TY  - JOUR
T1  - Effects of deltamethrin on photosynthetic pigments and ascorbate-glutathione (ASA-GSH) cycle in Lemna minor
AU  - Aksakal, Özkan
AU  - Uysal, Handan
AU  - Gezgincioğlu, Ebru
PY  - 2024
DA  - December
Y2  - 2024
DO  - 10.51539/biotech.1552098
JF  - Bulletin of Biotechnology
JO  - Bull. Biotechnol.
PB  - Avrasya Araştırma Geliştirme Bilim ve Teknoloji Merkezi Limited Şirketi
WT  - DergiPark
SN  - 2717-8323
SP  - 38
EP  - 43
VL  - 5
IS  - 2
LA  - en
AB  - Deltamethrin is a synthetic pyrethroid insecticide that can cause adverse effects on non-target organisms. This study was designed to investigate the effects of different concentrations (0.001, 0.005 and 0.01 ppm) of deltamethrin on photosynthetic pigments and the ascorbate-glutathione (ASA-GSH) cycle in Lemna minor, a freshwater macrophyte. To assess the effect of deltamethrin on L. minor, photosynthetic pigments, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels, and the activities of some antioxidant enzymes (SOD, CAT and POD) and enzymatic and non-enzymatic antioxidants associated with the ASA-GSH cycle were measured. The results showed that exposure to deltamethrin decreased chl a, chl b and carotenoid levels and increased MDA and H2O2 levels. In addition, deltamethrin exposure significantly increased SOD, CAT and POD activities. The activities of ASA-GSH cycle enzymes (APX, GR, GPX, MDHAR and DHAR) decreased in L. minor exposed to 0.01 ppm deltamethrin, while GST activity increased. Exposure to low doses of deltamethrin increased ASA and GSH levels, while 0.01 ppm deltamethrin decreased the amounts of ASA and GSH compared to the control. Taken together, the present study revealed that different concentrations of deltamethrin inhibited photosynthetic activity, increased lipid peroxidation and caused oxidative stress and activated the antioxidant defense system of L. minor to eliminate the increased oxidative stress.
KW  - Antioxidant enzyme
KW  - Ascorbate
KW  - Deltamethrin
KW  - Glutathione
KW  - Lemna minor
KW  - Malondialdehyde
CR  - Abdelkhalek NKM, Ghazy EW, Abdel-Daim MM. (2015) Pharmacodynamic interaction of Spirulina platensis and deltamethrin in freshwater fish Nile tilapia, Oreochromis niloticus: impact on lipid peroxidation and oxidative stress. Environ Sci Pollut Res 22:3023–3031.
CR  - Agarwal S, Pandey V. (2004) Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biol Plant 48, 555-560.
CR  - Alp FN, Arikan B, Ozfidan-Knakcı C, Gelentürk C, Yildiztugay E, Turan M, Cavusoglu H. (2023) Hormetic activation of nano-sized rare earth element terbium on growth, PSII photochemistry, antioxidant status and phytohormone regulation in Lemna minor. Plant Physiol Biochem 194:361-373.
CR  - Barlow SM, Sullivan FM, Lines J. (2001) Risk assessment of the use of deltamethrin on bednets for the prevention of malaria. Food Chem Toxicol 39:407-422.
CR  - Bashir F, Siddiqi TO, Iqbal M. (2007) The antioxidative response system in Glycine max (L.) Merr. exposed to deltamethrin, a synthetic pyrethroid insecticide. Environ Pollut 147:94–100.
CR  - Duran RE, Kilic S, Coskun Y. (2015) Response of maize (Zea mays L. saccharata Sturt) to different concentration treatments of deltamethrin. Pestic Biochem Phys 124:15-20.
CR  - Foyer CH, Halliwell B. (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21-25.
CR  - Gong Y, Toivonen PMA, Lau OL, Wiersma PA. (2001) Antioxidant system level in ‘Braeburn’ apple in related to its browing disorder. Bot Bul Acad Sin 42:259-264.
CR  - Habig WH, Pabst MJ, Jakoby WB. (1974) Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249(22):7130-7139.
CR  - Hasanuzzaman M, Nahar K, Alam MM, Fujita M. (2012) Exogenous nitric oxide alleviates high temperature induced oxidative stress in wheat (Triticum aestivum L.) seedlings by modulating the antioxidant defense and glyoxalase system. Aust J Crop Sci 6(8):1314-1323.
CR  - Huang C, He W, Guo J, Chang X, Su P, Zhang L. (2005) Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant. J Experiment Bot 56:3041-3049.
CR  - Kumar A, Sasmal D, Bhaskar A, Mukhopadhyay K, Thakur A, Sharma N. (2016) Deltamethrin-induced oxidative stress and mitochondrial caspase-dependent sig- naling pathways in murine splenocytes. Environ Toxicol 31:808–819.
CR  - Li R, Luo C, Qiu J, Li Y, Zhang H, Tan H. (2022) Metabolomic and transcriptomic investigation of the mechanism involved in enantioselective toxicity of imazamox in Lemna minor. J Hazard Mater 425:127818.
CR  - Lu Q, Sun Y, Ares I, Anadón A, Martínez M, Martínez-Larrañaga M-R, Yuan Z, Wang X, Martínez M-A. (2019) Deltamethrin toxicity: A review of oxidative stress and metabolism. Environ Res 170:260-281.
CR  - Miyake C, Asada K. (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33:541-553.
CR  - Nakano Y, Asada K. (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867-880.
CR  - OECD. (2006) OECD guidelines for the testing of chemicals, revised proposal for a new guideline 221, Lemna sp. Growth inhibition test. OECD, Paris.
CR  - Touzout N, Mehallah H, Moralent R, Nemmiche S, Benkhelifa M. (2021) Co‐contamination of deltamethrin and cadmium induce oxidative stress in tomato plants (Solanum lycopersicum L.). Acta Physiol Plant 43:91.
CR  - WHO (World Health Organization). (2010) Specifications and evaluations for public health pesticides- deltamethrin. World Health Organization, Geneva.
CR  - Yee Y, Tam NFY, Wong YS, Lu CY. (2002) Growth and physiological responses of two mangrove species (Bruguira gymnorrhiza and Kandelia candel) to waterlogging. Environ Experiment Bot 1:13.
CR  - Yu C-W, Murphy TM, Lin C-H. (2003) Hydrogen peroxide-induced chilling tolerance in mung beans mediated through ABA-independent glutathione accumulation. Funct Plant Biol 30: 955-963.
UR  - https://doi.org/10.51539/biotech.1552098
L1  - https://dergipark.org.tr/en/download/article-file/4223262
ER  -