TY - JOUR T1 - Cytotoxic activity of root, stem, and flower essential oils from Ferula tenuisecta Korovin AU - Barak, Timur Hakan AU - Omirkhanovna, Bekebaeva Madina AU - Servi, Hüseyin AU - Ertekin, Simge AU - Toleppekovna, Nazarbekova Saltanat PY - 2025 DA - June Y2 - 2024 DO - 10.35378/gujs.1521580 JF - Gazi University Journal of Science PB - Gazi University WT - DergiPark SN - 2147-1762 SP - 549 EP - 558 VL - 38 IS - 2 LA - en AB - This study aims to investigate the phytochemical composition and cytotoxic activities of essential oils derived from Ferula tenuisecta Korovin, given its traditional medicinal uses and potential as a source of bioactive compounds. The hydrodistillation method was used to extract essential oils from the root, stem, and flower of F. tenuisecta. The yields were calculated as 1.7 mL of essential oil in the roots, 0.03 mL in the flowers and 0.01 mL in the stems. The chemical composition was analyzed by GC-MS, identifying 41 compounds in flower oil accounting for 92.8% of the total essential oil, 40 in stem oil accounting for 83.6% of the total essential oil, and 35 in root oil accounting for 98.5% of the total essential oil. In flower essential oil, camphor (14.8%) and α-pinene (11.5%) were the predominant compounds. Oxygenated sesquiterpenes (32.8%), oxygenated monoterpenes (30.4%), and monoterpenes (19.6%) were the dominant chemical groups. In stem essential oil, osthole (13.9%) and 4-oxo-β-ionol (12.4%) were the main compounds. Oxygenated sesquiterpenes (38.9%), coumarin (13.9%), and aromatics (11.0%) were the dominant chemical groups. In root essential oil, fenchone (25.3%), α-pinene (18.5%), and fenchol (12.0%) were the predominant compounds. Cytotoxic activity was determined by MTT assay. This is the first comprehensive study analyzing the chemical composition and cytotoxic properties of essential oils derived from three parts of F. tenuisecta. KW - Ferula tenuisecta KW - GC-MS KW - Essential oil KW - Cytotoxicity KW - Cell line CR - [1] Sharma, A. N., Dewangan, H. K., Upadhyay, P. K. “Comprehensive review on herbal medicine: emphasis on current therapy and role of phytoconstituents for cancer treatment”, Chemistry & Biodiversity, 21(3), e202301468, (2024). DOI: https://doi.org/10.1002/cbdv.202301468 CR - [2] Yücel, Ç., Karatoprak, G. Ş., Açıkara, Ö. B., Akkol, E. K., Barak, T. H., Sobarzo-Sánchez, E., Shirooie, S. “Immunomodulatory and anti-inflammatory therapeutic potential of gingerols and their nanoformulations”, Frontiers in Pharmacology, 13, 902551, (2022). DOI: https://doi.org/10.3389/fphar.2022.902551 CR - [3] Barak, T. H., Celep, İ. K., Şentürk, T. B., Bardakci, H., Celep, E., “In vitro anti-aging potential evaluation of Maclura pomifera (Rafin.) Schneider 80% methanol extract with quantitative HPTLC analysis”, Turkish Journal of Pharmaceutical Sciences, 19(4): 400-407, (2022). DOI: https://doi.org/10.4274/tjps.galenos.2021.65087 CR - [4] Uslu, Y., Akbayrak, H., Ortahisar, B. K., Barak, T. H., “The effect of lavender foot baths on postoperative pain and sleep quality in comparison with foot baths with warm water only—A prospective randomized controlled study”, Explore, 20(3): 385-391, (2024). DOI: https://doi.org/10.1016/j.explore.2023.10.003 CR - [5] Barak, T. H., Dogenci, İ., Bardakcı, H. “Evaluation of the essential oil samples that are sold as “Eucalyptus oil” on the market in Türkiye in terms of European Pharmacopoeia 10.0 criteria” Journal of Research in Pharmacy, 27(4): 1463-1473, (2023). DOI: https://doi.org/10.29228/jrp.433 CR - [6] Servi, H., Demir, U., Servi, E. Y., Gundogdu, B., Barak, T. H., “Antiproliferative and Antibacterial Activities of Four Commercial Essential Oil Samples from Boswellia carteri, B. serrata, and two chemotypes of Canarium luzonicum”, Journal of Essential Oil Bearing Plants, 26(1): 79-94, (2023). DOI: https://doi.org/10.1080/0972060X.2023.2165167 CR - [7] Mohammadhosseini, M., Venditti, A., Sarker, S. D., Nahar, L., Akbarzadeh, A, “The genus Ferula: Ethnobotany, phytochemistry and bioactivities—A review”, Industrial Crops and Products, 129: 350–394, (2019). DOI: https://doi.org/10.1016/j.indcrop.2018.12.012 CR - [8] Sonigra, P., Meena, M, “Metabolic profile, bioactivities, and