@article{article_160747, title={Molecular and Microscopic Detection of Microsporidia in Some}, journal={Turkish Journal of Agricultural and Natural Sciences}, volume={1}, pages={450–453}, year={2014}, author={Tekın, Olgay and Sezer, Duran and Sogancı, Kubra and Ivgın Tunca, Rahsan}, keywords={Microsporidia, Vairimorpha, Nosema bombycis, PZR, İpekböceği (Bombyx mori L.)}, abstract={ The silkworm, an easy to grow and quite precious insect, is affected by many diseases like other organisms. The microsporidia spores caused to Pebrine which is quite dangerous and causes serious economic damage. In this study, 450 silkworm eggs ( Bombyx mori L.) collected from Bursa province in Turkey were fed into ten different groups until the 4th and 5th larval stages. Nine groups were fed with unwashed mulberry leaves whereas only one group was fed with washed ones. The samples at the 4th and 5th larval stages from each group were screened for N. bombycis and Vairimorpha sp. using both microscopic and molecular methods. There were no nosema spores detected in dyed samples under microscope (100Xobjective). The primers, specific for N. bombycis NBEF35F, NBEF957R and Vairimorpha sp. M1196F, M11822R classified into microsporidia, were used for detection of disease from isolated DNAs. There was no pathogens detected using molecular technique. In conclusion, considering that infection is due to horizontal transmission, the study results may demonstrate that food materials and silkworm from breeding region are safe for disease pathogens. This study is the first molecular application for detection of N. bombycis and Vairimorpha sp . in Turkey. }, number={4}, publisher={Mevlüt AKÇURA}