TY  - JOUR
T1  - Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği
TT  - Efficacy of Exogenous Abscisic Acid on Cholinesterase Enzyme Activity and Phenolic Compound Variability in Hypericum perforatum calli
AU  - Önlü, Şeyma
PY  - 2025
DA  - July
Y2  - 2025
DO  - 10.18016/ksutarimdoga.vi.1614168
JF  - Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
JO  - KSU J. Agric Nat.
PB  - Kahramanmaras Sutcu Imam University
WT  - DergiPark
SN  - 2619-9149
SP  - 1083
EP  - 1094
VL  - 28
IS  - 4
LA  - tr
AB  - Bu çalışmanın amacı, absisik asitin (ABA) Hypericum perforatum'un in vitro kalluslarının kolinesteraz enzim aktiviteleri ve biyoaktif bileşenleri üzerine etkisini araştırmaktır. Kalluslar, 0.5 mg L-1 thidiazuron (TDZ) ve 0.5 mg L-1 indol bütirik asit (IBA) içeren Linsmaier Skoog (LS)/B5 besiyerinden elde edildi. Doku kültüründe elde edilen kalluslara farklı absisik asit (0,01 mg L-1, 0,05 mg L-1 ve 0,1 mg L-1) uygulamaları yapıldı ve %80'lik metanolde ekstrakte edildi. Hispidulin, fumarik asit, akasetin, epikateşin, naringenin bileşikleri Sıvı Kromatografisi-Yüksek Çözünürlüklü Kütle Spektrometrisi (LC-HRMS) ile analiz edildi. Kallus örneklerinde en yüksek epikateşin (1.09 mg L-1)  0.05 mg L-1 ABA'da bulunurken, fumarik asit (2.30 mg L-1) ve hispidulin (0.78 mg L-1) bileşikleri en yüksek 0.1 mg L-1  ABA içerenlerde tespit edildi. Sadece 0.01 mg L-1  ABA içeren ortamda akasetin (0.10 mg L-1) üretilirken, 0.05 mg L-1  ABA içeren besin ortamında naringenin (0.01 mg L-1) üretildi. En güçlü Asetilkolinesteraz (AchE) (0.207±0.012) ve Bütirilkolinesteraz BChE (0.243±0.019) inhibisyon aktivitesinin 0.1 mg L -1 ABA uygulamasıyla olduğu ortaya çıktı. Bu çalışma, ABA'nın H.perforatum'un kallus kültüründe uyarıcı olarak kullanılabileceğini ve ilgili metabolitlerin miktarlarının elisitör aracılığıyla değiştirilebileceğini göstermiştir.
KW  - Absisik asit
KW  - Kallus üretimi
KW  - Hispidulin
KW  - LC-HRMS
KW  - Kolinesteraz inhibisyonu
N2  - The aim of this study was to investigate the effect of abscisic acid (ABA) on cholinesterase enzyme activities and bioactive components of in vitro calli of Hypericum perforatum. Calli were obtained from Linsmaier Skoog (LS)/B5 medium containing 0.5 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 indole butyric acid (IBA). Different abscisic acid (0.01 mg L-1, 0.05 mg L-1, and 0.1 mg L-1) applications were performed on the calli obtained in tissue culture and extracted in 80% methanol. Hispidulin, fumaric acid, acacetin, epicatechin, and naringenin compounds were analyzed in Liquid chromatography–high–resolution mass spectrometry (LC-HRMS). While epicatechin (1.09 mg L-1) was found at the highest 0.05 mg L-1 ABA in callus samples, fumaric acid (2.30 mg L-1) and hispidulin (0.78 mg L-1) compounds were detected in the highest containing of 0.1 mg L-1 ABA. Acacetin (0.10 mg L-1) was produced in only 0.01 mg L-1 ABA, whereas naringenin (0.01 mg L-1) was produced in medium containing 0.05 mg L-1 ABA. The strongest Acetylcholinesterase (AChE) (0.207±0.012) and Butyrylcholinesterase (BChE) (0.243±0.019) inhibition activity was shown the best with 0.1 mg L-1 ABA application. This study has shown that ABA can be used as an elicitor in callus culture of H. perforatum, and the amounts of related metabolites can be changed via elicitors
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UR  - https://doi.org/10.18016/ksutarimdoga.vi.1614168
L1  - https://dergipark.org.tr/en/download/article-file/4496348
ER  -