TY - JOUR T1 - Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia TT - Kolşisin ve Tripsin Uygulamalarının Yetişkin Dişi ve Erkek Nil Tilapya Dokularından ve Larvalarından Elde Edilen Metafaz Kromozom Yayılımı Üzerine Etkileri AU - İnanan, Burak Evren PY - 2024 DA - December Y2 - 2024 DO - 10.58626/menba.1538957 JF - Memba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi PB - Kastamonu University WT - DergiPark SN - 2147-2254 SP - 113 EP - 118 VL - 10 IS - 3 LA - en AB - Assessment of results of different biotechnological methods in aquaculture such as ploidy, gynogenesis, and androgenesis are needed cytogenetical techniques. Achieving well-distributed metaphase chromosome spreads is a preeminent step for cytogenetical techniques. The aim of this study was to evaluate the effects of colchicine and trypsin treatments on the yield of countable metaphase chromosome spreads. With this aim, chromosome preparations from gill, spleen, kidney, and caudal fin tissues of adult females and males Nile tilapia, and also whole body of their larvae were carried out. Based on the previous studies reviewed so far, this is the first systematic and extensive study indicated that the effects of colchicine and trypsin on metaphase chromosome spreads regarding gender differences and larvae. In the chromosome preparations, it was designated four treatments as “Trypsin (-) Colchicine (-)”, “Trypsin (-) Colchicine (+)”, “Trypsin (+) Colchicine (-)”, and “Trypsin (+) Colchicine (+)”. Effects of colchicine and trypsin in terms of their usage in chromosome preparations were evaluated by determining the frequencies of metaphase cells. For this purpose, metaphase index were used to reveal differences in detected metaphase cells among the treatments. The highest metaphase index values were calculated in the Trypsin (+) Colchicine (+) treatment group of all tissues. Also, the scores of metaphase index found in spleen and kidney tissues were greater than those found in gill and fin tissues. The present study has also shown that metaphase chromosome spreads could be obtained without colchicine or trypsin treatments. KW - Metaphase spread KW - Chromosome KW - Colchicine KW - Trypsin KW - Nile Tilapia (Oreochromis niloticus) N2 - Birçok alanda olduğu gibi akuakültürde poliploidi, ginogenez ve androgenez gibi farklı biyoteknolojik yöntemlerin sonuçlarının değerlendirilmesi için sitogenetik tekniklere ihtiyaç duyulmaktadır. Düzenli dağılmış metafaz kromozom yayılımları elde etmek, sitogenetik teknikler için öncelikli bir gerekliliktir. Bu çalışmanın temel amacı kolşisin ve tripsin uygulamalarının, sayılabilir metafaz kromozom yayılımlarının verimi üzerindeki etkilerini değerlendirmektir. Bu amaçla, yetişkin dişi ve erkek Nil tilapyaları solungaç, dalak, böbrek ve kuyruk yüzgeci dokularından ve ayrıca larvalarının tüm vücudundan kromozom preparatları hazırlanmıştır. Önceki çalışmalara göz önüne alındığında, bu çalışma kolşisinin ve tripsinin metafaz kromozom yayılımları üzerindeki etkilerini cinsiyet farklılıkları ve larvalar açısından gösteren ilk sistematik ve kapsamlı çalışmadır. Kromozom preparatlarında “Tripsin (-) Kolşisin (-)”, “Tripsin (-) Kolşisin (+)”, “Tripsin (+) Kolşisin (-)” ve “Tripsin (+) Kolşisin (+)” olmak üzere dört uygulama belirlenmiştir. Kromozom preparatlarının hazırlanmasında, kolşisinin ve tripsinin kullanımları açısından etkileri, metafaz hücrelerinin sayıları belirlenerek değerlendirilmiştir. Bu amaçla, uygulamalar arasında tespit edilen metafaz hücrelerindeki farklılıkları ortaya çıkarmak için metafaz indeksi kullanılmıştır. Tüm dokularda en yüksek metafaz indeksi değerleri Tripsin (+) Kolşisin (+) uygulama grubunda bulunmuştur. 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Bulletin of the Japanese Society of Scientific Fisheries, 47(7): 963. https://doi.org/10.2331/suisan.47.963 UR - https://doi.org/10.58626/menba.1538957 L1 - https://dergipark.org.tr/en/download/article-file/4170709 ER -