TY  - JOUR
T1  - Removal of pharmaceutically active compounds from hospital wastewater by ozonation pretreatment
AU  - Ateş, Havva
AU  - Mansımlı, Mahammad
PY  - 2025
DA  - September
Y2  - 2024
DO  - 10.35208/ert.1511061
JF  - Environmental Research and Technology
JO  - ERT
PB  - Mehmet Sinan Bilgili
WT  - DergiPark
SN  - 2636-8498
SP  - 571
EP  - 580
VL  - 8
IS  - 3
LA  - en
AB  - Hospital wastewater includes many pharmaceutically active compounds (PhACs). Since this resulted in both PhACs distribution to the environment and development of antibiotic resistance in microorganisms, on-site treatment of hospital wastewater has gained importance. In this study, the removal of 21 PhACs consisting of 12 parent compounds and 9 main metabolites from hospital wastewater by ozonation was investigated. In this context, commonly used analgesics (Paracetamol, Diclofenac, Ibuprofen, and Naproxen, 4'-Hydroxydiclofenac, 5-Hydroxydiclofenac, 1-Hydroxyibuprofen, 2-Hydroxyibuprofen, Carboxyibuprofen, (S)-O-Desmethyl naproxen) and antibiotics (Ciprofloxacin, Sulfamethoxazole, Trimethoprim, Erythromycin, Metronidazole, Clarithromycin, Azithromycin, Clindamycin, N-Acetyl-Sulfamethoxazole, Sulfamethoxazole-β-D-Glucuronide, Clindamycin sulfoxide) were selected. PhAC analyses were performed by HPLC/MS-MS. The ozonation dose was between 0.05-5.0 mg O3/mg COD. In real hospital wastewater, many of the selected PhACs were detected and total analgesic and antibiotic were determined as 22.9 and 40.6 µg/L, respectively. The results showed that detected PhACs were completely removed at 1.5 mg O3/mg COD. Sulfamethoxazole was degraded at the lowest dose of ozone (0.05mg O3/mg COD), while Ciprofloxacin and 2-Hydroxy ibuprofen were relatively resistant to non-stoichiometric doses of ozone. The removal efficiencies of Ciprofloxacin and 2-Hydroxy ibuprofen were determined as 77% and 37%, respectively, at 0.5 mg O3/mg COD. Additionally, COD removal was 48% at 1.5 mg O3/mg COD. As a result, pre-oxidation of hospital wastewater can be an effective method for on-site pretreatment of PhACs.
KW  - Hospital wastewater
KW  - ozonation
KW  - antibiotics
KW  - analgesics
CR  - S. Hocaoglu, Celebi, M., Basturk, M. D. and R. Partal, “Treatment-based hospital wastewater characterization and fractionation of pollutants,” Journal of Water Process Engineering, Vol. 43, pp. 102205, 2012.
CR  - A. Majumder, A. K. Gupta, P. S., Ghosal, and M. Varma, “A review on hospital wastewater treatment: A special emphasis on occurrence and removal of pharmaceutically active compounds, resistant microorganisms, and SARS-CoV-2,” Journal of environmental chemical engineering, Vol. 9(2), pp. 10481, 2021.
CR  - L. Lien, N. Hoa, N. Chuc, N. Thoa, H. Phuc, V. Diwan, N. Dat, A. Tamhankar, C. Lundborg, “Antibiotics in wastewater of a rural and an urban hospital before and after wastewater treatment, and the relationship with antibiotic use—a oneyear study from Vietnam,” IJERPH, Vol. 13, pp. 588, 2016.
CR  - Y. Luo, W. Guo, H. H. Ngo, L. D. Nghiem, F. I. Hai, J. Zhang, S. Liang, and X. C., Wang, “A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment,” Science of The Total Environment, Vol. 473-474, pp. 619-641, 2014.
CR  - S. Dires, T. Birhanu, A. Ambelu, G. Sahilu, “Antibiotic resistant bacteria removal of subsurface flow constructed wetlands from hospital wastewater,” J. Environ. Chem. Eng. Vol. 6, pp. 4265–4272, 2018.
CR  - M.I. Pariente, Y. Segura, S. Alvarez-Torrellas, J.A. Casas, Z.M. dePedro, E. Diaz, J. Garcıá , M.J. Lopez-Munoz, J. Marugan, A.F. Mohedano, R. Molina, M. Munoz, C. Pablos, J.A. Perdigon-Melon, A.L. Petre, J.J. Rodrıǵuez, M. Tobajas, F. Martıńez, “Critical review of technologies for the on-site treatment of hospital wastewater: From conventional to combined advanced processes,” Journal of Environmental Management, Vol. 320, pp. 115769, 2022.
