TY  - JOUR
T1  - PHYTOCHEMICAL PROFILE AND SCAVENGING CAPACITY OF BLANCHED CNIDOSCOLUS ACONITIFOLIUS (Mill.) I.M. Johnst AND TELFAIRIA OCCIDENTALIS Hook.fil. LEAVES
AU  - Osundinakin, Michael
AU  - Sharaibi, Olubunmi
AU  - Adekoya, Itunuoluwa
PY  - 2025
DA  - April
Y2  - 2024
DO  - 10.15671/hjbc.1572632
JF  - Hacettepe Journal of Biology and Chemistry
JO  - HJBC
PB  - Hacettepe University
WT  - DergiPark
SN  - 2687-475X
SP  - 71
EP  - 81
VL  - 53
IS  - 2
LA  - en
AB  - This study aimed to investigate the phytochemicals, antioxidant and antimicrobial properties of blanched Cnidoscolus aconitifolius and Telfairia occidentalis. The blanching pretreatment involves immersing the leaves of C. aconitifolius and T. occidentalis in 80 °C water for 3 minutes, followed by a quick cooling process. The concentration of phenol (37.00 ± 1.00) and saponin (21.98 ± 3.75) were significantly (p≤0.05) increased in blanched T. occidentalis, and statistically similar in C. aconitifolius. Blanching reduced the flavonoid content for T. occidentalis (46.32 ± 1.55) and C. aconitifolius (42.42 ± 3.03), while their anthocyanin content remain significantly similar when compared with their respective un-blanched samples. Tannin content was significantly reduced in blanched T. occidentalis (52.04 ± 3.58), and a non-significant reduction was observed for C. aconitifolius (47.70 ± 1.47). The DPPH and FRAP radical scavenging assay at various concentrations (IC25, IC50, IC75, IC100) revealed an increasing distinct trends in C. aconitifolius and T. occidentalis under blanched and un-blanched treatments. For peroxidase, there was no significant difference between the blanched and un-blanched leaves of the two plant species. Blanching significantly increased polyphenol oxidase for C. aconitifolius (73.80 ± 4.51), while T. occidentalis (31.91 ± 1.75) was reduced when compared with their respective counterparts. This study provides valuable information on the effectiveness of the novel blanching techniques in enhancing various attributes of phytochemicals and antioxidant present in C. aconitifolius and T. occidentalis.
KW  - Cnidoscolus aconitifolius
KW  - Telfairia occidentalis
KW  - Blanching
KW  - Enzymes
KW  - Phytochemicals
CR  - J.M. Kong, N.K. Goh, L.S. Chai, T.F. Chia, Recent Advances in Traditional Plant Drugs and Orchids, Acts Pharmacol. Sc., 24 (2008) 7-21.
CR  - D.E. Okwu, N.S. Ukanwa, Nutritive value and phytochemical contents of fluted pumpkin (Telfairia occidentalis Hook f.) vegetable grown with different levels of turkey droppings, African Crop Science Conference Proceedings, 8  (2007) 1759-1964.
CR  - B. Zolfaghari, A. Ghannadi, Research in Medical Sciences, Journal of Isfahan University Medical Science, 6 (2000) 1-6.
CR  - WHO, National Policy on Traditional Medicine and Regulation of Herbal Medicines. – Report of a WHO Global Survey, 2012.
CR  - A. Donkoh, A.G. Kese, C.C. Atuahene, Chemical composition of chaya leaf meal (Cnidoscolus aconitifolius) and availability of its amino acids to chicks, Animal Feed Science and Technology, 30 (1990) 155-162.
CR  - K.K. Pillai, N. Chidambaranathan, M.M. Halith, S. Jayaprakash, N. Narayanan, Anti-hyperglycemic effect of alcoholic extracts of Cnidoscolus chayamansa in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism, International Journal of Research in pharmacy and chemistry, 2(1) (2012) 164-178.
CR  - S. Booth, R. Bressani, T. Johns, Nutrient content of selected indigenous leafy vegetables consumed by the Kekchi people of Alta Verapaz, Guatemala, Journal of Food composition and Analysis, 5(1) (1992) 25-34.
CR  - M.O. Akoroda, Ethnobotany of Telfairia occidentalis (Cucurbitaceae) among Igbos of Nigeria, Econ Bot., 44 (1990) 2939.
CR  - W.B. Akanbi, C.O. Adeboye, A.O. Togun, J.O. Ogunrinde, S.A. Adeyeye, Growth, herbage and seed yield and quality of Telfairia occidentalis as influenced by cassava peel compost and mineral fertilizer, Agric J., 2 (2007) 58895.
CR  - O.A. Oyewole, M.E. Abalaka, Antimicrobial activities of Telfairia occidentalis (fluted pumpkins) leaf extract against selected intestinal pathogens, J Health Sci.,  2 (2012).14.
