials and Methods: In this study, an indomethacin-induced gastric ulcer model was employed. 30 Sprague-Dawley rats were divided into five groups (n=6): Control, LANO, INDO, INDO with famotidine (FAM), and INDO with LANO. Indomethacin (25 mg/kg) induced ulcers, while LANO and FAM were administered by oral gavage at 200 mg/kg and 40 mg/kg, respectively. Gastric tissues underwent histopathological examination for ulceration, and biochemical assays measured total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and nitric oxide (NO) levels.Results: Compared to the INDO group, treatment with LANO significantly decreased the number of gastric ulcer foci. Biochemically, LANO moderated TOS and OSI levels and preserved TAS, indicating reduced oxidative stress. Additionally, LANO appeared to stabilize NO levels. These biochemical findings were corroborated by histopathological examination.Conclusions: The study's results indicate that LANO may be beneficial in protecting against NSAID-induced gastric damage. LANO's observed modulation of oxidative stress markers and NO levels suggests its potential role in managing gastric ulcers. KW - Gastric ulcer KW - indomethacin KW - Laurus nobilis KW - nitric oxide KW - oxidative stress N2 - Amaç: Bu çalışmanın amacı, sıçanlarda indometasin (INDO) ile indüklenen gastrik ülserlere karşı Laurus nobilis yaprakları uçucu yağının (LANO) gastroprotektif etkilerini araştırmaktır.Materyal ve Metot: Bu çalışmada, INDO ile indüklenen gastrik ülser modeli kullanıldı. 30 adet Sprague-Dawley sıçan beş gruba ayrıldı (n=6): Kontrol, LANO, INDO, famotidin (FAM) ile INDO, LANO ile INDO. INDO (25 mg/kg) ülserleri indüklerken, LANO ve FAM oral gavaj yoluyla sırasıyla 200 mg/kg ve 40 mg/kg olarak uygulandı. Gastrik dokular ülserasyon açısından histopatolojik incelemeye tabi tutuldu ve biyokimyasal analizlerle toplam oksidan durum (TOS), toplam antioksidan durum (TAS), oksidatif stres indeksi (OSI) ve nitrik oksit (NO) seviyeleri ölçüldü.Bulgular: İndometasin grubuyla karşılaştırıldığında, LANO ile tedavi gastrik ülser odaklarının sayısını önemli ölçüde azalttı. Biyokimyasal olarak, LANO uygulaması TOS ve OSI seviyelerini düşürdü ve TAS seviyesini korudu, bu da oksidatif stresin azaldığını göstermektedir. Ayrıca, LANO'nun NO seviyelerini stabilize ettiği görüldü. Bu biyokimyasal bulgular histopatolojik inceleme ile desteklendi.Sonuç: Çalışmanın sonuçları, LANO'nun INDO kaynaklı mide hasarına karşı korunmada faydalı olabileceğini göstermektedir. LANO'nun oksidatif stres belirteçleri ve NO seviyelerinde gözlenen modülasyonu, mide ülserlerinin yönetiminde potansiyel rolünü ortaya koymaktadır. CR - 1. Srivastav Y, Kumar V, Srivastava Y, Kumar M. Peptic Ulcer Disease (PUD), Diagnosis, and Current Medication-Based Management Options: Schematic Overview. Journal of Advances in Medical and Pharmaceutical Sciences. 2023;25(11), 14-27. doi:10.9734/jamps/2023/v25i11651 CR - 2. Malfertheiner P, Schulz C. Peptic ulcer: chapter closed?. Digestive Diseases. 2020;38(2):112-116. doi: 10.1080/10520295.2023.2221040 CR - 3. Duysak L, Gülaboğlu M, Yüksel TN, Halıcı Z, Canayakın D, Demir GM. Protective Effects of Ethanol Extract from Hippophae rhamnoides L. Leaves against Indomethacin-Induced Gastric Ulcer in Rats. Black Sea Journal of Health Science. 2023;6(4), 697-704. doi: 10.19127/bshealthscience.1336015 CR - 4. Paparella A, Nawade B, Shaltiel-Harpaz L, Ibdah M. A review of the botany, volatile composition, biochemical and molecular aspects, and traditional uses of Laurus nobilis. Plants. 2022;11(9):1209. doi:10.3390/plants11091209 CR - 5. Mohammed RR, Omer AK, Yener Z, Uyar A, Ahmed AK. Biomedical effects of Laurus nobilis L. leaf extract on vital organs in streptozotocin-induced diabetic rats: Experimental research. Annals of Medicine and Surgery. 2021;61:188-197. doi:10.1016/j.amsu.2020.11.051 CR - 6. Belasli A, Ben Miri Y, Aboudaou M, et al. Antifungal, antitoxigenic, and antioxidant activities of the essential oil from laurel (Laurus nobilis L.): Potential use as wheat preservative. Food Science & Nutrition. 2020;8(9):4717-4729. doi:10.1002/fsn3.1650 CR - 7. Al-Omran SA, Chinnikrishnaiah V, Ganesh VS, et al. Investigation of in vitro and in vivo Pursuits of Laurus nobilis Extract on Acetic Acid-induced Ulcerative Colitis in Wistar Rats. 2023;57(3s):644-651. doi:10.5530/ijper.57.3s.73 CR - 8. Koçer O, Türkmen M, Eren Y. Osmaniye Yöresinde Doğal Olarak Yetişen Defne (Laurus nobilis L.), Murt (Myrtus communis L.) Bitkilerinin Uçucu Yağ Oran ve Bileşenlerinin Belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(1):75-80. doi:10.24925/turjaf.v10i1.75-80.4974 CR - 9. Wong CP, Kobayashi Y, Yang J. Safety studies of LAURESH® a standardized Laurus nobilis leaf extract. Fundamental Toxicological Sciences. 