CR  - S. Aydin, M.E. Aydin, A. Ulvi, H. Kilic, “Antibiotics in hospital effluents: occurrence, contribution to urban wastewater, removal in a wastewater treatment plant, and environmental risk assessment,” Environmental Science and Pollution Research, Vol. 26, pp. 544–558, 2019.
CR  - B. Z. Gönder, M. E. Kara, O. B. Celik, I. Vergili, Y. Kaya, S. M. Altinkum, Y. Bagdatli, G. Yilmaz, “Detailed characterization, antibiotic resistance and seasonal variation of hospital wastewater,” Environmental Science and Pollution Research, Vol. 28, pp. 16380–16393, 2021.
CR  - G. Yilmaz, Y. Kaya, I. Vergili, B. Z. Gönder, G. Özhan, O. B.. Celik, M. S. Altinkum, Y. Bagdatli, A. Boergers, J. Tuerk, “Characterization and toxicity of hospital wastewaters in Turkey,” Environmental Monitoring and Assessment, Vol. 189, pp. 55, 2017.
CR  - S. Top, M. Akgün, E. Kıpçak and M. S. Bilgili, “Treatment of hospital wastewater by supercritical water oxidation process,” Water research, Vol. 185, pp. 116279, 2020.
CR  - G. K Akkaya, “Evaluation of electrochemical treatment of real hospital wastewater with different electrode materials,” Environmental Research and Technology, Vol. 3(4), 175-181.
CR  - E. İnce, M. İnce, H, Yaşar, and Y. A. Uslu, “Membrane fouling in thermophilic aerobic membrane distillation bioreactor treating hospital wastewater,” Water SA, Vol. 48(4), 467-475, 2022.
CR  - N. A. Khan, V. Vambol, S. Vambol, B. Bolibrukh, M. Sillanpaa, F. Changani,... and M. Yousefi, “Hospital effluent guidelines and legislation scenario around the globe: A critical review,” Journal of Environmental Chemical Engineering, 9(5), 105874, 2021.
CR  - A. Azar, A. Jelogir, G. Bidhendi, N. Mehrdadi, N. Zaredar, M. Poshtegal, “Investigation of optimal method for hospital wastewater treatment,” Journal of Food Agriculture and Environment, Vol. 8, pp. 1199–1202, 2010.
CR  - S. Yuan, X. Jiang, X. Xia, H. Zhang, S. Zheng, 2013, “Detection, occurrence and fate of 22 psychiatric pharmaceuticals in psychiatric hospital and municipal wastewater treatment plants in Beijing, China,” Chemosphere, Vol. 90, pp. 2520–2525, 2013.
CR  - M.E. Casas, R.K. Chhetri, G. Ooi, K.M.S. Hansen, K. Litty, M. Christensson, C. Kragelund, H.R. Andersen, K. Bester, “Biodegradation of pharmaceuticals in hospital wastewater by staged Moving Bed Biofilm Reactors (MBBR),” Water Research, Vol. 83, pp. 293–302, 2015.
CR  - G.T.H. Ooi, K. Tang, R.K. Chhetri, K.M.S. Kaarsholm, K. Sundmark, C. Kragelund, K. Litty, A. Christensen, S. Lindholst, C. Sund, M. Christensson, K. Bester, H. R. Andersen, “Biological removal of pharmaceuticals from hospital wastewater in a pilot-scale staged moving bed biofilm reactor (MBBR) utilising nitrifying and denitrifying processes,” Bioresource Technology, Vol. 267, pp. 677–687, 2018.
CR  - P. Ajo, S., Preis, T. Vornamo, M. Manttari, M. Kallioinen, M. Louhi-Kultanen, “Hospital wastewater treatment with pilot-scale pulsed corona discharge for removal of pharmaceutical residues,” Journal of Environmental Chemical Engineering, Vol. 6, pp. 1569–1577, 2018.
CR  - B. Gupta, A.K. Gupta, P.S. Ghosal, C.S. Tiwary, “Photo-induced degradation of biotoxic Ciprofloxacin using the porous 3D hybrid architecture of an atomically thin sulfur-doped g-C3N4/ZnO nanosheet,” Environmental Research, Vol.183, pp 109154, 2020.
CR  - V. Chitnis, S. Chitnis, K. Vaidya, S. Ravikant, S. Patil, D.S. Chitnis, “Bacterial population changes in hospital effluent treatment plant in central India,” Water Research, Vol. 38, pp. 441–447, 2004.
CR  - C. I. Kosma, D. A. Lambropouolu, T. A. Albanis, “Occurrence and removal of PPCPs in municipal and hospital wastewaters in Greece,” Journal of Hazardous Materials, Vol. 179, pp. 804-817, 2010.
