TY - JOUR T1 - In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites AU - Kolören, Zeynep AU - Taş, Beyhan AU - Kolören, Onur PY - 2024 DA - October Y2 - 2024 DO - 10.3153/AR24016 JF - Aquatic Research JO - Aquat Res PB - Nuray ERKAN ÖZDEN WT - DergiPark SN - 2618-6365 SP - 178 EP - 188 VL - 7 IS - 4 LA - en AB - Ceratophyllum demersum L. is a hydrophyte with potential for use as an analgesic, antipyretic and anti-inflammatory drug. It has also been stated that it is a hepatoprotective and anti-diarrheal agent with potential use in stomach disorders and respiratory diseases. Our study investigated the anti-amoebic activity of C. demersum, which became a hydrophyte underwater collected from Samsun River Miliç. Different concentrations of the pathogenic strain of Acanthamoeba castellanii (ATCC 30010) and the ethanolic extract of C. demersum were used to determine anti-Acanthamoeba activity. A. castellanii trophozoites were treated with C. demersum extract at different concentrations ranging from 1.9, 3.8, 7.6, 15.2, 30.4, 60.8 µg/mL and incubated at 26°C for 72 h. The 50% inhibitory concentration (IC50) of C. demersum extract was approximately 42.5 µg/mL at 72 hours. Trophozoite cell viability decreased to 42% and 58.33% in the presence of 30.4 and 60.8 µg/mL C. demersum extract at 72 hours, respectively. These results indicate that the ethanolic extract of C. demersum has anti-Acanthamoeba activity against A. castellanii trophozoites. The study highlights that this extract can be a potential protective drug source against Acanthamoeba trophozoites. KW - Ceratophyllum demersum KW - Acanthamoeba castellanii KW - Amoebicidal activities KW - Hydrophyte CR - Al-Halbosiy, M.M.F., Hassan, N.N., Jameel, S., AkramThabit, Z., Rasheed, K. A. (2020). Evaluation of phenolic extract from Ceratophyllum demersum against Leishmania sp., l20b cell line and bacteria. Annals of Tropical Medicine and Public Health, 23(10), 1–7. https://doi.org/10.36295/ASRO.2020.231030 CR - Ashok, D.T., Atul, M.K., Karale, S.S., Yashodhan, B.W. (2011). Evaluation of antidiarrhoeal and wound healing potentials of Ceratophyllum demersum Linn. whole plant in rats. Latin American Journal of Pharmacy, 30(2), 297–303. CR - Awati, S.S., Gilhotra, R.M., Singh, S.K., Raj, V., Wadkar, K.A. (2020). Plant profile, phytochemistry and pharmacological properties of Ceratophyllum demersum Linn.: A review. CR - Bhowmik, S., Datta, B. K., Saha, A. K. (2013). Ethno medicinal and phytochemical screening of some hydrophytes and marsh plants of Tripura, India. World Applied Sciences Journal, 22(10), 1453–1459. CR - Beulah, G.G., Soris, P.T., Mohan, V.R. (2018). GC-MS determination of bioactive compounds of Dendrophthoe falcata (LF) Ettingsh: an epiphytic plant. International Journal of Health Sciences and Research, 8, 261–269. CR - Borges, A, José, H, Homem, V, Simões, M. (2020). Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea. Antibiotics (Basel), 9(2), 48. https://doi.org/10.3390/antibiotics9020048 CR - Brunton, L.L., Lazo, J.S., Parker, K.L. (2006). Goodman’s & Gilman’s: The Pharmacological Basic of Therapeutics. McGraw-Hill companies, Medical Publishing Division. CR - Chiboub, O., Ktari, L., Sifaoui, I., López-Arencibia, A., Reyes-Batlle, M., Mejri, M., Valladares, B., Abderrabba, M., Piñero J.E., Lorenzo-Morales. J. (2017). In vitro amoebicidal and antioxidant activities of some Tunisian seaweeds. Experimental Parasitology, 183, 76–80. https://doi.org/10.1016/j.exppara.2017.10.012 CR - Degerli, S., Berk, S., Malatyali, E., Tepe, B. (2012a). Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch. & C.A. Mey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites. Parasitology Research,110(2), 565-70. https://doi.org/10.1007/s00436-011-2524-z CR - Degerli, S., Tepe, B., Celiksoz, A., Berk, S., Malatyali E. (2012b). In vitro amoebicidal activity of Origanum syriacum and Origanum laevigatum on Acanthamoeba castellanii cysts and trophozoites. Experimental Parasitology, 131(1), 20-24. https://doi.org/10.1016/j.exppara.2012.02.020 CR - Elsheikha, H.M., Siddiqui, R., Khan, N.A. (2020). Drug discovery against Acanthamoeba infections: Present knowledge and unmet needs. Pathogens, 9(5), 405. https://doi.org/10.3390/pathogens9050405 CR - Ertürk, Ö., Taş, B., Şahin, H. (2019). Antibacterial and antifungal activity of Eurasian water-milfoil collected from lentic and lotic water body in Central Black Sea Region, Turkey. Acta Biologica Turcica, 33(1), 12–19. CR - Fareed, M.F., Haroon, A.M., Rabeh, S.A. (2008). Antimicrobial activity of some macrophytes from Lake Manzalah (Egypt). Pakistan Journal of Biological Sciences, 11(21), 2454–2463. https://doi.org/10.3923/pjbs.2008.2454.2463 CR - Fareed, M.F., Haroon, A.M., Rabeh, S.A. (2008). Antimicrobial activity of some macrophytes from Lake Manzalah (Egypt). Pakistan Journal of Biological Sciences, 11(21), 2454–2463. https://doi.org/10.3923/pjbs.2008.2454.2463 CR - Gupta, A., Pandey, V.N. (2014). Herbal remedies of aquatic macrophytes of Gorakhpur district, Uttar Pradesh (India). International Journal of Pharma and Bio Sciences, 5(1), 300–308.Journal of Pharmaceutical Research & Education, 5(1), 409–422. CR - Islam, M.T., Ali, E.S., Uddin, S.J., Shaw, S., Islam, M.A, Ahmed, M.I, Atanasov, A.G. (2018). Phytol: a review of biomedical activities. Food and Chemical Toxicology, 121, 82–94. https://doi.org/10.1016/j.fct.2018.08.032 CR - Karale, S.S., Jadhav, S.A., Chougule, N.B., Awati, S.S., Patil, A.A. (2013). Evaluation of analgesic, antipyretic and antiinflammatory activities of Ceratophyllum demersum Linn. in albino rats. Current Pharma Research, 3(4), 1027–1030. https://doi.org/10.33786/JCPR.2013.v03i04.009 CR - Karatas, M., Dogan, M., Emsen, B., Aasim, M. (2015). Determination of in vitro free radical scavenging activities of various extracts from in vitro propagated Ceratophyllum demersum L. Fresenius Environmental Bulletin, 24, 2946–2952. CR - Kaynak, B., Koloren, Z., Karaman, U. (2018). Investigation of in vitro amoebicidal activities of Ornithogalum sigmoideum on Acanthamoeba castellanii cysts and trophozoites. Annals of Medical Research, 25(4), 709–715. https://doi.org/10.5455/annalsmedres.2018.07.145 CR - Kaynak, B., Koloren, Z., Karaman, U. (2019). Investigation of in vitro amoebicidal activities of Trachystemon orientalis on Acanthamoeba castellanii cysts and trophozoites. Van Medical Journal, 26(4), 483–490. https://doi.org/10.5505/vtd.2019.79926 CR - Khan, N.A. (2006). Acanthamoeba: Biology and increasing importance in human health. FEMS Microbiology Reviews, 30, 564-595. https://doi.org/10.1111/j.1574-6976.2006.00023.x CR - Koloren, O., Koloren, Z., Atli Şekeroğlu, Z., Çol Ayvaz, M., Karanis, P. (2019). Amoebicidal and amoebistatic effects of Artemisia argyi methanolic extracts on Acanthamoeba castellanii trophozoites and cysts. Acta Parasitologica, 64, 63–70. https://doi.org/10.2478/s11686-018-00009-5 CR - Kurashov, E.A., Fedorova, E.V., Krylova, J.V., Mitrukova, G.G. (2016). Assessment of the potential biological activity of low molecular weight metabolites of fresh water macrophytes with QSAR. Scientifica, 9, 1–9. https://doi.org/10.1155/2016/1205680 CR - Lass, A., Szostakowska, B., Idinska, A., Chomicz, L. (2014). The first genotype determination of Acanthamoeba potential threat to human health, isolated from natural water reservoirs in Poland. Parasitology Research, 113, 2693–2699. https://doi.org/10.1007/s00436-014-3925-6 CR - Li, Z., Tu, Z, Wang, H., Zhang, L. (2020). Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS. Molecules, 25(19), 4507. https://doi.org/10.3390/molecules25194507 CR - Li, Y., Zhao, X., Xia, M., Wei, X., Hou, H. (2023). Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake. Plant Diversity, https://doi.org/10.1016/j.pld.2023.08.002 CR - Lone, A.H., Balkhi, M.H., Magloo, A.H., Wanjari, R. N., Bazaz, A.I., Shah, M. A., Lone, H. Q. (2023). Antimicrobial activity of Ceratophyllum demersum against some fish pathogens in cold waters of Kashmir valley. The Pharma Innovation Journal. 12(10), 1155–1163. CR - Lu, X., Qiao, Y., Zhang, X., Ma, B., Qiu, M. (2007). Chemical constituents from Ceratophyllum demersum (Ceratophyllaceae). Aeta Bot. Yunnaniea, 29, 263–264. CR - Malathy, R., Shaleesha, A.S. (2015). Studies on the potential therapeutic effects on the aquatic macrophytes namely Cabomba aquatica, Ceratophyllum demersum and Hygrophila corymbose. Journal of Chemical and Pharmaceutical Research, 7(4), 479–483. CR - Malatyali, E., Tepe, B., Degerli, S., Berk, S., Akpulat, H.A. (2012a). In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites. Parasitology Research, 110(1), 167-74. https://doi.org/10.1007/s00436-011-2466-5 CR - Malatyali, E., Tepe, B., Degerli, S., Berk, S. (2012b). In vitro amoebicidal activities of Satureja cuneifolia and Melissa officinalis on Acanthamoeba castellanii cysts and trophozoites. Parasitology Research,110(6), 2175-2180. https://doi.org/10.1007/s00436-011-2744-2 CR - Monferran, M.V., Wunderlin, D.A., Nimptsch, J., Pflugmacher, S. (2007). Biotransformation and antioxidant response in Ceratophyllum demersum experimentally exposed to 1,2- and 1,4- dichlorobenzene. Chemosphere. 68(11), 2073–2079. https://doi.org/10.1016/j.chemosphere.2007.02.016 CR - Mujeeb, F., Bajpai, P., Pathak, N. (2014). Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of Aegle marmelos. BioMed Research International, 2014, 497606. https://doi.org/10.1155/2014/497606 CR - Niesink, R.J.M., Vries, J.D., Hollinger, M.A, (1996). Toxicology principles and applications.CRC Press, New York. 1312 p. CR - Nisha, R.P.B. (2018) Gas chromatography-mass spectrometry analysis for identifcation of bioactive compounds in selected genotypes of Trigonella foenum-graecum L. Pharma Innovation,7, 929–939. CR - Parmar, N., Desai, J. (1993). Review of the current methodology for the evaluation of gastric and duodenal anti-ulcer agents. Indian Journal of Pharmacology, 25, 120–135. CR - Ramya, R., Punitha, S.C., Aruna, G. (2021). Analysis of bioactive compounds from Stevia rebaudiana bertoni. Natural Volatiles and Essential Oils, pp 4560–4568. CR - Rashid, N., Ganiee, S.A., Bhat, M.S., Ganai, B.A. (2023). Comparative biochemical analysis and GC–MS phytochemical profiling in some aquatic plants. Chemical Papers, https://doi.org/10.1007/s11696-023-03217-0 CR - Roma, K., Kiran, S., Sahoo, D. (2017). Extraction and screening of bioactive compounds of some common hydrophytic and wetland plants from East Singbhum, Jharkhand, India. IOSR Journal of Pharmacy, 7(11), 23–29. CR - Taranhalli, A.D., Kadam, A.M., Karale, S.S., Warke, Y.B. (2011). Evaluation of antidiarrhoeal and wound healing potentials of Ceratophyllum demersum Linn. whole plant in rats. Latin American Journal of Pharmacy, 30(2), 297–303. Taş, B., Topaldemir, H. (2021). Assessment of aquatic plants in the Miliç Coastal Wetland (Terme, Samsun, Turkey). Review of Hydrobiology, 14(1-2), 1–23. CR - Tepe, B., Malatyalı, E., Değerli, S., Berk, S. (2012). In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts. Parasitology Research, 110(5), 1773–1778. https://doi.org/10.1007/s00436-011-2698-4 UR - https://doi.org/10.3153/AR24016 L1 - https://dergipark.org.tr/en/download/article-file/3745153 ER -