TY - JOUR T1 - Investigation of cytogenetic, electrophoretic, histopathological and biochemical effects of walnut (juglans regia l.) Leaf extract in rats experimentally induced diabetes by streptozotocin AU - Asker, Hasan AU - Aksu Kılıçle, Pinar AU - Koç, Evren AU - Karadağ Sarı, Ebru AU - Doğan, Abdullah AU - Yıldız Asker, Yağmur PY - 2024 DA - August Y2 - 2024 DO - 10.31797/vetbio.1454981 JF - Journal of Advances in VetBio Science and Techniques JO - J Ad VetBio Sci Tech PB - İlker ÇAMKERTEN WT - DergiPark SN - 2548-1150 SP - 96 EP - 106 VL - 9 IS - 2 LA - en AB - In this study, the effects of Juglans regia L. (JR) leaf extract on histological damage and cytogenetic, electrophoretic, and biochemical parameters in the liver and kidney tissues of diabetic rats were investigated. In the study, 60 male rats (Sprague-Dawley) were separated into six groups. According to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) data, it was determined that there were increases and decreases in different serum protein expressions because of the treatment of JR leaf extract in the diabetes group. In the kidney tissue of the diabetes group, an increase in volume in the glomeruli and narrowing of the Bowman's space as well as thickening of the walls of tubules were detected. Vacuolization and shedding were observed in the epithelial cells of tubules in the cortical regions in kidney. In diabetic + JR extract groups administered JR leaf extract at doses of 250 mg/kg and 500 mg/kg, serum AST and ALT levels were reduced compared to the diabetic group. Diabetic rats' livers showed spotted necrosis and fibrosis in the portal area, biliary tract proliferation, mild inflammation, increased vascularization, unicellular necrosis in hepatocytes, and sinusoidal dilatation. The JR leaf extract group did not exhibit these problems. JR leaf extract influences reducing hepatotoxic and oxidative damage due to diabetes and increasing the level of antioxidant enzymes. As a result, it was concluded that the application of JR leaf extract may have a protective effect against damage caused by diabetes. KW - Diabetes KW - Juglans regia L. KW - kidney KW - liver KW - walnut CR - Arora, S., Brits, E., Kaur, S., Kaur, K., Sohi, R. S., Kumar, S., & Verschaeve, L. (2005). Evaluation or genotoxicity of medicinal plant extracts by the comet and VITOTOX (R) tests. Journal of Environmental Pathology Toxicology and Oncology, 24(3), 193-200. https://doi.org/10.1615/JEnvPathToxOncol.v24.i3.50 CR - Asokan, S. M., Wang, T., Su, W. T., & Lin, W. T. (2019). Antidiabetic effects of a short peptide of potato protein hydrolysate in STZ-Induced diabetic mice. Nutrients, 11(4), 779. https://doi.org/10.3390/nu11040779 CR - Aydin, S., Gokce, Z., & Yilmaz, O. (2015). The effects of Juglans regia L. 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