TY  - JOUR
T1  - Effects of Epigallocatechin-3-Gallate in Preventing 5-Fluorouracil-induced Liver Injury in AML-12 Cell Line
AU  - Akıncı, Melek
AU  - Oltulu, Çağatay
AU  - Bakar, Elvan
AU  - Cevikelli Yakut, Zatiye Ayca
PY  - 2025
DA  - August
Y2  - 2025
DO  - 10.55262/fabadeczacilik.1576263
JF  - Fabad Eczacılık Bilimler Dergisi
JO  - Fabad J Pharm Sci
PB  - FABAD Ankara Eczacılık Bilimleri Derneği
WT  - DergiPark
SN  - 1300-4182
SP  - 259
EP  - 270
VL  - 50
IS  - 2
LA  - en
AB  - This study aimed to assess the potential of epigallocatechin-3-gallate (EGCG) in mitigating 5-fluorouracil (5-FU)-induced hepatotoxicity using the AML12 cell line. In our study, we utilized the AML-12 cell line and divided it into four groups: control, 5-FU, EGCG, and EGCG+FU. The IC50 values were determined using the MTT assay. Expression levels of antioxidant system-associated genes, including Glutathione, catalase, and superoxide dismutase, were analyzed through qRT-PCR. Additionally, qRT-PCR analysis was employed to evaluate the expression of apoptosis-associated genes such as Caspase (Cas)-9, Apaf-1, Cas-3, Bax, and Bcl-2. Furthermore, the gene expressions of p53 and the smac/DIABLO were investigated. When EGCG was administered together with 5-FU GSH, SOD, and CAT mRNA expression levels increased. 5-FU administration statistically significantly elevated smac/DIABLO, Bax, Apaf-1, Bcl-2, and Cas-3 mRNA expression levels by stimulating apoptosis. When EGCG was administered together with 5-FU, Cas-9, Bax, Apaf-1, p53, Cas-3 and smac/DIABLO mRNA expressions elevated significantly, indicating the elimination of damaged structures through apoptosis. In conclusion, our findings indicate that EGCG confers hepatoprotective effects against 5-FU-induced damage through its antioxidant properties. Moreover, EGCG enhances the anticancer efficacy of 5-FU by promoting apoptosis, thereby facilitating the elimination of damaged cells. These results suggest a potential therapeutic synergy between EGCG and 5-FU in managing liver injury and cancer treatment.
KW  - 5-Fluorouracil
KW  - epigallocatechin-3-gallate
KW  - hepatoprotective effect
KW  - oxidative stress
KW  - apoptosis
CR  - Robinson, S. M., Wilson, C. H., Burt, A. D., Manas,
D. M., & White, S. A. (2012). Chemotherapyassociated
liver injury in patients with colorectal
liver metastases: A systematic review and metaanalysis.
Annals of Surgical Oncology, 19, 4287–
4299. https://doi.org/10.1245/s10434-012-2438-8
CR  - Tam, N. N. C., Gao, Y., Leung, Y. K., & Ho, S. M.
(2003). Androgenic regulation of oxidative
stress in the rat prostate involvement of NAD(P)
H oxidases and antioxidant defense machinery
during prostatic involution and regrowth.
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https://doi.org/10.1016/S0002-9440(10)63606-1
UR  - https://doi.org/10.55262/fabadeczacilik.1576263
L1  - https://dergipark.org.tr/en/download/article-file/4326695
ER  -