CR  - L. Kovalova, H. Siegrist, U. von Gunten, J. Eugster, M. Hagenbuch, A. Wittmer, R. Moser, C.S. McArdell, “Elimination of micropollutants during post-treatment of hospital wastewater with powdered activated carbon, ozone, and UV,” Environmental Science&Technology, Vol. 47, pp. 7899e7908, 2013.
CR  - J. Zheng, C. Su, J. Zhou, L. Xu, Y. Qian, H Chen., “Effects and mechanisms of ultraviolet, chlorination, and ozone disinfection on antibiotic resistance genes in secondary effluents of municipal wastewater treatment plants,” Chemical Engineering Journal, Vol. 317, pp. 309-316, 2017.
CR  - Y. Xue, M. Kamali, B. Kakavandi, M. E. V. Costa, I. P. Thompson, W. Huang,... & R. Dewil, “Activation of peracetic acid by a magnetic biochar-ferrospinel AFe2O4 (A= Cu, Co, or Mn) nanocomposite for the degradation of carbamazepine− A comparative and mechanistic study,” Chemical Engineering Journal, Vol. 490, pp. 151932, 2024.
CR  - F. Hayati, M. R. Khodabakhshi, A. A. Isari, S. Moradi, and B. Kakavandi, “LED-assisted sonocatalysis of sulfathiazole and pharmaceutical wastewater using N, Fe co-doped TiO2@ SWCNT: optimization, performance and reaction mechanism studies,” Journal of Water Process Engineering, Vol. 38, pp. 101693, 2020.
CR  - U.V. Gunten, M.M. Huber, S. Canonica, G.Y. Park, “Oxidation of Pharmaceuticals during Ozonation and Advanced Oxidation Processes,” Environmental Science & Technology, Vol. 37(5), pp. 1016–10242003, 2003.
CR  - A.D. Coelho, C. Sans, A. Agüera, M.J. Gómez, S. Esplugas, M., Dezotti, “Effects of ozone pre-treatment on diclofenac: Intermediates, biodegradability and toxicity assessment. Science of the Total Environment,” Vol. 407, pp. 3572–3578, 2009.
CR  - Y. Lee, L. Kovalova, C.S. McArdell, U. Gunten, “Prediction of micropollutant elimination during ozonation of a hospital wastewater effluent,” Water research, Vol. 64, pp. 134-148 2014.
CR  - C. N. D. Thanh, V. T. K. Quyen, N. T. Tin and B. X. Thanh, “Performance of ozonation process as advanced treatment for antibiotics removal in membrane permeate,” GeoScience Engineering, Vol. 62(2), pp. 21, 2016.
CR  - K. M. Hansen, A. Spiliotopoulou, R. K. Chhetri, M. E. Casas, K. Bester, and H. R. Andersen, “Ozonation for source treatment of pharmaceuticals in hospital wastewater–Ozone lifetime and required ozone dose,” Chemical Engineering Journal, Vol. 290, pp. 507-514, 2016.
CR  - F. S. Souza, V. V. Da Silva, C. K. Rosin, L. Hainzenreder, A. Arenzon, T. Pizzolato, ... and L. A. Féris, “Determination of pharmaceutical compounds in hospital wastewater and their elimination by advanced oxidation processes,” Journal of environmental science and health, part A, Vol. 53(3), pp. 213-221, 2018.
CR  - D. Mello Souza, J. F. Reichert, V. Ramos do Nascimento and A. Figueiredo Martins, “Ozonation and UV photolysis for removing anticancer drug residues from hospital wastewater,” Journal of Environmental Science and Health, Part A, Vol. 57(8), pp. 635-644, 2022.
CR  - APHA, 2005. Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington, DC.
CR  - H. Ateş and M. E. Argun, “Advanced oxidation of landfill leachate: Removal of micropollutants and identification of by-products,” Journal of Hazardous Materials, Vol. 413, pp. 125326, 2021.
CR  - N. A. Khan, A. H. Khan, S. Ahmed, I. H. Farooqi, S. S. Alam, I. Ali,... and M. Mubashir, “Efficient removal of ibuprofen and ofloxacin pharmaceuticals using biofilm reactors for hospital wastewater treatment,” Chemosphere, Vol. 298, pp. 134243, 2022.
CR  - A. Majumder, B. Gupta, A.K. Gupta, “Pharmaceutically active compounds in aqueous environment: a status, toxicity and insights of remediation,” Environmental Research, Vol. 176, pp. 108542, 2019.
CR  - R. Andreozzi, V. Caprio, R. Marotta, D. Vogna, “Paracetamol oxidation from aqueous solutions by means of ozonation and H2O2/UV system,” Water Research, Vol. 37, pp. 993–1004, 2003.
