TY - JOUR T1 - Potential of leek (Allium ampeloprasum) waste for microalgae Chlorella vulgaris cultivation: A preliminary evaluation TT - Pırasa (Allium ampeloprasum) atığının mikroalg Chlorella vulgaris kültürü için potansiyeli: Ön değerlendirme AU - Karaaslan, Muhammet Ali AU - Benas, Koray AU - Çakal Arslan, Özlem PY - 2024 DA - December Y2 - 2024 DO - 10.12714/egejfas.41.4.09 JF - Ege Journal of Fisheries and Aquatic Sciences JO - EgeJFAS PB - Ege University WT - DergiPark SN - 2148-3140 SP - 316 EP - 320 VL - 41 IS - 4 LA - en AB - Leek is an economical and healthy plant species. It contains rich dietary fibers, amino acids, bioactive compounds that increase its antioxidant capacity and more than 20 different fatty acids. It is rich in potassium, iron and selenium and can be used as a valuable source for microalgae cultivation. For impotance of leek, this study investigated the biomass production of Chlorella vulgaris microalgae species with leek leaf waste. To obtain the leek extract to be used for the experiment, leek leaves were dried in an oven at 40 °C and crushed in a mortar and pestle and filtered. Leek leaves were first dissolved with 10 ml DMSO (Dimethylsulfoxide) to 0.1 g/L and diluted with distilled water to a final volume of 100 ml. Chlorella vulgaris was exposed to leek extract concentrations of 0.01, 0.025, 0.05, 0.1 and 1.0 g/L for 72 hours and BG-11 enrichment medium was used in the control group. According to the data obtained, when leek leaves were used in the cultivation of C. vulgaris microalgae, a very high increase of 160% was observed at a concentration of 0.05 g/L compared to BG-11 enrichment medium. However, in the group where leek leaves were used completely, 64% increase was observed compared to the control group. This study proved that C. vulgaris have significant potential for food industries and the biocompost of vegetables is a suitable medium for microalgae cultivation. This study has proven that the use of vegetable wastes is suitable for obtaining a culture with high biomass of C. vulgaris microalgae, which has been used intensively in different areas of the food industry, and that leek wastes in particular provide high biomass growth. Therefore, the lower concentration of leek served as the best medium to increase the growth and biomass of C. vulgaris. This study proves that costs can be reduced and sustainable effective culture techniques can be used in microalgae culture by using vegetable wastes such as leek waste, which provides high biomass growth even at low concentrations. KW - Allium ampeloprasum KW - phytoplankton KW - biomass KW - aquaculture KW - recycling KW - agricultural waste N2 - Pırasa ekonomik ve sağlıklı bir bitki türüdür. Zengin diyet lifleri, amino asitler, antioksidan kapasitesini artıran biyoaktif bileşikler ve 20'den fazla farklı yağ asidi içerir. Potasyum, demir ve selenyum açısından zengindir ve mikroalg yetiştiriciliğinde değerli bir kaynak olarak kullanılabilir. Bu çalışmada, pırasa yaprak atıkları ile Chlorella vulgaris mikroalg türlerinin biyokütle üretiminin araştırılması amaçlanmıştır. Deneyde kullanılacak pırasa ekstraktını elde etmek için pırasa yaprakları 40 °C'de bir fırında kurutuldu ve bir havan ve tokmakla ezilerek süzüldü. Pırasa yaprakları önce 10 ml Dimetilsülfoksit ile 0,1 g/L'ye kadar çözüldü ve damıtılmış su ile son hacim 100 ml'ye kadar seyreltildi. C. vulgaris 0.01, 0.025, 0.05, 0.1 ve 1.0 g/L pırasa ekstraktı konsantrasyonlarına 72 saat süreyle maruz bırakılmış ve kontrol grubunda BG-11 zenginleştirme ortamı kullanılmıştır. Elde edilen verilere göre, pırasa yaprakları C. vulgaris yetiştirilmesinde kullanıldığında, 0.05 g/L konsantrasyonda BG-11 zenginleştirme ortamına göre %160 gibi çok yüksek bir artış gözlenmiştir. Ancak pırasa yapraklarının tamamen kullanıldığı grupta kontrol grubuna göre %64 oranında artış gözlenmiştir. Bu çalışma, gıda endüstrisinde farklı alanlarda yoğun olarak kullanılmaya başlanan C. vulgaris türünün yoğun biyokütleye sahip kültür elde edilmesinde sebze atıklarının kullanılmasının uygun olduğunu ve özellikle pırasa atıklarının yüksek biyokütle artışını imkan sağladığını kanıtlamıştır. Bu çalışma düşük konsantarasyon da dahi yüksek biyokütle artışını sağlayan pırasa atığı gibi bitkisel atıkların mikro alg kültüründe kullanılarak maliyetlerin düşürülebildiğini ve sürdürülebilir etkili kültür tekniklerinin kullanılabilirliğini ispatlamaktadır. CR - Ahmad, M.T., Shariff, M., Md. Yusoff, F., Goh, Y.M., & Banerjee, S. (2020). Applications of microalga Chlorella vulgaris in aquaculture. Reviews in Aquaculture, 12(1), 328-346. https://doi.org/10.1111/raq.12320 CR - Ak, İ., Oğuz, M., Benas, K., & Göksan, T. (2013). Cost-effective production of Arthrospira (Spirulina) platensis. 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