TY  - JOUR
T1  - Developing Lucilia sericata Larva on the Natural Liver or Liver Agar
TT  - Doğal Araciğer veya Karaciğer Agarı Üzerinde Gelişen Lucilia sericata Larvası
AU  - Polat, Nevra
AU  - Mollahaliloğlu, Salih
AU  - Koç, Murat
PY  - 2025
DA  - March
Y2  - 2025
DO  - 10.35229/jaes.1608450
JF  - Journal of Anatolian Environmental and Animal Sciences
JO  - JAES
PB  - Bülent VEREP
WT  - DergiPark
SN  - 2548-0006
SP  - 143
EP  - 150
VL  - 10
IS  - 2
LA  - en
AB  - Foods with high protein and organic content are effective in the maturation of sperm and eggs of Calliphoridae and mating. In Lucilia sericata fly colony continuity, liver food in the procedure in terms of both chemical properties and protein and moisture content is a suitable food substrate for females to lay their eggs. However, liver or any other animal tissue can cause contamination due to decomposition during larval development of the eggs. Many sterilised artificial diets have been developed for controlled mass rearing. In this study, natural diet liver and liver-agar artificial diets containing natural diet were preferred after spawning. In particular, the effects of these diets on the following characteristics of L sericata were analysed: 1) growth and development time 2) larval stage length and weight 3) pupal and adult length 4) morphological appearance of larvae, pupae and adults. The experiment was conducted in two replicate experiments with six study groups in each. In general, rearing on liver-based media was similar, but growth and development on sterile liver-agar media was more efficient than on liver and no contamination occurred. There was a significant difference (p0.05).
KW  - Artificial substrate
KW  - growth and development
KW  - larval feeding
KW  - Lucilia sericata
N2  - Calliphoridae üyelerinin sperm ve yumurtalarının olgunlaşmasıyla çiftleşmesinde protein oranı yüksek, organik içerikli besinler etkilidir. Lucilia sericata sinek koloni sürekliliğinde hem kimyasal özelliği hem de protein ve nem içeriği bakımından prosedürdeki ciğer besini dişilerin yumurtalarını bırakmasında uygun besin substratıdır. Ancak yumurtaların larval gelişiminde ciğer veya herhangi bir hayvansal doku çürümeye bağlı olarak kontaminasyona sebep olabilir. Kontrollü kitlesel yetiştirme için birçok sterilize yapay diyet geliştirilmiştir. Bu çalışmada yumurtlamayı takiben doğal diyet ciğer ile doğal diyet içerikli ciğer-agar yapay diyet tercih edildi. Özellikle bu besin ortamlarında Lucilia sericata’nın şu özellikleri üzerindeki etkileri incelendi: 1) büyüme  ve gelişme zamanları 2) larval dönemsel evre boy ve ağırlıkları 3) pupa ve ergin boyları 4) larva, pupa ve ergin morfolojik görünüm. Deney her birinde altışar çalışma grupları ile tekrar teknik bulunan iki deneme ile sürdürüldü. Genel olarak ciğer bazlı besin ortamlarında yetiştirme benzerdi ancak steril ciğer-agar ortamında büyüme ve gelişim ciğerdeki gelişme kıyasla daha etkili idi ve kontaminasyon gelişmedi. Lucilia sericata larvalarından ciğer ve ciğer-agar ile beslenen birinci dönem larva boy, birinci dönem larva ağırlık, ikinci dönem larva ağırlık, üçüncü dönem larva boy, üçüncü dönem larva ağırlık, pupaya geçiş dönemindeki boy ve ergin dönemindeki boy ölçümleri arasında anlamlı fark elde edilmiştir (p0,05).
CR  - Barnes, K.M. & Gennard, D.E. (2013). Rearing bacteria
and maggots concurrently: a protocol using Lucilia
sericata (Diptera: Calliphoridae) as a model
species. Applied Entomology and Zoology, 48, 247-
253.
CR  - Benbow, M.E., Barton, P.S., Ulyshen, M.D., Beasley,
J.C., DeVault, T.L., Strickland, M.S., & Pechal,
J.L. (2019). Necrobiome framework for bridging
decomposition ecology of autotrophically and
heterotrophically derived organic matter.
Ecological Monographs, 89(1), e01331.
CR  - Benbow, M.E. Pechal, J.L. & Mohr, R.M. (2015).
Community and landscape ecology of carrion.
Carrion Ecology, Evolution, and Their
Applications, 151-186.
CR  - Bomphrey, R.J., Walker, S.M., & Taylor, G.K. (2009).
The typical flight performance of blowflies:
measuring the normal performance envelope of
Calliphora vicina using a novel corner-cube arena.
PLoS One, 4(11), e7852.
CR  - Burkepile, D.E., Parker, J.D., Woodson, C.B., Mills, H.J.,
Kubanek, J., Sobecky, P.A. & Hay, M.E. (2006).
Chemically mediated competition between
microbes and animals: microbes as consumers in
food webs. Ecology, 87(11), 2821-2831.
CR  - Byrd, J.H. & Castner, J.L. (2001). Insects of forensic
importance. Forensic Entomology: The Utility of
Arthropods in Legal Investigations, 43-79.