2023;10(4):143-156. doi:10.2131/fts.10.143 CR - 10. Lee EH, Shin JH, Kim SS, Lee H, Yang SR, Seo SR. Laurus nobilis leaf extract controls inflammation by suppressing NLRP3 inflammasome activation. Journal of cellular physiology. 2019;234(5):6854-6864. doi:10.1002/jcp.27434 CR - 11. Toktay E, Yayla M, Sahin L, et al. The effects of dragon fruit (Hylocereus polyrhizus) extract on indomethacin‐induced gastric ulcer in rats. Journal of Food Biochemistry. 2022;46(9):e14274. doi:10.1111/jfbc.14274 CR - 12. Tometri SS, Ahmady M, Ariaii P, Soltani MS. Extraction and encapsulation of Laurus nobilis leaf extract with nano-liposome and its effect on oxidative, microbial, bacterial and sensory properties of minced beef. Journal of Food Measurement and Characterization. 2020;14:3333-3344. doi:10.1007/s11694-020-00578-y CR - 13. Natale G, Lazzeri G, Blandizzi C, et al. Seriate histomorphometry of whole rat gastric: an accurate and reliable method for quantitative analysis of mucosal damage. Toxicology and applied pharmacology. 2001;174(1):17-26. doi:10.1006/taap.2001.9193 CR - 14. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry. 2004;37(2):112-119. doi:10.1016/j.clinbiochem.2003.10.014 CR - 15. Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 2005;38(12):1103-1111. doi:10.1016/j.clinbiochem.2005.08.008 CR - 16. Ray A, Gulati K, Henke P. Stress gastric ulcers and cytoprotective strategies: perspectives and trends. Current Pharmaceutical Design. 2020. 26(25):2982-2990. CR - 17. Pineda-Peña EA, Chávez-Piña AE. Gaseous mediators (H2S, NO and CO) a new approach in gastrointestinal protection. Mexican Journal of Medical Research ICSA. 2023;11(21):39-47. doi:10.29057/mjmr.v11i21.9436 CR - 18. Tarnawski AS, Ahluwalia A. The critical role of growth factors in gastric ulcer healing: the cellular and molecular mechanisms and potential clinical implications. Cells. 2021;10(8):1964. doi:10.3390/cells10081964 CR - 19. Soundararajan L, Dharmarajan A, Samji P. Regulation of pleiotropic physiological roles of nitric oxide signalling. Cellular signalling. 2023;101:110496. doi:10.1016/j.cellsig.2022.110496 CR - 20. Lanas A. Role of nitric oxide in the gastrointestinal tract. Arthritis research & therapy. 2008;10(2):1-6. doi:10.1186/ar2465 CR - 21. Sánchez-Mendoza ME, López-Lorenzo Y, Cruz-Antonio L, et al. Gastroprotective effect of juanislamin on ethanol-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl groups in the mechanism of action. Molecules. 2020;25(9):2246. doi:10.3390/molecules25092246 CR - 22. Ulucan A, Etiopathogenesis of peptic ulcers and prostaglandin relationship. Van Medical Journal. 2020;27(2):238-245. doi:10.5505/vtd.2020.35744 CR - 23. Tarnawski AS, Ahluwalia A. Molecular mechanisms of epithelial regeneration and neovascularization during healing of gastric and esophageal ulcers. Current medicinal chemistry. 2012;19(1):16-27. doi:10.2174/092986712803414088 CR - 24. Liang TY, Deng RM, Li X, et al. The role of nitric oxide in peptic ulcer: a narrative review. Medical Gas Research. 2021;11(1):42. doi:10.4103/2045-9912.310059 CR - 25. Ugan RA, Un H. The protective roles of butein on indomethacin induced gastric ulcer in mice. The Eurasian Journal of Medicine. 2020;52(3):265. doi:10.5152/eurasianjmed.2020.20022 CR - 26. Stefanova G, Girova T, Gochev V, et al. Comparative study on the chemical composition of laurel (Laurus nobilis L.) leaves from Greece and Georgia and the antibacterial activity of their essential oil. Heliyon. 2020;6(12). doi:10.1016/j.heliyon.2020.e05491 CR - 27. Yazıcı M, Mazlum Y, Mehmet N, Çiğdem Ü, Türkmen M, Akaylı T. Effects of adding laurel (Laurus nobilis) essential oil to the diet of tilapia fish on growth and intestinal histology. Aquatic Sciences and Engineering. 2022;37(4):195-204. doi:10.26650/ASE20221101489 CR - 28. Pérez S, Taléns-Visconti R, Rius-Pérez S, Finamor I, Sastre J. Redox signaling in the gastrointestinal tract. Free Radical Biology and Medicine. 2017;104:75-103. doi:10.1016/j.freeradbiomed.2016.12.048 CR - 29. Al-Mijalli SH, Mrabti HN, Ouassou H, et al. Chemical Composition, Antioxidant, Anti-Diabetic, Anti-Acetylcholinesterase, Anti-Inflammatory, and Antimicrobial Properties of Arbutus unedo L. and Laurus nobilis L. Essential Oils. Life. 2022;12(11):1876. doi:10.3390/life12111876 CR - 30. Mssillou I, Agour A, El Ghouizi A, Hamamouch N, Lyoussi B, Derwich E. Chemical composition, antioxidant activity, and antifungal effects of essential oil from Laurus nobilis L. flowers growing in Morocco. Journal of Food Quality. 2020;1-8:8819311. doi:10.1155/2020/8819311 UR - https://doi.org/10.26453/otjhs.1417755 L1 - https://dergipark.org.tr/en/download/article-file/3652094 ER -