TY  - JOUR
T1  - Bioactivity-guided isolation and quantification of Chlorogenic acid from Calystegia silvatica (Kit.) Griseb. (Convolvulaceae)
TT  - Bioactivity-guided isolation and quantification of Chlorogenic acid from Calystegia silvatica (Kit.) Griseb. (Convolvulaceae)
AU  - Yüzbaşıoğlu Baran, Merve
AU  - Sener, Sıla Özlem
AU  - Kanbolat, Şeyda
AU  - Badem, Merve
AU  - Özgen, Ufuk
PY  - 2025
DA  - February
Y2  - 2024
DO  - 10.21448/ijsm.1451604
JF  - International Journal of Secondary Metabolite
JO  - Int. J. Sec. Metabolite
PB  - İzzet KARA
WT  - DergiPark
SN  - 2148-6905
SP  - 158
EP  - 165
VL  - 12
IS  - 1
LA  - en
AB  - This study aimed to investigate the bioactivity-guided isolation and quantification of chlorogenic acid from Calystegia silvatica, a plant traditionally utilized for its medicinal properties. The most cytotoxic aqueous sub-extract was subjected to open column chromatography to assess its cytotoxic activity against MCF-7 breast cancer cells using a resazurin reduction assay. The structure of the most active pure compound was determined to be chlorogenic acid (IC50= 36.44 ± 2.18 μg/mL) using 1D and 2D NMR spectroscopy. A validated high-performance liquid chromatography-diode array detector (HPLC-DAD) method was employed for the quantification of chlorogenic acid, which resulted in a content of 10.05 mg/g crude extract. The results indicate the potential of Calystegia silvatica as a source of chlorogenic acid with cytotoxic activity.
KW  - Chlorogenic acid
KW  - Resazurin assay
KW  - Calystegia silvatica
KW  - HPLC-DAD
KW  - Cytotoxic activity
N2  - This study aimed to investigate the bioactivity-guided isolation and quantification of chlorogenic acid from Calystegia silvatica, a plant traditionally utilized for its medicinal properties. The most cytotoxic aqueous sub-extract was subjected to open column chromatography to assess its cytotoxic activity against MCF-7 breast cancer cells using a resazurin reduction assay. The structure of the most active pure compound was determined to be chlorogenic acid (IC50= 36.44 ± 2.18 μg/mL) using 1D and 2D NMR spectroscopy. A validated high-performance liquid chromatography-diode array detector (HPLC-DAD) method was employed for the quantification of chlorogenic acid, which resulted in a content of 10.05 mg/g crude extract. The results indicate the potential of Calystegia silvatica as a source of chlorogenic acid with cytotoxic activity.
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UR  - https://doi.org/10.21448/ijsm.1451604
L1  - https://dergipark.org.tr/en/download/article-file/3789474
ER  -