CR  - V. Trousil, J. Palarcik, Z. Blazkova, J. Korlnkova, O. Machalicky, J. Cakl, “Paracetamol and ibuprofen removal from aqueous solutions by ozonation an photochemical process,” Environmental Protection Engineering, Vol. 44, pp. 159–175, 2018.
CR  - L., Luo, Z., Sun, Y., Chen, H., Zhang, Y., Sun, D., Lu, and J. Ma, “Catalytic ozonation of sulfamethoxazole using low-cost natural silicate ore supported Fe2O3: influencing factors, reaction mechanisms and degradation pathways,” RSC advances, Vol. 13(3), pp. 1906-1913, 2023.
CR  - M. M. Sein, M. Zedda, J. Tuerk, T. C. Schmidt, A. Golloch and C. Von Sonntag, “Oxidation of diclofenac with ozone in aqueous solution,” Environmental science & technology, Vol. 42(17), pp. 6656-6662, 2008.
CR  - J.C.C. da Silva, J.A.R. Teodoro, R.J. Afonso, S.F. Aquino, R. Augusti, “Photolysis and photocatalysis of ibuprofen in aqueous medium: characterization of by-products via liquid chromatography coupled to high-resolution mass spectrometry and assessment of their toxicities against Artemia Salina,” Journal of Mass Spectrometry, Vol. 49, pp. 145–153, 2014.
CR  - W. Dong, S.P. Sun, X. Yang, K. Zhou, Y. Li, X. Wang, Z. Wu, W.D. Wu, X.D. Chen, “Enhanced emerging pharmaceuticals removal in wastewater after biotreatment by a low-pressure UVA/FeIII-EDDS/H2O2 process under neutral pH conditions,” Chemical Engineering Journal, Vol. 366, pp. 539–549, 2019.
CR  - M. Olak-Kucharczyk, M. Foszpanczyk, R. Zyłła, S. Ledakowicz, “Photodegradation and ozonation of ibuprofen derivatives in the water environment: Kinetics approach and assessment of mineralization and biodegradability,” Chemosphere, Vol.  291, pp. 132742, 2022.
CR  - S.K. Alharbi, W.E. Price, J. Kang, T. Fujioka, and L. D. Nghiem, “Ozonation of carbamazepine, diclofenac, sulfamethoxazole and trimethoprim and formation of major oxidation products,” Desalination and Water Treatment, Vol. 57(60), pp. 29340-29351, 2016.
CR  - R.F. Dantas, S. Contreras, C. Sans, and S. Esplugas, “Sulfamethoxazole abatement by means of ozonation,” Journal of hazardous materials, Vol. 150(3), pp. 790-794, 2008.
CR  - L.F. Zhai, H.Y. Guo, Y.Y. Chen, M. Sun, S. Wang, “Structure-dependent degradation of pharmaceuticals and personal care products by electrocatalytic wet air oxidation: a study by computational and experimental approaches,” Chemical Engineering Journal, Vol.423, pp. 30167, 2021.
CR  - S. Aleksić, A. Žgajnar Gotvajn, K. Premzl, M. Kolar, S. Š. Turk, “Ozonation of amoxicillin and ciprofloxacin in model hospital wastewater to increase biotreatability,” Antibiotics, Vol. 10(11), pp. 1407, 2021.
CR  - R., Anjali and S. Shanthakumar, “Synergistic effects on the degradation of a mixture of pharmaceutical pollutants in aqueous solution, raw sewage, and hospital wastewater using UV light-assisted Fenton process,” Journal of Water Process Engineering, Vol. 54, pp. 104025, 2023.
CR  - C. Rodrigues-Silva, R. S. Porto, S. G. D. Santos, J. Schneider and S. Rath, “Fluoroquinolones in hospital wastewater: analytical method, occurrence, treatment with ozone and residual antimicrobial activity evaluation,” Journal of the Brazilian Chemical Society, Vol. 30, pp 1447-1458, 2019.
CR  - J. Ferre-Aracil, Y. Valcárcel, N. Negreira M. L. de Alda, D. Barceló, S. C. Cardona and J. Navarro-Laboulais, “Ozonation of hospital raw wastewaters for cytostatic compounds removal. Kinetic modelling and economic assessment of the process,” Science of The Total Environment, Vol. 556, pp. 70-79, 2016.
CR  - Arslan, S. Veli, and D. Bingöl, “Use of response surface methodology for pretreatment of hospital wastewater by O3/UV and O3/UV/H2O2 processes,” Separation and Purification Technology, Vol. 132, pp. 561-567, 2014.
UR  - https://doi.org/10.35208/ert.1511061
L1  - https://dergipark.org.tr/en/download/article-file/4047943
ER  -