TY  - JOUR
T1  - Investigation of Antioxidant/Oxidant Potential of Some Natural Biomaterials
TT  - Bazı Doğal Biyomateryallerin Antioksidan/Oksidan Potansiyelinin Araştırılması
AU  - Güven, Aysel
AU  - Deveci, Haci Ahmet
AU  - Allahverdi, Tülay
AU  - Allahverdi, Ertugrul
AU  - Yavuz Çolak, Meriç
PY  - 2025
DA  - June
Y2  - 2025
DO  - 10.33484/sinopfbd.1554557
JF  - Sinop Üniversitesi Fen Bilimleri Dergisi
JO  - Sinop Uni J Nat Sci
PB  - Sinop University
WT  - DergiPark
SN  - 2536-4383
SP  - 234
EP  - 243
VL  - 10
IS  - 1
LA  - en
AB  - Recent studies have focused on plant-based antioxidative materials and biomaterials that serve as remedies or preventive agents against oxidative stress-induced damage in organisms. Various methods exist to evaluate the antioxidant potential of these compounds. Antioxidants neutralize free radicals in biological cells, mitigating their harmful effects on living organisms. This study aimed to investigate the $in$ $vivo$ protective effects of naringin, silymarin and ellagic acid which are known to contribute to tissue repair following short-term ischemia/reperfusion (I/R) injury. Three-week-old, clinically healthy, Wistar albino rats (n: 35), weighing 250-300 g, were used in this study. Blood samples were analyzed for plasma paraoxonase (PON), total sialic acid (TSA), total antioxidant capacity (TAC) and total oxidant capacity (TOC), oxidative stress index (OSI) and nitric oxide (NO) levels. Results demonstrated that TSA, TOC and NO levels decreased while TAC and PON levels increased following biomaterial administration ($P$
KW  - Free radical
KW  - ischemia/reperfusion
KW  - nitric oxide
KW  - total antioxidant/oxidant capacity
N2  - Son zamanlarda yapılan çalışmaların çoğu, organizmada oksidatif stresin oluşturduğu zararlara çare veya önleyici olarak görev yapan bitki bazlı antioksidatif materyaller ve biyomateryaller üzerine yoğunlaşmıştır. Antioksidanların potansiyel düzeyini değerlendirmek için çeşitli yollar sağlayan farklı yöntemler vardır. Antioksidanlar, biyolojik hücrelerde bulunan ve canlı organizmalar üzerinde olumsuz etkiye sahip olan serbest radikalleri nötralize etmektedir. Bu çalışmanın amacı, kısa süreli iskemi/reperfüzyon (I/R) ile ortaya çıkan hasarlı dokuyu tedavi eden biyomateryaller olarak bilinen naringin, silymarin ve ellagik asitlerin potansiyel koruyucu etkilerinin $in$ $vivo$ olarak incelenmesidir. Bu çalışmada, 3 haftalık, klinik olarak sağlıklı, 250-300 g ağırlığında Wistar albino rat (n: 35) kullanıldı. Sıçanlardan alınan kan örneklerinde Plazma Paraoksonaz (PON), toplam sialik asit (TSA), toplam antioksidan kapasite (TAK) ve toplam oksidan kapasite (TOK), oksidatif stres indeksi (OSI) ve Nitrik Oksit (NO) analiz edildi. Sonuçlar, biyomateryal kullanımından sonra TSA, TOK ve NO düzeylerinin azaldığını, TAK ve PON düzeylerinin ise arttığını ortaya koymaktadır ($P$
CR  - Karimi, M. A., Goudarzi, M., Khodayar, M. J., Khorsandi, L., Mehrzadi, S., & Fatemi, I. (2023). Gemfibrozil palliates adriamycin-induced testicular injury in male rats via modulating oxidative, endocrine and inflammatory changes in rats. Tissue and Cell, 82, 102037. https://doi.org/10.1016/j.tice.2023.102037
CR  - Zimmerman, B.J., Granger, D.N. (1992). Reperfusion injury. Surgical Clinics of North America, 72, 65-83.
