TY  - JOUR
T1  - Protective effects of lupeol on acute exercise-related  oxidative stress in male rats
AU  - Çoknaz, Hakkı
AU  - Özdemir, Emrah
AU  - Sener, Sıla Özlem
AU  - Özgen, Ufuk
AU  - Gök, Ali
PY  - 2025
DA  - July
Y2  - 2024
DO  - 10.29228/jrp.875
JF  - Journal of Research in Pharmacy
JO  - J. Res. Pharm.
PB  - Marmara University
WT  - DergiPark
SN  - 2630-6344
SP  - 2008
EP  - 2016
VL  - 28
IS  - 6
LA  - en
AB  - The aim of this study is to determine the protective effects of lupeol on acute exhaustive exercise-induced oxidative stress in male rats. Rats were randomly selected and divided into four groups (n=6/group): Control, exercise, lupeol and exercise+lupeol groups. At the end of the exercise protocol, the rats were sacrificed by cervical dislocation, blood samples were taken. The levels of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione (GSH), coenzyme Q10 (CoQ10) and α-lipoic acid (ALA) and oxidative stress levels in serum samples were measured. As a result, it has been determined that lupeol, a natural triterpenoid compound, contributes positively to the level of antioxidants produced by the body and helps suppress oxidants. It has been observed that free radicals caused by oxidative stress that occur as a result of high-intensity exercise are reduced by lupeol. Accordingly, it has been determined that the use of lupeol during exercise in experimental animals exposed to oxidative stress inhibits free radicals formed by stress. In the light of these results, it is thought that lupeol may be included in the composition of pharmaceutical products against exercise-induced oxidative damage.
KW  - Antioxidant
KW  - exercise
KW  - free radical
KW  - lupeol
KW  - oxidative stress
CR  - [1] Akgün N. Egzersiz Fizyolojisi. Third ed., Gökçe Ofset Matbaacılık, Ankara, 1989.
CR  - [2]  Leaf DA, Kleinman MT, Hamilton M, Barstow TJ. The effect of exercise intensity on lipid peroxidation. Med Sci Sports Exerc. 1997; 29(8): 1036-1039. https://doi.org/10.1097/00005768-199708000-00008
CR  - [3] Potteiger JA, Schroeder JM, Goff KL. Influence of music on ratings of perceived exertion during 20 minutes of moderate exercise. Percept https://doi.org/10.2466/pms.2000.91.3.848 Mot Skills. 2000; 91(3 Pt 1): 848-854.
CR  - [4] Urso ML, Clarkson PM. Oxidative stress, exercise and antioxidant supplementation. Toxicology. 2003; 189(1-2): 41 54. https://doi.org/10.1016/s0300-483x(03)00151-3
CR  - [5] Çelik A, Varol R, Onat T, Dağdelen Y, Tugay F. Akut egzersizin futbolcularda antioksidan sistem parametrelerine etkisi. Beden Eğitimi ve Spor Bilimleri Dergisi. 2007; 4: 167-172.
CR  - [6] Önal S. Oksidatif stres ve egzersiz. In: Karaduman A, Ülger Ö, Vardar Yağlı N, Kılınç M, Serel Arslan S. (Eds). Fizyoterapistler ve Öğrenciler İçin e-kitap. Hipokrat Kitabevi, Ankara, 2016; pp. 57-65.
CR  - [7] Karabulut H, Gülay MŞ. Antioksidanlar. https://doi.org/10.24880/maeuvfd.260790 MAE Vet Fak Derg. 2016; 1(1): 65-76.
CR  - [8]  Poljsak B, Šuput D, Milisav I. Achieving the balance between ROS and antioxidants: When to use the synthetic antioxidants. Oxid Med Cell Longev. 2013; 2013: 956792. https://doi.org/10.1155/2013/956792
CR  - [9] Gallo MB, Sarachine MJ. Biological activities of lupeol. Int J Biomed Pharm Sci. 2009; 3(1): 46-66.
