TY  - JOUR
T1  - The cytotoxic effects of bio-transformed propolis on healthy and colon cancer cell lines
TT  - Biyotransforme propolisin sağlıklı ve kolon kanseri hücre hatları üzerindeki sitotoksik
AU  - Selvi Günel, Nur
AU  - Oktay Çelebi, Latife Merve
AU  - Memmedov, Hikmet
AU  - Durmaz, Burak
AU  - Kalkan Yıldırım, Hatice
AU  - Yıldırım Sözmen, Eser
PY  - 2025
DA  - March
Y2  - 2024
DO  - 10.19161/etd.1524052
JF  - Ege Tıp Dergisi
JO  - EJM
PB  - Ege University
WT  - DergiPark
SN  - 1016-9113
SP  - 77
EP  - 91
VL  - 64
IS  - 1
LA  - en
AB  - Aim: Propolis has a lot of properties related to human health: antioxidant, antimicrobial, antiseptic, antibacterial, anti-inflammatory, antimutagenic, and immunomodulating. Its possible effects on different cancers are among the priorities of these properties. In this study, the comparative cytotoxic effects of bio-transformed propolis samples on colon cancer cells and healthy colon epithelium cells were investigated.Materials and Methods: Lactobacillus plantarum strains used in this study are based on their well-documented ability to metabolize phenolic compounds during fermentation, effectively modifying their structure and activity. These strains exhibit cinnamoyl esterase activity, which is critical for reducing allergenic compounds such as DMEA and CAPE in propolis. Additionally, L. plantarum is widely utilized in biotransformation processes due to its safety, adaptability, and efficiency in enhancing the bioactive properties of natural products, making it an ideal candidate for optimizing the cytotoxic potential of propolis against colon cancer cells.Results: The highest reduction was determined for ferulic acid, and the lowest reduction was obtained by using ethanol as a solvent using ultrasound treatments in the presence of water at optimal conditions (300 W/40 kHz). It was found that propolis samples showed dose-dependent cytotoxic effects on the colon cancer cell line (HCT-116) and healthy colon epithelium cell line (CCD-841 CoN) at 24, 48, and 72 hours.Conclusion: Our results show that the method of propolis extraction, and the type of biotransformation reaction are very important in terms of effect on the cytotoxicity of colon cancer cells. Consequently, in this study, the demonstration of the bio-transformed propolis to have the ability to destroy cancerous cells without causing severe damage to healthy cells reveals that it can have the potential that can be used in cancer treatment.
KW  - colon cancer
KW  - propolis
KW  - biotransformation
KW  - cytotoxicity
KW  - polyphenol
N2  - Amaç: Propolisin insan sağlığıyla ilgili birçok özelliği vardır: antioksidan, antimikrobiyal, antiseptik, antibakteriyel, anti inflamatuar, antimutajenik ve immün modülatör. Farklı kanserler üzerindeki olası etkileri bu özelliklerin öncelikleri arasındadır. Bu nedenle, bu çalışmada biyotransforme edilmiş propolis örneklerinin kolon kanseri hücreleri ve sağlıklı kolon epitel hücreleri üzerindeki karşılaştırmalı sitotoksik etkileri araştırılmıştır.Gereç ve Yöntem: Farklı çözücüler (etanol; polietilen glikol - PEG; su) ve farklı ultrason işlemleri (300 W/40 kHz (5, 10 ve 15 dakika) kullanılarak yapılan ekstraksiyon prosedürüne başlamadan önce, propolis örneklerinin biyotransformasyonu üç farklı L. plantarum suşu (ISLG-2, ATCC-8014 ve Visbyvac) ile çeşitli konsantrasyonlarda (%1,5; %2,5; %3,5) gerçekleştirildi. Örneklerin fenolik profili sıvı kromatografisi-kütle spektrometrisi/kütle spektrometrisi (LCMS/MS) ile analiz edildi. Propolisin HCT-116 ve CCD-841 CoN hücreleri üzerindeki sitotoksik etkileri WST-8 yöntemi ile belirlendi.Bulgular: En yüksek indirgeme ferulik asit için belirlenirken, en düşük indirgeme optimum koşullarda (300 W/40 kHz) su varlığında ultrasonik işlemler kullanılarak çözücü olarak etanol kullanılarak elde edildi. Propolis örneklerinin 24, 48 ve 72. saatlerde kolon kanseri hücre hattı (HCT-116) ve sağlıklı kolon epitel hücre hattı (CCD-841 CoN) üzerinde doza bağlı sitotoksik etkiler gösterdiği bulundu.Sonuç: Propolis ekstraksiyon yönteminin ve biyotransformasyon reaksiyon tipinin kolon kanseri hücrelerinin sitotoksisitesi üzerindeki etki açısından çok önemlidir. Bu çalışmada biyotransforme edilmiş propolisin Sağlıklı hücrelere ciddi zarar vermeden kanserli hücreleri yok edebilen bu yöntemin kanser tedavisinde de kullanılabilecek potansiyele sahip olduğu ortaya çıkarılmıştır.
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UR  - https://doi.org/10.19161/etd.1524052
L1  - https://dergipark.org.tr/en/download/article-file/4105176
ER  -