TY  - JOUR
T1  - The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study
AU  - Alacam, Tayfun
AU  - Şeker, İlke Doğa
AU  - Akca, Gülçin
AU  - Yılmaz, Aysel
AU  - Takka, Sevgi
PY  - 2025
DA  - January
Y2  - 2024
DO  - 10.26650/eor.20241459780
JF  - European Oral Research
JO  - EOR
PB  - Istanbul University
WT  - DergiPark
SN  - 2630-6158
SP  - 33
EP  - 39
VL  - 59
IS  - 1
LA  - en
AB  - Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis (E. faecalis) in infected root canals.Materials and Methods: A nano emulsion containing 20% R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MIC/MBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20% R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MIC/MBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria (Colony Forming Units, CFU/mL) were counted. Statistical analyzes were performed based on the data.Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control (p<0.05), however, none of the test materials completely eliminated E. faecalis from root canals.Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.
KW  - Antimicrobial effect
KW  - calcium hydroxide
KW  - Enterococcus faecalis
KW  - nano emulsion
KW  - R-limonene
CR  - Sunde PT, Olsen I, Debelian GJ, Tronstad L. Microbiota of periapical lesions refractory to endodontic therapy. J Endod 2002;28:304-10. google scholar
CR  - Byström A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Dent Traumatol 1985;1:170-5. google scholar
CR  - Sinha DJ, Sinha AA. Natural medicaments in dentistry. Ayu 2014;35:113-8. google scholar
CR  - Tewari R, Kapoor B, Mishra S, Kumar A. Role of herbs in endodontics. Journal of Oral Research and Review 2016;8:95-9. google scholar
CR  - Vishnuvardhini S, Sivakumar A, Ravi V, Prasad A, Sivakumar J. Herbendodonticsphytotherapy in endodontics: A review. Biomed Pharmacol J 2018;11:1073-82. google scholar
CR  - Almadi EM, Almohaimede AA. Natural products in endodontics. Saudi Med J 2018;39:124-30. google scholar
CR  - Jantrawut P, Boonsermsukcharoen K, Thipnan K, Chaiwarit T, Hwang KM, Park ES. Enhancement of antibacterial activity of orange oil in pectin thin film by microemulsion. Nanomaterials (Basel) 2018;8:545. google scholar
CR  - Seker ID, Akca G, Alacam T. In vitro evaluation of antimicrobial effects of citrus limonum essential oil on some endodontic pathogens. Ann Med Res 2022;29:427-33. google scholar
CR  - Zahi MR, El Hattab M, Liang H, Yuan Q. Enhancing the antimicrobial activity of d-limonene nano emulsion with the inclusion of s-polylysine. Food Chem 2017;221:18-23. google scholar
CR  - Zhang Z, Vriesekoop F, Yuan Q, Liang H. Effects of nisin on the antimicrobial activity of d-limonene and its nano emulsion. Food Chem 2014;150:307-12. google scholar
CR  - Donsı F, Annunziata M, Sessa M, Ferrari G. Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT - Food Science and Technology 2011;44:1908-14. google scholar
CR  - Turner S, Love R, Lyons K. An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine. Int Endod J 2004;37:664-71. google scholar
CR  - Feng J, Wang R, Chen Z, Zhang S, Yuan S, Cao H, Jafari SM, Yang W. Formulation optimization of d-limonene-loaded nano emulsions as a natural and efficient biopesticide. Colloids Surf A Physicochem Eng Asp 2020;596:124746. google scholar
CR  - Maté J, Periago PM, Palop A. Combined effect of a nano emulsion of d-limonene and nisin on listeria monocytogenes growth and viability in culture media and foods. Food Sci Technol Int 2016;22:146-52. google scholar
CR  - Alaçam T, Yoldaş HO, Gülen O. Dentin penetration of 2 calcium hydroxide combinations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;86:469-72. google scholar
CR  - Sun J. D-limonene: Safety and clinical applications. Altern Med Rev 2007;12:259-64. google scholar
CR  - Chikhoune A, Hazzit M, Kerbouche L, Baaliouamer A, Aissat K. Tetraclinis articulata(vahl) masters essential oils: Chemical composition and biological activities. Journal of Essential Oil Research 2013;25:300-7. google scholar
CR  - Vuuren Sv, Viljoen AM. Antimicrobial activity of limonene enantiomers and 1, 8-cineole alone and in combination. Flavour Fragr J 2007;22:540-4. google scholar
CR  - Chemat S, Tomao V, Chemat F. Limonene as green solvent for extraction of natural products. In: Mohammad A, editor. Green solvents i: Properties and applications in chemistry. Dordrecht: Springer Netherlands; 2012. p. 175-86. google scholar
CR  - Uemura M, Hata G, Toda T, Weine FS. Effectiveness of eucalyptol and d-limonene as guttapercha solvents. J Endod 1997;23:739-41. google scholar
CR  - Li PH, Chiang BH. Process optimization and stability of d-limonene-in-water nano emulsions prepared by ultrasonic emulsification using response surface methodology. Ultrason Sonochem 2012;19:192-7. google scholar
CR  - Weiss J, Gaysinsky S, Davidson M, McClements J. Nanostructured encapsulation systems: Food antimicrobials. In: Barbosa-Cánovas G, Mortimer A, Lineback D, Spiess W, Buckle K, Colonna P, editors. Global issues in food science and technology. San Diego: Academic Press; 2009. p. 425-79. google scholar
CR  - Baker J, Hamouda T, Shih A, Myc A, inventors; University of Michigan, assignee. Nontoxic antimicrobial compositions and methods of use. USA patent US-6559189-B2. 2003. google scholar
CR  - Zahi MR, Liang H, Yuan Q. Improving the antimicrobial activity of d-limonene using a novel organogel-based nano emulsion. Food Control 2015;50:554-9. google scholar
CR  - Sonu KS, Mann B, Sharma R, Kumar R, Singh R. Physico-chemical and antimicrobial properties of d-limonene oil nano emulsion stabilized by whey protein-maltodextrin conjugates. J Food Sci Technol 2018;55:2749-57. google scholar
CR  - Wennberg A, 0rstavik D. Evaluation of alternatives to chloroform in endodontic practice. Dent Traumatol 1989;5:234-7. google scholar
CR  - 0rstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Dent Traumatol 1990;6:142-9. google scholar
CR  - Bodrumlu E, Er O, Kayaoglu G. Solubility of root canal sealers with different organic solvents. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:e67-9. google scholar
CR  - Rehman K, Khan FR, Aman N. Comparison of orange oil and chloroform as gutta- percha solvents in endodontic retreatment. J Contemp Dent Pract 2013;14:478-82. google scholar
CR  - Whitworth JM, Boursin EM. Dissolution of root canal sealer cements in volatile solvents. Int Endod J 2000;33:19-24. google scholar
UR  - https://doi.org/10.26650/eor.20241459780
L1  - https://dergipark.org.tr/en/download/article-file/3825722
ER  -