CR  - [10] Saleem M. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett. 2009; 285(2): 109 115. https://doi.org/10.1016/j.canlet.2009.04.033
CR  - [11] Sener SÖ, Özgen U, Kanbolat S, Korkmaz N, Badem M, Hanci H, Dirmenci T, Arabaci T, Aliyazıcıoğlu R, Iscan GS. Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity. S Afr J Bot. 2021; 141: 243-254. https://doi.org/10.1016/j.sajb.2021.05.004
CR  - [12] Majumder S, Ghosh A, Bhattacharya M. Natural anti-inflammatory terpenoids in Camellia japonica leaf and probable biosynthesis pathways of the metabolome. Bull Natl Res Cent. 2020; 44(1): 141. https://doi.org/10.1186/s42269 020-00397-7
CR  - [13] Ji LL, Dillon D, Wu E. Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am J Physiol. 1990; 258: 918-923. https://doi.org/10.1152/ajpregu.1990.258.4.R918
CR  - [14] Bejma J, Ramires P, Ji LL. Free radical generation and oxidative stress with ageing and exercise: Differential effects in the myocardium and liver. Acta Phpsiol Scond. 2000; 169: 343-351. https://doi.org/10.1046/j.1365 201x.2000.00745.x
CR  - [15] Park JS, Rehman IU, Choe K, Ahmad R, Lee HJ, Kim MO. A triterpenoid lupeol as an antioxidant and anti neuroinflammatory agent: Impacts on oxidative stress in alzheimer's disease. Nutrients. 2023; 15(13): 3059. https://doi.org/10.3390/nu15133059
CR  - [16] Wenzel DG, Emick GH. An investigation of triterpenes as steroid hormones. J Am Pharm Assoc. 1956; 45(5): 284 287. https://doi.org/10.1002/jps.3030450504
CR  - [17] Zergeroğlu AM, Ersöz G, Yavuzer S. Dayanıklılık antrenmanlarında antioksidan savunma. Hacettepe J Sport Sci. 1997; 8(4): 25-31.
CR  - [18] Burneiko RC, Diniz YS, Galhardi CM, Rodrigues HG, Ebaid GM, Faine LA, Padovani CR, Cicogna AC, Novelli EL. Interaction of hypercaloric diet and physical exercise on lipid profile, oxidative stress and antioxidant defenses. Food Chem Toxicol. 2006 Jul;44(7):1167-1172. https://doi.org/10.1016/j.fct.2006.01.004
CR  - [19] Ji LL. Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc. 1993; 25(2): 225-231.
[20] Elosua R, Molina L, Fito M, Arquer A, Sanchez-Quesada JL, Covas MI, Ordoñez-Llanos J, Marrugat J. Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women. Atherosclerosis. 2003; 167(2): 327-334. https://doi.org/10.1016/s0021 9150(03)00018-2
CR  - [21] Clarkson PM. Antioxidants and physical performance. Crit Rev Food Sci Nutr. 1995; 35: 131-141. https://doi.org/10.1080/10408399509527692
CR  - [22] Leeuwenburgh C, Hollander J, Leichtweis S, Griffiths M, Gore M, Ji LL. Adaptations of glutathione antioxidant system to endurance training are tissue and muscle fiber specific. Am J Physiol. 1997; 272(1 Pt 2): 363-369. https://doi.org/10.1152/ajpregu.1997.272.1.R363
CR  - [23] Kostaropoulos IA, Nikolaidis MG, Jamurtas AZ, Ikonomou GV, Makrygiannis V, Papadopoulos G, Kouretas D. Comparison of the blood redox status between long-distance and short-distance runners. Physiol Res. 2006; 55(6): 611-616. https://doi.org/10.33549/physiolres.930898
CR  - [24] Radák Z, Asano K, Inoue M, Kizaki T, Oh-Ishi S, Suzuki K, Taniguchi N, Ohno H. Superoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. J Appl Physiol (1985). 1995; 79(1): 129-135. https://doi.org/10.1152/jappl.1995.79.1.129
CR  - [25] Sahlin K, Ekberg K, Cizinsky S. Changes in plasma hypoxanthine and free radical markers during exercise in man. Acta Physiol Scand. 1991; 142(2): 275-281. https://doi.org/10.1111/j.1748-1716.1991.tb09157.x
CR  - [26] Vina J, Mina JB, Gomez-Cabrera MC, Lloret A, Marquez R, Pallard FV, Sastre J. Free radicals in exhaustive physical exercise. IUBMB Life. 2000; 50(4-5): 271- 277.
