TY  - JOUR
T1  - Chemotherapy-induced peripheral neuropathy in the neuronal cells model
TT  - Nöronal hücre modelinde kemoterapiye bağlı periferik nöropati
AU  - Ünay, Simge
PY  - 2024
DA  - September
Y2  - 2024
DO  - 10.17826/cumj.1439488
JF  - Cukurova Medical Journal
JO  - Cukurova Med J
PB  - Cukurova University
WT  - DergiPark
SN  - 2602-3032
SP  - 858
EP  - 859
VL  - 49
IS  - 3
LA  - en
AB  - This research aims to investigate the impacts of quercetin and low-level laser therapy (LLLT) on undifferentiated and nerve growth factor-differentiated PC12 cells experiencing cisplatin-induced peripheral neuropathy. PC12 cells treated with cisplatin were simultaneously exposed to quercetin and LLLT. We evaluated the effects of quercetin and LLLT on the expression of GAP-43 and Synapsin I genes through real-time PCR, cell viability using MTT assay, apoptosis induction via Annexin and dead assay, mitochondrial potential alterations using a mitopotential assay, and lactate dehydrogenase activity analysis in cells.  Our findings revealed that cisplatin led to increased apoptosis, mitochondrial dysfunction, and LDH activity, while reducing cell viability and inhibiting the expression of GAP-43 and Synapsin I genes in both undifferentiated and differentiated PC12 cells. However, the application of quercetin and LLLT resulted in decreased apoptosis, mitochondrial potential alterations, and lactate dehydrogenase activity. We propose further investigation into the in vivo effects of quercetin and low-level laser therapy on cisplatin-induced peripheral neuropathy, as well as exploring the molecular relationship between quercetin and low-level laser therapy.
KW  - Neuronal Cells
KW  - Cisplatin
KW  - Chemotherapy-induced peripheral neuropathy
N2  - Bu araştırma, quercetin ve düşük seviyeli lazer tedavisinin (LLLT) sisplatin kaynaklı periferik nöropati yaşayan farklılaşmamış ve sinir büyüme faktörü ile farklılaşmış PC12 hücreleri üzerindeki etkilerini araştırmayı amaçlamaktadır. Sisplatin ile muamele edilen PC12 hücreleri eş zamanlı olarak kuersetin ve LLLT'ye maruz bırakıldı. Kuersetin ve LLLT'nin GAP-43 ve Sinapsin I genlerinin ekspresyonu üzerindeki etkilerini gerçek zamanlı PCR, MTT testi ile hücre canlılığı, Annexin ve ölü testi ile apoptoz indüksiyonu, mitopotansiyel testi ile mitokondriyal potansiyel değişiklikleri ve hücrelerde laktat dehidrojenaz aktivite analizi ile değerlendirdik.  Bulgularımız sisplatinin hem farklılaşmamış hem de farklılaşmış PC12 hücrelerinde apoptoz, mitokondriyal disfonksiyon ve LDH aktivitesinde artışa yol açarken hücre canlılığını azalttığını ve GAP-43 ve Sinapsin I genlerinin ekspresyonunu inhibe ettiğini ortaya koymuştur. Bununla birlikte, kuersetin ve LLLT uygulaması apoptoz, mitokondriyal potansiyel değişiklikleri ve laktat dehidrojenaz aktivitesinde azalma ile sonuçlanmıştır. Quercetin ve düşük seviyeli lazer tedavisinin sisplatin kaynaklı periferik nöropati üzerindeki in vivo etkilerinin yanı sıra quercetin ve düşük seviyeli lazer tedavisi arasındaki moleküler ilişkinin araştırılmasını öneriyoruz.
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UR  - https://doi.org/10.17826/cumj.1439488
L1  - https://dergipark.org.tr/en/download/article-file/3739125
ER  -