CR  - Giorgi, A., Mingozzi, M., Madeo, M., Giovanna, S., Cocucci, M. (2009). Effect of nitrogen starvation on the phenolic metabolism and antioxidant properties of yarrow (Achillea collina Becker ex Rchb). Food Chemistry, 114, 204-211. https://doi.org/10.1016/j.foodchem.2008.09.039
CR  - Innocenti, A., Gülçin, I., Scozzafava, A., Supuran, C. T. (2010). Carbonic anhydrase inhibitors. Antioxidant polyphenols effectively inhibit mammalian isoforms I–XV”, Bioorganic and Medicinal Chemistry Letters 20, 5050-5053, https://doi.org/10.1016/j.bmcl.2010.07.038
CR  - Sharifi-Rad, J., Quispe, C., Castillo, C. M. S., Caroca, R., Lazo-Vélez, M. A., Antonyak, H., Polishchuk, A., Lysiuk, R., Oliinyk, P., De Masi, L., Bontempo, P., Martorell, M., Daştan, S. D., Rigano, D., Wink, M., & Cho, W. C. (2022). Ellagic acid: A review on its natural sources, chemical stability, and therapeutic potential. Oxidative Medicine and Cellular Longevity, https://doi.org/10.1155/2022/3848084
CR  - Shi, R., Gao, D., Stoika, R., Liu, K., Sik, A., & Jin, M. (2022). Potential implications of polyphenolic compounds in neurodegenerative diseases. Critical Reviews in Food Science and Nutrition, 1-24. https://doi.org/10.1080/10408398.2022.2155106
CR  - Eckerson, H. W., Romson, J. O. S. E. P. H., Wyte, C., & La Du, B. N. (1983). The human serum paraoxonase polymorphism: identification of phenotypes by their response to salts. American Journal of Human Genetics, 35(2), 214.
CR  - Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical biochemistry, 37(4), 277-285.
CR  - Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 1103-1111.
CR  - Kösecik, M., Erel, O., Sevinc, E., Selek, S. (2005). Increased oxidative stress in children exposed to passive smoking. International Journal Cardiology, 100, 61-64.
CR  - Sydow, G. (1985). A simplified quick method for determination of sialic acid in serum. Biomedica Biochimica Acta, 44(11-12), 1721-1723.
CR  - Miranda, K. M., Espey, M. G., & Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide: Biology and Chemistry, 5(1), 62–71.
CR  - Jung, G., Hennings, G., Pfeifer, M., & Bessler, W. G. (1983). Interaction of metal-complexing compounds with lymphocytes and lymphoid cell lines. Molecular Pharmacology, 23(3), 698-702.
CR  - Guven, A., Nur, G., & Deveci, H. A. (2019). Effect of green tea (Camellia sinensis L.) and parsley (Petroselinum crispum) diets against carbon tetrachloride hepatotoxicity in albino mice. Fresenius Environmental Bulletin, 28, 6521-6527
CR  - Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., Gargiulo, G., Testa, G., Cacciatore, F., Bonaduce, D., & Abete, P. (2018). Oxidative stress, aging, and diseases. Clinical Interventions in Aging, 13, 757–772.
CR  - Gupta, A., Singh, A. K., Kumar, R., Jamieson, S., Pandey, A. K., & Bishayee, A. (2021). Neuroprotective potential of ellagic acid: a critical review. Advances in Nutrition, 12(4), 1211-1238. https://doi.org/10.1093/advances/nmab007
CR  - Alfei, S., Marengo, B., & Zuccari, G. (2020). Oxidative Stress, Antioxidant Capabilities, and Bioavailability: Ellagic Acid or Urolithins? Antioxidants (Basel, Switzerland), 9(8), 707.
