TY  - JOUR
T1  - Yeni Tanımlanan Bir Tür Olan Aubrieta alshehbazii Dönmez, Uğurlu ve M.A. Koch’ un Antikarsinojenik Etkilerinin, Antioksidan Özelliklerinin ve Fitokimyasal Bileşenlerinin Tespiti
TT  - Determination of the Anticarcinogenic Effects, Antioxidative Features and Phytochemical Components of a Newly Identified Species Aubrieta alshehbazii Dönmez, Uğurlu & M.A. Koch
AU  - Aksoy, Laçine
AU  - Hazman, Ömer
AU  - Yılmaz, Mustafa Abdullah
AU  - Büyükben, Ahmet
AU  - Kargıoğlu, Mustafa
AU  - Çakal, Beyza
AU  - Gümüş, Beyza
AU  - Efiloğlu, Feyza
PY  - 2025
DA  - June
Y2  - 2025
DO  - 10.18016/ksutarimdoga.vi.1616833
JF  - Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
JO  - KSU J. Agric Nat.
PB  - Kahramanmaras Sutcu Imam University
WT  - DergiPark
SN  - 2619-9149
SP  - 650
EP  - 660
VL  - 28
IS  - 3
LA  - tr
AB  - Aubrieta alshehbazii, Elşahbaz obrizyası olarak bilinen endemik bir türdür. Tanımlanması yeni yapıldığından hakkında pek çalışma bulunmayan bu türün, antioksidatif, antikanserojenik etkileri ile fenolik madde içeriğinin belirlenmesi amacıyla çalışma gerçekleştirilmiştir. Antioksidatif özelliğinin belirlenmesi için DPPH radikal savıcı etkisi, total antioksidan ve oksidan kapasitesi ölçülmüştür. Antikanserojenik etki kapsamında sağlıklı HGF hücreleri ve akciğer kanser (A549) hücreleri kullanılmıştır. Bitkinin içerdiği fenolik maddeler ise LC-ESI-MS/MS ile belirlenmiştir. Analizler sonucunda bitkinin 0.1 µM ve 0.5 µM konsantrasyonda sentetik antioksidan olan BHT den istatistiksel anlamlılıkta (sırasıyla p= 0.008 ve p=0.016)) yüksek bir DPPH radikali savıcı etkiye sahip bulunmuştur. Türün total antioksidan kapasitesi standart antioksidanlardan düşüktür. Düşük konsantrasyonlarda ekstraktın A549 hücreleri üzeirne etkileri görülmezken, yüksek konsantrasyonlarda hem HGF hücreleri ve hem de A549 hücreleri üzerine sitotoksik etki gösterdiği belirlenmiştir. Fenolik içerik analizleri sonucunda yapısında en çok bulunan bileşenler, fumarik asit, kafeik asit, kuersetin, kinik asit, rosmarinik asit ve klorojenik asittir. Sonuç olarak tür radikal savıcı etkisi ile ön plana çıkmaktadır. Bu etkinin hangi bileşenden kaynaklandığının belirlenmesine yönelik çalışmalar yapılması önerilmektedir.
KW  - Aubrieta alshehbazii
KW  - DPPH
KW  - Sitotoksik
KW  - Total antioksidan kapasite
KW  - LC-ESI-MS/MS
N2  - Aubrieta alshehbazii is an endemic species known as Elşahbaz Obrizyası. A study was conducted to determine the antioxidative, anticarcinogenic effects and phenolic substance content of this species, which is not well-studied due to its new definition. DPPH radical scavenging effect, total antioxidant and oxidant capacity were measured to determine its antioxidant properties. Healthy HGF cells and lung cancer (A549) cells were used within the scope of the anticarcinogenic effect. Phenolic substances contained in the plant were determined by LC-ESI-MS/MS. As a result of the analysis, it was found that the plant had a statistically significant (p=0.008 and p=0.016) higher DPPH radical scavenging effect than the synthetic antioxidant BHT at 0.1 µM and 0.5 µM concentrations. The total antioxidant capacity of the species is lower than standard antioxidants. While no effects of the extract on A549 cells were observed at low concentrations, it was determined that it had a cytotoxic effect on both HGF cells and A549 cells at high concentrations. As a result of the phenolic content analysis, the most abundant components in its structure are fumaric acid, caffeic acid, quercetin, quinic acid, rosmarinic acid and chlorogenic acid. As a result, the species stands out with its radical scavenging effect. It is recommended that studies be conducted to determine which component is responsible for this effect.
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UR  - https://doi.org/10.18016/ksutarimdoga.vi.1616833
L1  - https://dergipark.org.tr/en/download/article-file/4507862
ER  -