CR  - [27] Ohishi S, Kizaki T, Ookawara T, Toshinai K, Haga S, Karasawa F, Satoh T, Nagata N, Ji LL, Ohno H. The effect of exhaustive exercise on the antioxidant enzyme system in skeletal muscle from calcium-deficient rats. Pflugers Arch. 1998;435(6):767-774. https://doi.org/10.1007/s004240050582
CR  - [28] Koz M, Erbaş D, Bilgihan A, Aricioğlu A. Effects of acute swimming exercise on muscle and erythrocyte malondialdehyde, serum myoglobin, and plasma ascorbic acid concentrations. Can J Physiol Pharmacol. 1992; 70(10): 1392-1395. https://doi.org/10.1139/y92-195
CR  - [29] Gül M, Öztaşan N, Taysi S, Gümüştekin K, Akar S, Bakan N, Dane Ş. Sıçanda oksidatif stres modeli olarak kısa süreli yüzme egzersizi. Hacettepe J Sport Sci. 2001; 12: 26-32.
CR  - [30] Ercan P, El SN. Koenzim Q10’un beslenme ve sağlık açısından önemi ve biyoyararlılığı. TUBAV Bilim Dergisi. 2010; 3(2): 192-200.
CR  - [31] Kwong LK, Kamzalov S, Rebrin I, Bayne AC, Jana CK, Morris P, Forster MJ, Sohal RS. Effects of coenzyme Q(10) administration on its tissue concentrations, mitochondrial oxidant generation, and oxidative stress in the rat. Free Radic Biol Med. 2002;33(5):627-638. https://doi.org/10.1016/s0891-5849(02)00916-4
CR  - [32] Eriksson JG, Forsén TJ, Mortensen SA, Rohde M. The effect of coenzyme Q10 administration on metabolic control in patients with type 2 diabetes mellitus. Biofactors. 1999; 9(2-4): 315-318. https://doi.org/10.1002/biof.5520090229
CR  - [33] Crane FL. Biochemical functions of coenzyme Q10. J Am Coll Nutr. 2001;20(6):591-598. https://doi.org/10.1080/07315724.2001.10719063
CR  - [34] Braun B, Clarkson PM, Freedson PS, Kohl RL. Effects of coenzyme Q10 supplementation on exercise performance, VO2max, and lipid peroxidation in trained cyclists. Int J Sport Nutr. 1991; 1(4): 353-365. https://doi.org/10.1123/ijsn.1.4.353
CR  - [35] Marzatico F, Pansarasa O, Bertorelli L, Somenzini L, Della Valle G. Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes. J Sports Med Phys Fitness. 1997; 37(4): 235-239.
CR  - [36] Powers SK, Criswell D, Lawler J, Ji LL, Martin D, Herb R, Dudley G. Influences of exercise insity and fiber type on antioxidant enzyme actvitiy in rat skeletal muscle. Am J Physiol. 1994; 266: 375-380. https://doi.org/10.1152/ajpregu.1994.266.2.R375
CR  - [37] Turgut G, Demir S, Genç O, Karabulut I, Akalin N. The effect of swimming exercise on lipid peroxidation in the rat brain, liver and heart. Acta Physiol Pharmacol Bulg. 2003; 27(2-3): 43-45.
CR  - [38] Ji LL, Fu R. Responses of glutathione system and antioxidant enzymes to exhaustive exercise and hydroperoxide. J Appl Physiol (1985). 1992; 72(2): 549-554. https://doi.org/10.1152/jappl.1992.72.2.549
CR  - [39] Ji LL, Mitchell EW. Effects of Adriamycin on heart mitochondrial function in rested and exercised rats. Biochem Pharmacol. 1994; 47(5): 877-885. https://doi.org/10.1016/0006-2952(94)90488-x
CR  - [40] Tauler P, Aguiló A, Gimeno I, Guix P, Tur JA, Pons A. Different effects of exercise tests on the antioxidant enzyme activities in lymphocytes and neutrophils. J Nutr Biochem. 2004; 15(8): 479-484. https://doi.org/10.1016/j.jnutbio.2004.03.002
CR  - [41] Knez WL, Jenkins DG, Coombes JS. Oxidative stress in half and full Ironman triathletes. Med Sci Sports Exerc. 2007; 39(2): 283-288. https://doi.org/10.1249/01.mss.0000246999.09718.0c
CR  - [42] Manna I, Jana K, Samanta PK. Intensive swimming exercise-induced oxidative stress and reproductive dysfunction in male wistar rats: protective role of alpha-tocopherol succinate. Can J Appl Physiol. 2004; 29(2): 172-185. https://doi.org/10.1139/h04-013
UR  - https://doi.org/10.29228/jrp.875
L1  - https://dergipark.org.tr/en/download/article-file/4469624
ER  -