CR  - Clark, K., Evans, L. & Wall, R. (2006). Growth rates of the
blowfly, Lucilia sericata, on different body tissues.
Forensic science international, 156(2-3), 145-149.
CR  - Cotter, S.C., Kruuk, L.E.B. & Wilson, K. (2004). Costs of
resistance: genetic correlations and potential trade‐
offs in an insect immune system. Journal of
Evolutionary Biology, 17(2), 421-429.
CR  - Çoban, E. (2009). Edirne ili Trakya Üniversitesi Güllapoğlu
Yerleşkesi'nde adli entomoloji yönünden önem
taşıyan Diptera faunasının leş üzerinden toplanması
ve taksonomik yönden incelenmesi.
CR  - Early, M. & Goff, M.L. (1986). Arthropod succession
patterns in exposed carrion on the island of O'Ahu,
Hawaiian Islands, USA. Journal of Medical
Entomology, 23, 520-531.
CR  - El-Moaty, Z.A. & Abd Elmoneim, M.K. (2013).
Developmental variation of the blow fly Lucilia
sericata (Meigen, 1826) (Diptera: Calliphoridae)
by different substrate tissue types. Journal of Asia-
Pacific Entomology, 16(3), 297-300.
CR  - Espra, A. S., Tabugo, S. R. M., Torres, M. A. J., Gorospe,
J. G., Manting, M. M. E., & Demayo, C. G.
(2015). Describing dimorphism in wing shapes in
the blowfly Lucilia sericata Meigen (Diptera:
Calliphoridae) using geometric morphometrics.
Advances in Environmental Biology, 9(19 S4), 64-
71.
CR  - Faris, A.M., West, W.R., Tomberlin, J.K. & Tarone,
A.M. (2020). Field validation of a development
data set for Cochliomyia macellaria (Diptera:
Calliphoridae): estimating insect age based on
development stage. Journal of Medical
Entomology, 57(1), 39-49.
CR  - Firoozfar, F., Moosa-Kazemi, H., Baniardalani, M.,
Abolhassani, M., Khoobdel, M. & Rafinejd, J.
(2011). Mass rearing of Lucilia sericata Meigen
(Diptera: Calliphoridae). Asian Pacific Journal of
Tropical Biomedicine, 1(1), 54-56.
CR  - Gomes, L., Gomes, G., Oliveira, H.G., Sanches, M.R. &
Von Zuben, C.J. (2006). Influence of photoperiod
on body weight and depth of burrowing in larvae of
Chrysomya megacephala (Fabricius) (Diptera,
Calliphoridae) and implications for forensic
entomology. Revista Brasileira de Entomologia,
50, 76-79.
CR  - Greenberg, B. & Kunich, J.C. (2002). Entomology and the
law: flies as forensic indicators. Cambridge
University Press.
CR  - Gunn, A. (2019). Essential forensic biology. John Wiley &
Sons.
CR  - Hassan, A. (2008). Influence of food type on larval growth
in Lucilia sericata, London: School of Biosciences,
University of Westminster; 1-26 p.
CR  - Komo, L. & Charabidze, D. (2021). Balance between larval
and pupal development time in carrion blowflies.
Journal of Insect Physiology, 133, 104292.
CR  - Mohr, R.M. (2012). Female blow fly (Diptera:
Calliphoridae) arrival patterns and consequences
for larval development on ephemeral resources.
Texas A&M University.
CR  - Pallant, J.F. & Tennant, A. (2007). An introduction to the
Rasch measurement model: an example using the
Hospital Anxiety and Depression Scale (HADS).
British Journal of Clinical Psychology, 46(1), 1-18.
CR  - Shaaya, E. & Levenbook, L. (1982). The effects of
starvation and 20-hydroxy-ecdysone on feeding
and pupariation of early 3rd-instar Calliphora
vicina larvae. Journal of İnsect Physiology, 28(8),
683-688.
CR  - Sherman, R.A. (1995). What physicians should know about
Africanized honeybees. Western Journal of
Medicine, 163(6), 541.
CR  - Sherman, R.A. & My-Tien Tran, J.M.A. (1995). A simple,
sterile food source for rearing the larvae of Lucilia
sericata (Diptera: Calliphoridae), Medical and
Veterinary Entomology 9, 393-398.
CR  - Tabachnick, B.G & Fidell, L.S. (2013). Using Multivariate
Statistics (6th ed.). Boston: Pearson Publishing.
CR  - Tachibana, S.I. & Numata, H. (2001). An artificial diet for
blow fly larvae, Lucilia sericata (Meigen) (Diptera:
Calliphoridae). Applied Entomology and Zoology,
36(4), 521-523.
CR  - Tarone, A.M. & Foran, D.R. (2006). Components of
developmental plasticity in a Michigan population
of Lucilia sericata (Diptera: Calliphoridae).
Journal of Medical Entomology, 43(5), 1023-1033.
CR  - Wollina, U., Karte, K., Herold, C. & Looks, A. (2000).
Biosurgery in wound healing–the renaissance of
maggot therapy. Journal of the European Academy
of Dermatology and Venereology, 14(4), 285-289.
UR  - https://doi.org/10.35229/jaes.1608450
L1  - https://dergipark.org.tr/en/download/article-file/4472121
ER  -