TY - JOUR T1 - A Rapid Method for In Vitro Plant Regeneration of Chia Salvia hispanica L. AU - M. Salıh, Shifa PY - 2025 DA - March Y2 - 2024 DO - 10.29133/yyutbd.1434550 JF - Yuzuncu Yıl University Journal of Agricultural Sciences JO - YYU J AGR SCI PB - Van Yuzuncu Yıl University WT - DergiPark SN - 1308-7576 SP - 1 EP - 8 VL - 35 IS - 1 LA - en AB - Chia Salvia hispanica L. is an annual herbaceous plant. The seeds were shown to have significant macronutrient and micronutrient components. Most of these common macronutrients are proteins, lipids with an appropriate fatty acid composition, ash, carbs, and dietary fiber. This study aimed to find a simple and fast protocol for the regeneration of this plant. The hypocotyls, stem, cotyledons, and young leaves explants of seedlings were grown on Murashige and Skoog (MS) medium fortified with various concentrations of (NAA) and 6-benzyl adenine (BA). Moreover, 2, 4-diclorophenoxyacetic acid (2, 4-D) and kinetin (Kin) were used. Callus was successfully induced from all explants. The most interesting result was the regeneration of shoots from young leaves in one-step during the callus formation. The appropriate medium for shooting was MS enriched with 0.5mg L-1 NAA and 1.0 mg L-1 BA as the percentage of shoot regeneration was (112.5 %). The regenerated shoots were rooted successfully in five days following transferring to agar solidified MS medium. These results revealed the effectiveness of the protocol used in the current study, which is considered the first that was able to produce the important medicinal plant S. hispanica in one-step during callus induction in a short period KW - Callus KW - Chia KW - Regeneration KW - Saliva hispanica CR - Adil, M., Ren, X., Kang, D. I., Thi, L. T., & Jeong, B. R. (2018).Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino. 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Journal of Applied Biology and Biotechnology, 7(1), 42-47. https://doi.org/10.7324/jabb.2019.70108 UR - https://doi.org/10.29133/yyutbd.1434550 L1 - https://dergipark.org.tr/en/download/article-file/3718470 ER -