CR  - O.A. Eseyin, P. Ebong, A. Ekpo, A. Igboasoiyi, E. Oforah, Hypoglycemic effect of the seed extract of Telfairia occidentalis in rat, Pak J Biol Sci., 10 (2007) 498501.
CR  - C. Severini, A. Baiano, T.D. Pilli, B.F. Carbone, A. Derossi, Combined treatments of blanching and dehydration: study on potato cubes, J. Food Eng., 68 (2005) 289–296.
CR  - K. Bahçeci, A. Serpen, V. Gökmen, J. Acar, Study of lipoxygenase and peroxidase as indicator enzymes in green beans: change of enzyme activity, ascorbic acid and chlorophylls during frozen storage, J. Food Eng., 66 (2005) 187–192.
CR  - K.S. Jayaraman, D.K.D. Gupta, Drying of fruits and vegetables, In: A.S. Mujumdar, (Ed.), Handbook of Industrial Drying, third ed, Taylor & Francis, Publishers, 2006, pp. 606–634.
CR  - H.W. Xiao, X.D. Yao, H. Lin, W.X. Yang, J.S. Meng, Z.J. Gao, Effect of SSB (superheated steam blanching) time and drying temperature on hot air impingement drying kinetics and quality attributes of yam slices, J. Food Process Eng., 35 (2012) 370–390.
CR  - X. Shao, Y. Li, Application of low-field NMR to analyze water characteristics and predict unfrozen water in blanched sweet corn, Food Bioprocess Tech., 6 (6) (2013)1593–1599.
CR  - G. Xu, X. Ye, J. Chen, D. Liu, Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract, J Agric Food Chem.,  55 (2007) 330–335.
CR  - G.A. Agbor, J.A. Vinson, P.E. Donnelly, Folin-Ciocalteau Reagent for Polyphenolic Assay, International Journal of Food Science, Nutrition and Dietetics,  23 (2014) 26-33.
CR  - H. Kirk, R. Sawyer, Frait Pearson Chemical Analysis of Food, 8th edition, Longman Scientific and Technical, Edinburgh, 1998, Pp 211-212.
CR  - X. Liu, M. Zho, J. Wang, B. Yang, Y. Jiang, Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China, Journal of Food Composition and Analysis, 21(3) (2008) 219-228.
CR  - R.E. Wrolstad, Color and Pigment Analyses in Fruit Products; Agricultural Experiment Station, Oregon State University, Corvallis, OR, USA, 1993.
CR  - M. Mhada, M. Metougui, K. El Hazzam, K. El Kacimi, A. Yasri, Variations of saponins, minerals and total phenolic compounds due to processing and cooking of quinoa (Chenopodium quinoa Willd.) Seeds, Food, 9(5) (2020) 1–16.
CR  - T.P. Magangana, N.P. Makunga, O.A. Fawole, U.L. Opara, Effect of Solvent Extraction and Blanching Pre-Treatment on Peel Extracts. Process. J., 9 (2021) 10-12.
CR  - E.M. Gonzalez, B. de Ancos, M.O. Cano, Partial Characterization of Polyphenol Oxidase Activity in Raspberry Fruits, J. Agric. Food Chem., 47 (1999) 4068–4072.
CR  - H. Meighani, M. Ghasemnezhad, D. Bakshi, Evaluation of Biochemical Composition and Enzyme Activities in Browned Arils of Pomegranate Fruits, Int. J. Hortic. Sci. Technol., 1 (2014) 53–65.
CR  - A. Karioti, D. Hadjipavlou-Litina, M.L.K. Mensah, T.C. Fleischer, H. Skaltsa, Composition and Antioxidant Activity of the Essential Oils of Xylopia aethiopica (Dun) A. Rich. (Annonaceae) Leaves, Stem Bark, Root Bark, and Fresh and Dried Fruits, Growing in Ghana, J. Agric. Food Chem., 52 (2004) 8094–8098.
CR  - I.F.F. Benzie, J.J. Strain, Determination of optimal extraction conditions for phenolic compounds from: Pistacia atlantica leaves using the response surface methodology, Anal. Methods, 8 (2016) 6107–6114.
CR  - F.A. Ahmed, R.F.M. Ali, Bioactive compounds and antioxidant activity of fresh and processed white cauliflower, Biomed Res Int., (2013).
CR  - Z. Fang, D. Wu, D. Yü, Phenolic compounds in Chinese purple yam and changes during vacuum frying, Food Chem., 128 (2011) 943–948.
CR  - G. Oboh, Effect of blanching on the antioxidant properties of some tropical green leafy vegetables, LWT—Food Sci Technol., 38 (2005) 513–517.
CR  - D. Zhang, Y. Hamauzu, Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking, Food Chem., 88 (2004) 503 509.
CR  - M. Francisco, P. Velasco, D.A. Moreno, Cooking methods of Brassica rapa affect the preservation of glucosinolates, phenolics and vitamin C, Food Res Int., 43 (2010) 1455–1463.