variations in the chemical constituents of essential oils of the Ferula genus (Apiaceae)”, Frontiers in Pharmacology, 11: 608649, (2021). DOI: https://doi.org/10.3389/fphar.2020.608649 CR - [9] Kurmukov, A., Akhmedkhodzhaeva, K. Estrogennuiye lekarstvennuiye preparatui iz rastenii roda Ferula (Estrogenic medicinal preparations of plants in the genus Ferula). Ibn Sino, Tashkent (in Russian), (1994). CR - [10] Sharma, M., Grewal, K., Jandrotia, R., Batish, D. R., Singh, H. P., Kohli, R. K, “Essential oils as anticancer agents: Potential role in malignancies, drug delivery mechanisms, and immune system enhancement”, Biomedicine & Pharmacotherapy, 146: 112514, (2022). DOI: https://doi.org/10.1016/j.biopha.2021.112514 CR - [11] Ertosun, G. B., Ergen, M., Bardakcı, H., Barak, T. H., Suyen, G, “Cholinergic cognitive enhancer effect of Salvia triloba L. essential oil inhalation in rats”, Marmara Medical Journal, 36(3): 361-370, (2023). DOI: https://doi.org/10.5472/marumj.1368345 CR - [12] Üstüner, H., Barak, T. H., Servi, H., Ertekin, S. K., Sen, A., Dinç, M., Özkan, V. K., “Anticancer, antidiabetic, and antioxidant activities of endemic Stachys buttleri Mill. and Stachys pinardii Boiss. essential oils”, Journal of Essential Oil Bearing Plants, 26(6): 1502-1514, (2023). DOI: https://doi.org/10.1080/0972060X.2023.2293960 CR - [13] Youssef, F. S., Mamatkhanova, M. A., Mamadalieva, N. Z., Zengin, G., Aripova, S. F., Alshammari, E., Ashour, M. L., “Chemical profiling and discrimination of essential oils from six Ferula species using GC analyses coupled with chemometrics and evaluation of their antioxidant and enzyme inhibitory potentia”, Antibiotics, 9(8): 518, (2020). DOI: https://doi.org/10.3390/antibiotics9080518 CR - [14] Azarnivand, H., Alikhah-Asl, M., Jafari, M., Arzani, H., Amin, G., Mousavi, S. S. “Comparison of essential oils from Ferula ovina (Boiss.) aerial parts in fresh and dry stages”, Journal of Essential oil Bearing Plants, 14(2): 250-254, (2011). DOI: https://doi.org/10.1080/0972060X.2011.10643929 CR - [15] Karakaya, S., Göger, G., Bostanlik, F. D., Demirci, B., Duman, H., Kilic, C. S., “Comparison of the essential oils of Ferula orientalis L., Ferulago sandrasica Peşmen and Quézel, and Hippomarathrum microcarpum Petrov and their antimicrobial activity”, Turkish Journal of Pharmaceutical Sciences, 16(1): 69, (2019). DOI: https://doi.org/10.4274/tjps.77200 CR - [16] Barak, T. H., Bölükbaş, E., Bardakci, H., “Evaluation of marketed rosemary essential oils (Rosmarinus officinalis L.) in terms of European pharmacopoeia 10.0 criteria”, Turkish Journal of Pharmaceutical Sciences, 20(4): 253, (2023). DOI: https://doi.org/10.4274/tjps.galenos.2022.78010 CR - [17] Elansary, H. O., Abdelgaleil, S. A., Mahmoud, E. A., Yessoufou, K., Elhindi, K., El-Hendawy, S., “Effective antioxidant, antimicrobial and anticancer activities of essential oils of horticultural aromatic crops in northern Egypt”, BMC complementary and Alternative Medicine, 18: 1-10, (2018). DOI: https://doi.org/10.1186/s12906-018-2262-1 CR - [18] Thu, N. T., Tran-Trung, H., Huong, H. T. D., Manh, D. H., Van Trung, H., Tuan, N. H., Ha, D. T., “Chemical compositions and cytotoxicity effects of essential oils from the leaves and pseudostems of Curcuma zedoaroides Chaveer. & Tanee collected in Vietnam”, Journal of Biologically Active Products from Nature, 14(2): 161-170, (2024). DOI: https://doi.org/10.1080/22311866.2024.2341730 CR - [19] Elghwaji, W., El-Sayed, A. M., El-Deeb, K. S., El-Sayed, A. M., “Chemical composition, antimicrobial and antitumor potentiality of essential oil of Ferula tingitana L. Apiaceae grow in Libya”, Pharmacognosy Magazine, 13(3): 446-451, (2017). DOI: https://doi.org/10.4103/pm.pm_323_15 CR - [20] Ben Salem, S., Znati, M., Jabrane, A., Casanova, J., Ben Jannet, H., “Chemical composition, antimicrobial, anti-acetylcholinesterase and cytotoxic activities of the root essential oil from the Tunisian Ferula lutea (Poir.) Maire (Apiaceae)”, Journal of Essential Oil Bearing Plants, 19(4): 897-906, (2016). DOI: https://doi.org/10.1080/0972060X.2015.1137238 UR - https://doi.org/10.35378/gujs.1521580 L1 - https://dergipark.org.tr/en/download/article-file/4094123 ER -