TY - JOUR T1 - Environmental Threats of Cigarette Waste: Phytotoxic Effects of Leachate from Cigarette Butts on Vigna radiata (L.) R. Wilczek Seeds AU - Aşkın Çelik, Tülay AU - Sadıç, Emine PY - 2025 DA - September Y2 - 2025 DO - 10.31015/2025.3.3 JF - International Journal of Agriculture Environment and Food Sciences JO - int. j. agric. environ. food sci. PB - Gültekin ÖZDEMİR WT - DergiPark SN - 2618-5946 SP - 654 EP - 662 VL - 9 IS - 3 LA - en AB - Cigarette consumption and the associated environmental impact of cigarette waste are on the rise. Among these, cigarette butts particularly due to their cellulose acetate content can persist in the environment for prolonged periods and may exert toxic effects on soil dwelling organisms and plants. This study aimed to evaluate the phytotoxic effects of leachates derived from both regular and light cigarette butts and filters on Vigna radiata (L.) R. Wilczek (mung bean). Experimental groups included a negative control (distilled water), a positive control (0.02 M ethyl methanesulfonate), and treatments with leachates of light cigarette butts, light filters, regular cigarette butts, and regular filters. Leachates were applied at varying concentrations (6.25%, 12.5%, 25%, 50%, and 100%), and their effects were assessed based on seed germination, root and shoot lengths, growth index (%GI), relative growth index (RGI), and fresh and dry biomass. The results revealed significant inhibition of root and shoot growth at concentrations of 25%, 50%, and 100% across all treatment groups. Higher concentrations notably suppressed RGI and %GI values (p < 0.05), while fresh and dry weights declined proportionally with increasing concentration. These findings clearly demonstrate that leachates from both regular and light cigarette butts and filters have phytotoxic effects on plant development. Given the growing concern over environmental pollution, the need for effective strategies to mitigate the ecological risks posed by cigarette waste is evident, underscoring the importance of further research in this area. KW - Biomass KW - Cigarette butt KW - Germination KW - Growth Index KW - Phytotoxicity KW - Vigna radiata CR - Barnabas, B., Jäger, K., & Fehér, A. (2008). The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell & Environment, 31(1), 11–38. Doi: https://doi.org/10.1111/j.1365-3040.2007.01727.x CR - Belzagui, F., Buscio, V., Gutiérrez-Bouzán, C., & Vilaseca, M. (2021). Cigarette butts as a microfiber source with a microplastic level of concern. Science of The Total Environment, 762, 144165. Doi: https://doi.org/10.1016/j.scitotenv.2020.144165 CR - Bhalla, P.R., Whitaker, T.W., & Sabharwal, P.S. (1973). Effect of water soluble tobacco smoke extracts from filter and non-filter cigarettes on seed germination of onion and tomato. Environmental Pollution, 5, 231-236. Doi: https://doi.org/10.1016/0013-9327(73)90016-2 CR - Bishnoi, N.R., Chugh, K., & Sawhney, S.K. (1993). Effect of chromium on photosynthesis, respiration and nitrogen fixation in pea (Pisum sativum L.) seedlings. Journal of Plant Physiology, 142, 25-30. CR - Bishop, K.C., Ketcham, J.D., & Kuminoff, N.V. (2022). Hazed and confused: The effect of air pollution on dementia. NBER Working Paper Series, Working Paper No. 24970. Retrieved from http://www.nber.org/papers/w24970 CR - Bonciu, E., Firbas, P., Fontanetti, C.S., Wusheng, J., Karaismailoğlu, M., … Papini, A. (2018). An evaluation for the standardization of the Allium cepa test as cytotoxicity and genotoxicity assay. Caryologia, 71(3), 191-209. Doi: https://doi.org/10.1080/00087114.2018.1503496 CR - Charles, J., Sancey, B., Crini, M.N., Badot, P., Degiorgi, F., Trunfio, G., & Crini, G. (2011). Evaluation of the phytotoxicity of polycontaminated industrial effluents using the lettuce plant (Lactuca sativa) as a bioindicator. Ecotoxicology and Environmental Safety. Doi: https://doi.org/10.1016/j.ecoenv.2011.07.025 CR - Chatzistathis, T., & Therios, I. (2013). How soil nutrient availability influences plant biomass and how biomass stimulation alleviates heavy metal toxicity in soils: The cases of nutrient use