@article{article_1520281, title={Design and Development of Conjugated Polymer Nanoparticles-Based Biosensor for Pesticide Detection}, journal={Necmettin Erbakan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi}, volume={7}, pages={294–308}, year={2025}, author={Yeniterzi, Dilara and Soyler, Dilek and Söylemez, Saniye}, keywords={Conjugated Polymer Nanoparticles, Electrochemical Sensor, Pesticide Detection}, abstract={Because pesticides are linked to various health issues, their residues in edible foodstuffs and water sources are a serious global issue. For this purpose, there is an urgent requirement for the development of sensing systems for the recognition of pesticides from food and vegetables prior to being consumed. By using the nanoparticles of poly(3-hexylthiophene) nanoparticles (P3HTNPs) and poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOTNPs), an electrochemical acetylcholinesterase (AChE) based biosensor was created in response to this challenge. To create such a sensor (PEDOTNPs/P3HTNPs/AChE), P3HTNPs were synthesized and characterized in terms of DLS (Dynamic Light Scattering), zeta potential, FE-SEM (Field Emission-Scanning Electron Microscopy), Ultraviolet Visible (UV-Vis) absorption, and fluorescence emission properties. After the combination of P3HTNPs with PEDOTNPs, the desired biosensor was created with high performance towards AThCl. After all optimization studies, analytical performances, electrochemical and surface characteristics of the biosensor were evaluated. PEDOTNPs/P3HTNPs/AChE biosensor has a limit of detection (LOD) and sensitivity values of 0.009 mM and 37.843 μAmM–1cm–2, respectively. In addition, the biosensor has an excellent anti-interfering ability and long-term stability.}, number={2}, publisher={Necmettin Erbakan University}