TY - JOUR T1 - Rhizogenesis in Shrub rose cultivated in vitro AU - Konopelko, Alla AU - Koldar, Larysa AU - Denysko, Iryna AU - Mazur, Yevhen PY - 2024 DA - October Y2 - 2024 DO - 10.23902/trkjnat.1464147 JF - Trakya University Journal of Natural Sciences JO - Trakya Univ J Nat Sci PB - Trakya University WT - DergiPark SN - 2528-9691 SP - 187 EP - 196 VL - 25 IS - 2 LA - en AB - The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mg/l α-naphthylacetic acid (α-NAA), the most effective was the medium with the content of α-NAA 0.5 mg/l, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0%. On this medium, the frequency of rhizogenesis averaged 61.2% for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream (66.0%) and cv. Sommerabend (67.0%) had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mg/l α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0%. KW - micropropagation KW - roses KW - phytohormones KW - nutrient medium KW - determination KW - rooting N2 - Çalı güllerinin üreme özelliklerinin incelenmesi ve eksplant kök oluşumunun hormonal kontrolünün kullanılan besleyici ortamın fitohormon konsantrasyonuna bağlı olduğunun tanımlanması önemlidir ve hem bilimsel hem de pratik açıdan üzerinde durulan konulardır. Bu çalışmada, büyüme düzenleyicileri içeren besin ortamı üzerinde in vitro olarak yetiştiri-len çalı gülü kültivarlarının (Gärtnerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend) eksplantlarında kök oluşumunun hormonal belirlenmesine yönelik deneme-lerin sonuçları sunulmuştur. 0,2-1,0 mg/l a-naftilasetik asit (a-NAA) ilavesiyle modifiye edilen besin ortamlarından en etkilisinin, 0,5 mg/l a-NAA içerikli, sukroz oranı %2’ye kadar düşürülmüş ortam olduğu tespit edilmiştir. Bu ortamda, çalışılan kültivarlar için kök oluşu-mu sıklığı ortalama %61,2 olarak belirlenmiştir. Kullanılan kültivarlardaki kök oluşumunun in vitro etkinliğindeki hormonal katkının kültivarların genotiplerine bağlı olduğu belirlen-miştir: Lavender Dream kültivarı (%66,0) ve Sommerabend kültivarı (%67,0) en yüksekkök oluşumu yeteneği sergilemişlerdir. Bir büyüme düzenleyicisi olan Humifield'in 0,5 mg/l α-NAA ile kombinasyon halinde kullanılması, incelenen gül kültvarlarında köklenme oranının %70,0-86,0'a kadar artmasına katkıda bulunmuştur. CR - 1. Afrin, S., Rahman, M., Khalekuzzaman, M., Hasan, M., Fahim, A. & Alam, M. 2022. Study on in vitro micropropagation of Rosa sp. Bangladesh Journal of Agriculture, 47(1): 66-74. https://doi.org/10.3329/bjagri.v47i1.60593 CR - 2. Al-Mayahi, A. 2021. The effect of humic acid (HA) and zinc oxide nanoparticles (ZnO-NPS) on in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Quntar. Plant Cell, Tissue and Organ Culture (PCTOC), 145: 445-456. CR - 3. Arnold, N., Binns, M., Cloutier, C., Barthakur, N. & Pellerin, R. 1995. 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Series: Rural Sciences, 117: 44-53. https://doi.org/10.32851/2226-0099.2021.117.7 UR - https://doi.org/10.23902/trkjnat.1464147 L1 - https://dergipark.org.tr/en/download/article-file/3843627 ER -