@article{article_1440922, title={In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites}, journal={Aquatic Research}, volume={7}, pages={178–188}, year={2024}, DOI={10.3153/AR24016}, url={https://izlik.org/JA28XU65EK}, author={Taş, Beyhan and Kolören, Zeynep and Kolören, Onur}, keywords={Ceratophyllum demersum, Acanthamoeba castellanii, Amoebicidal activities, Hydrophyte}, abstract={Ceratophyllum demersum L. is a hydrophyte with potential for use as an analgesic, antipyretic and anti-inflammatory drug. It has also been stated that it is a hepatoprotective and anti-diarrheal agent with potential use in stomach disorders and respiratory diseases. Our study investigated the anti-amoebic activity of C. demersum, which became a hydrophyte underwater collected from Samsun River Miliç. Different concentrations of the pathogenic strain of Acanthamoeba castellanii (ATCC 30010) and the ethanolic extract of C. demersum were used to determine anti-Acanthamoeba activity. A. castellanii trophozoites were treated with C. demersum extract at different concentrations ranging from 1.9, 3.8, 7.6, 15.2, 30.4, 60.8 µg/mL and incubated at 26°C for 72 h. The 50% inhibitory concentration (IC50) of C. demersum extract was approximately 42.5 µg/mL at 72 hours. Trophozoite cell viability decreased to 42% and 58.33% in the presence of 30.4 and 60.8 µg/mL C. demersum extract at 72 hours, respectively. These results indicate that the ethanolic extract of C. demersum has anti-Acanthamoeba activity against A. castellanii trophozoites. The study highlights that this extract can be a potential protective drug source against Acanthamoeba trophozoites.}, number={4}