CR  - Li, H. Y., Zhou, D. D., Gan, R. Y., Huang, S. Y., Zhao, C. N., Shang, A., Xu, X. Y., & Li, H. B. (2021). Effects and Mechanisms of Probiotics, Prebiotics, Synbiotics, and Postbiotics on Metabolic Diseases Targeting Gut Microbiota: A Narrative Review. Nutrients, 13(9), 3211. https://doi.org/10.3390/nu13093211
CR  - Deveci, H. A., Nur, G., Ali Kırpık, M., Harmankaya, A., & Yıldız, Y. (2016). Fenolik bileşik içeren bitkisel antioksidanlar. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(1), 26-32.
CR  - Sun, W., & Shahrajabian, M. H. (2023). Therapeutic potential of phenolic compounds in medicinal plants—Natural health products for human health. Molecules, 28(4), 1845. https://doi.org/10.3390/molecules28041845
CR  - Deveci, H. A., Yılmaz, Ş. G., & Deveci, A. (2023). Investigation of the total phenolic compound and total antioxidant/oxidant capacity of methanol extract of Prunus divaricata var (Yonuz plum). Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(2), 1261-1269.
CR  - Deveci, H. A., Doğan, F. H., & Deveci, A. (2025). Investigation of the Total Antioxidant/oxidant Status, Total Phenolic Compounds, and Oxidative Stress Index in Sun-dried Vegetables: Example Gaziantep/Turkiye. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(2), 505-518.
CR  - Amini, N., Maleki, M., & Badavi, M. (2022). Nephroprotective activity of naringin against chemical-induced toxicity and renal ischemia/reperfusion injury: A review. Avicenna Journal of Phytomedicine, 12(4), 357.
CR  - Surai, P. F. (2015). Silymarin as a natural antioxidant: an overview of the current evidence and perspectives. Antioxidants, 4(1), 204-247.
CR  - Singh, H., Singh, T., Buttar, H. S., Kaur, S., Arora, S., Télessy, I. G., & Singh, B. (2022). The Pathophysiology of Liver Disorders and Pharmacotherapy Options with Special Reference to Traditional Herbal Medicines: A Comprehensive Review. Biomedical Translational Research: Drug Design and Discovery, 549-583.
CR  - Guven, A., Nur, G., & Deveci, H. A. (2021). Bıochemıcal and Hıstopathologıcal Profıle of the Lıver in Chemıcal Poısonıng. Guven A (Ed) Editor, Oxidative Stress and Antioxidant Defense System (123-149), Livre De Lyon, Lyon.
CR  - Kanko, M., Maral, H., Akbas, M.H., Ozden, M., Bulbul, S., Omay, O., Yavuz, S., Berki, K.T. (2005). Protective effects of clopidogrel on oxidant damage in a rat model of acute ischemia. The Tohoku Journal of Experimental Medicine, 205:133-139. https://doi.org/10.1620/tjem.205.133
CR  - Avci, G., Kadioglu, H., Sehirli, A. O., Bozkurt, S., Guclu, O., Arslan, E., & Muratli, S. K. (2012). Curcumin protects against ischemia/reperfusion injury in rat skeletal muscle. Journal of Surgical Research, 172(1), e39-e46.
CR  - Singh, D., Chopra, K. (2004). The effect of naringin, a bioflavonoid on ischemia-reperfusion induced renal injury in rats. Pharmacological Research, 25:187-193. https://doi.org/10.1016/j.phrs.2004.01.007
CR  - Jeon, S. M., Bok, S. H., Jang, M. K., Kim, Y. H., Nam, K. T., Jeong, T. S., Park, Y. B., & Choi, M. S. (2002). Comparison of antioxidant effects of naringin and probucol in cholesterol-fed rabbits. Clinica Chimica Acta; International Journal of Clinical Chemistry, 317(1-2), 181–190. https://doi.org/10.1016/s0009-8981(01)00778-1
UR  - https://doi.org/10.33484/sinopfbd.1554557
L1  - https://dergipark.org.tr/en/download/article-file/4233985
ER  -