CR  - S.G. Sparg, M.E. Light, J. van Staden, Biological activities and distribution of plant saponins, Journal of Ethnopharmacology, 94 (2-3) (2004) 219–243.
CR  - E. Moghimipour, S. Handali, Saponin: properties, methods of evaluation and applications, Annual Research & Review in Biology, 5 (2014) 207–220
CR  - R. Amarowicz, Tannins: the new natural antioxidants? Eur J Lipid Sci Technol., 109 (2007) 549 551.
CR  - N.P. Uusiku, A. Oelofse, K.G. Duodu, Nutritional value of leafy vegetables  of sub-Saharan Africa and their potential contribution to human health: a review, J Food Compos Anal, 23 (2010) 499–509.
CR  - W. Somsub, R. Kongkachuichai, P. Sungpuag, R. Charoensiri, Effects of three  conventional cooking methods on vitamin C, tannin, myo-inositol phosphates contents in selected Thai vegetables, J Food Compos Anal, 21 (2008) 187–197.
CR  - M. Gaugler, W.J. Grigsby, Thermal degradation of condensed tannins from Radiata pine bark, J Wood Chem Technol., 29 (2009) 305–321.
CR  - V. Kumar, A.K. Sinha, H.P.S. Makkar, K. Becker, Dietary roles of phytate and phytase in human nutrition: a review, Food Chem., 120 (2010) 945–959.
CR  - K. Muraleedharan, S. Kripa, DSC kinetics of the thermal decomposition of copper(II) oxalate by isoconversional and maximum rate (peak) methods, J Therm Anal Calorim., 115 (2014) 1969–1978.
CR  - A. Duval, L. Avérous, Characterization and physicochemical properties of condensed tannins from acacia catechu, J Agric Food Chem., 64 (2016) 1751–1760.
CR  - S.C. Noonan, G.P. Savage, Oxalate content of foods and its effect on humans, Asia Pac J Clin Nutr., 8 (1999) 64–74.
CR  - Y. Porter, Antioxidant properties of green broccoli and purple-sprouting broccoli under different cooking conditions, Biosci Horizons, 5 (2012) 4.
CR  - N. Bernaert, M. De Loose, E. Van Bockstaele, B. Van Droogenbroeck, Antioxidant changes during domestic food processing of the white shaft and green leaves of leek (Allium ampeloprasum var. porrum), J Sci Food Agric., 94 (2014) 1168–1174.
CR  - H.H. Nurhuda, M.Y. Maskat, S. Mamot, J. Afiq, A. Aminah, Effect of blanching on enzyme and antioxidant activities of rambutan (Nephelium lappaceum) peel, Int. Food Res., 20 (2013) 1725–1730.
CR  - M. Rossi, E. Giussani, R. Morelli, R.L. Scalzo, R.C. Nani, D. Torreggiani, Effect of fruit blanching on phenolics and radical scavenging activity of highbush blueberry juice, Food Res Int.,  36 (2003) 999–1005.
CR  - M.R. Marshall, J. Kim, C.I. Wei, Enzymatic browning in fruits, vegetables and seafoods. Food and Agriculture Organization of the United Nations, 2000.
CR  - C. Severini, A. Baiano, T. De Pilli, Prevention of enzymatic browning in sliced potatoes by blanching in boiling saline solutions, LWT- Food Sci Technol 36 (2003) 657–665.
CR  - H.A. Eissa, G.M. Fouad, A.E.A. Shouk, Effect of some thermal and chemical pre-treatments on smoked oyster mushroom quality, Int J Food Sci Technol., 44 (2009) 251–261.
CR  - A.R. Lespinard, S.M. Goñi, P.R. Salgado, Experimental determination and modelling of size variation, heat transfer and quality indexes during mushroom blanching, J Food Eng., 92 (2009) 8-17.
CR  - C. Ndiaye, S.Y. Xu, Z. Wang, Steam blanching effect on polyphenoloxidase, POD and colour of mango (Mangifera indica L.) slices, Food Chem., 113 (2009) 92–95.
CR  - L. Lin, F. Lei, D. Sun, Thermal inactivation kinetics of Rabdosia serra (Maxim.) Hara leaf POD and polyphenol oxidase and comparative evaluation of drying methods on leaf phenolic profile and bioactivities, Food Chem., 134 (2012) 2021–2029.
CR  - K.H.S. Farvin, C. Jacobsen, Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast Food Chemistry, 138 (2013) 1670–1681.
CR  - B. Sultana, F. Anwar, S. Iqbal, Effect of different cooking methods on the antioxidant activity of some vegetables from Pakistan, Int J Food Sci Technol., 43 (2008) 560–567.
CR  - A.M. Jiménez-Monreal, L. García-Diz, M. Martínez-Tomé, Influence of cooking methods on antioxidant activity of vegetables, J Food Sci., 74 (2009) 97–103.
UR  - https://doi.org/10.15671/hjbc.1572632
L1  - https://dergipark.org.tr/en/download/article-file/4311047
ER  -