TY - JOUR T1 - Effect of Different Storage Conditions and Infusion Times on Antioxidant and Antimicrobial Activites and Total Phenolic Content of Rosehip Fruits TT - Farklı Saklama Koşulları ve İnfüzyon Sürelerinin Kuşburnu Meyvelerinin Antioksidan ve Antimikrobiyal Aktiviteleri ile Toplam Fenolik İçeriği Üzerine Etkisi AU - Kılınç, Kağan AU - Darak, Münevver AU - Akar, Zeynep AU - Özad Düzgün, Azer AU - Acet, Tuba AU - Ural, Mustafa Nuri AU - Baltacı, Cemalettin AU - Akmeşe, Osman PY - 2024 DA - December Y2 - 2024 DO - 10.35229/jaes.1567422 JF - Journal of Anatolian Environmental and Animal Sciences JO - JAES PB - Bülent VEREP WT - DergiPark SN - 2548-0006 SP - 711 EP - 720 VL - 9 IS - 4 LA - en AB - Rosa canina L. (Rosehip) is a medicinal plant with strong antioxidant properties.The content of rosehip and accordingly its antioxidant capability may vary according to the way the fruits are used, storage, infusion, freezing and drying methods. In study, it was aimed to determine the antioxidant, antimicrobial activities in rosehip infusion products and extracts under these conditions. Fresh, frozen, dried and +40C stored fruits were infused at 90 degrees and infusion groups were formed. During infusion, samples were taken at 30, 60, 90, 120, 300 and 360th minutes and antioxidant activity (DPPH, FRAP and ABTS methods), total phenolic and total flavonoid contents (TPC and TFC), and antimicrobial activity were analyzed. In addition, phenolic compound content was investigated in 3 different extracts by HPLC method. Dried rosehip (DR) infusing samples were found to have the highest antioxidant activity, TPC and TFC compared to all groups (p-value ranged between 0.000 and 0.05).). Significant decreases were detected in the parameters according to storage and freezing times. A total of 8 phenolic compounds, the highest concentration of which was epicatechin, were detected in aqueous and ethanolic extracts of dried and stored at 40C rosehip (DR+40C) and dried rosehip (DR) fruits. For antimicrobial analysis, Total 7 different Gram (+) and (Gram (-) bacterial strains, were used. While antimicrobial activity was observed in the extracts, no activity was observed in the infusion products. It was determined that antioxidant activity increased during 360 minute infusion time, although it varied according to storage conditions. KW - Antimicrobial KW - Antioxidant KW - Infusion KW - Storage KW - Rosehip N2 - Rosa canina L. (Kuşburnu) güçlü antioksidan özelliklere sahip tıbbi bir bitkidir. Kuşburnunun içeriği ve buna bağlı olarak antioksidan özelliği meyvelerin kullanım şekline, depolama, infüzyon, dondurma ve kurutma yöntemlerine göre değişebilmektedir. Çalışmamızda, sözkonusu koşullar altında kuşburnu infüzyon ürünleri ve ekstraktlarında antioksidan, antimikrobiyal aktivitelerdeki değişimlerin belirlenmesi amaçlandı. Taze, dondurulmuş, kurutulmuş ve +40C'de depolanmış meyveler 90 0C’de demlenmesiyle demleme grupları oluşturuldu. Demleme sırasında 30, 60, 90 ve 120. 300 ve 360. dakikalarda örnekler alınarak ve antioksidan aktivite (DPPH, FRAP ve ABTS yöntemleri ile), toplam fenolik (TPC), toplam flavonoid içerikleri (TFC) ve antimikrobiyal aktiviteleri analiz edildi. Ayrıca 3 farklı ekstraktta HPLC yöntemi ile fenolik bileşik içeriği araştırıldı. Kurutulmuş kuşburnu (DR) demleme örneklerinin tüm gruplara kıyasla en yüksek antioksidan aktiviteye, TPC ve TFC değerlerine sahip olduğu bulundu (p değeri 0.000 ile 0.05 arasında değişmekteydi). Saklama ve dondurma sürelerine göre parametrelerde anlamlı azalmalar tespit edildi. 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