@article{article_1599737, title={Gene Expressions Associated with Neurotransmitter Metabolism in Children with Attention Deficit Hyperactivity Disorder}, journal={Meandros Medical And Dental Journal}, volume={26}, pages={19–32}, year={2025}, DOI={10.69601/meandrosmdj.1599737}, author={Akalın, Hilal and Erdem, Yakut and Eröz, Recep and Şahin, İzem Olcay and Gökçe, Nuriye and Özmen, Sevgi and Doğan, Muhammet Ensar and Dündar, Munis and Özkul, Yusuf}, keywords={ADHD, methylphenidate, gene expression, qPCR}, abstract={Objective: To investigate the expression levels of genes (SLC6A3, SLC6A4, SLC1A2, SLC18A2, MAOA, COMT, GLYAT, GRM5, DRD4, TPH1, and ADRA2C) associated with attention deficit hyperactivity disorder (ADHD) by pre and post-treatment with methylphenidate to see if they may serve as biomarkers in the etiopathogenesis of diseases. Materials and Methods : Thirty-five ADHD-diagnosed children and 38 healthy controls were included and divided three groups as control, pre-treatment and post-treatment group. After the isolation of mRNA from peripheral blood samples of participants, qPCR was performed using cDNA obtained from mRNA via reverse transcription method. Result: Elevated SLC6A3 and decreased SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, COMT, GLYAT, DRD4 and TPH1 genes’ expression levels of childiren with ADHD were detected (p <0.01). In post-treatment group, while SLC6A3 and COMT expression levels decreased, the expression levels of SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, GLYAT, GRM5 and TPH1 significantly increased (p <0.01). Highlighted relationships among gene expressions levels of control, pre-treatment, and post-treatment groups were detected. Additionally, cut-off values with diagnostic power for SLC6A3, SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, COMT, GLYAT, DRD, and TPH1 were detected. Conclusion: The expression levels of these genes may play an important role in the formation, prognosis and etiopathogenesis of the disease, and these changes can also be used as biomarkers in the differential diagnosis and development of treatment strategies for the disease.}, number={1}, publisher={Aydin Adnan Menderes University}, organization={The study was supported by Erciyes University project TSD-12-4112}