efficient genotypes and phytoremediators, respectively. In E. Anderson (Ed.), Soil Fertility (pp. 18). InTech. Doi: https://doi.org/10.5772/53594 CR - Darabi, K., Hassani, G., Alinejad, N., & Badeenezhad, A. (2023). Spatial and temporal variation of CBPI and leakage of heavy metals from cigarette butts into the urban environment. Scientific Reports, 13, 1424. Doi: https://doi.org/10.1038/s41598-023-28340-6 CR - Dobaradaran, S., Schmidt, T.C., Nabipour, I., Khajeahmadi, N., Tajbakhsh, S., Saeedi, R., Mohammadi, M.J., Keshtkar, M., Khorsand, M., & Faraji Ghasemi, F. (2018). Characterization of plastic debris and association of metals with microplastics in coastline sediment along the Persian Gulf. Waste Management, 78, 649-658. Doi: https://doi.org/10.1016/j.wasman.2018.06.037 CR - Dobaradaran, S., Telgheder, U., De La Torre, G.E., Rockel, S.P., Mutke, X.A.M., & Schmidt, T.C. (2024). Elucidating nicotine transfer into water environments via cigarette butt remaining parts. Environmental Pollution, 341, 122943. Doi: https://doi.org/10.1016/j.envpol.2023.122943 CR - Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S., Ihsan, M.Z., Alharby, H., Wu, C., Wang, D., & Huang, J. (2017). Crop production under drought and heat stress: plant responses and management options. Frontiers in Plant Science, 8, 1147. Doi: https://doi.org/10.3389/fpls.2017.01147 CR - Ganesan, K., & Xu, B. (2018). A critical review on phytochemical profile and health promoting effects of mung bean (Vigna radiata). Food Science and Human Wellness, 7, 11-33. Doi: https://doi.org/10.1016/j.fshw.2018.02.001 CR - Green, D.S., Boots, B., Da Silva, C.J., & Starkey, T. (2019). Cigarette butts have adverse effects on initial growth of perennial ryegrass (Gramineae: Lolium perenne L.) and white clover (Leguminosae: Trifolium repens L.). Ecotoxicology and Environmental Safety, 182, 109418. Doi: https://doi.org/10.1016/j.ecoenv.2019.109418 CR - Guevara, M.D.F., Mello, A.G., Corrêa, E.K., Guedes, H.A.S., Corrêa, L.B., & Nazari, M.T. (2019). Fitotoxicidade em águas residuárias domésticas utilizando sementes como bioindicadores. Revista DAE, 67, 44-51. Doi: https://doi.org/10.4322/dae.2019.014 CR - Guidoni, L.L.C., Marques, R.V., Moncks, R.B., Botelho, F.T., Da Paz, M.F., Corrêa, L.B., & Corrêa, E.K. (2018). Home composting using different ratios of bulking agent to food waste. Journal of Environmental Management, 207, 141-150. Doi: https://doi.org/10.1016/j.jenvman.2017.11.031 CR - Guden, B. (2025). Seedling growth effect of sesame (Sesamum indicum L.) to PEG-induced drought stress and different boron levels. International Journal of Agriculture, Environment and Food Sciences, 9(1), 233-238. Doi: https://doi.org/10.31015/2025.1.25 CR - Güngören, Z., & Başaran, A. (2021). Urla (İzmir/Türkiye) kumsallarında deniz çöplerinin mevsimsel olarak araştırılması. Ege Journal of Fisheries and Aquatic Sciences, 38(1), 53-61. Doi: https://doi.org/10.12714/egejfas.38.1.06. (in Turkish). CR - Haq, I., Kumari, V., Kumar, S., Raj, A., Lohani, M., & Bhargava, R.N. (2016). Evaluation of the phytotoxic and genotoxic potential of pulp and paper mill effluent using Vigna radiata and Allium cepa. Advances in Biology, 2016, 8065736. Doi: https://doi.org/10.1155/2016/8065736 CR - Karaman, R., & Türkay, C. (2022). Türkiye’deki maş fasulyesi [Vigna radiata (L.) Wilczek] genotiplerinin çıkış ve fide özellikleri yönünden karakterizasyonu. Mustafa Kemal University Journal of Agricultural Sciences, 27(3), 434-444. Doi: https://doi.org/10.37908/mkutbd.1086965 (in Turkish). CR - Lee, W., & Lee, C.C. (2015). Developmental toxicity of cigarette butts—an underdeveloped issue. Ecotoxicology and Environmental Safety, 113, 362–368. Doi: https://doi.org/10.1016/j.ecoenv.2014.12.022 CR - Lima, C., Amaral Dos Santos Pinto, M., Brasil Choueri, R., Buruaem Moreira, L., & Braga Castro, I. (2021). Occurrence, characterization, partition, and toxicity of cigarette butts in a highly urbanized coastal area. Waste Management, 131, 10-19. Doi: https://doi.org/10.1016/j.wasman.2021.05.029 CR - Lucia, G., Giuliani, M.E., d'Errico, G., Booms, E., Benedetti, M., Di Carlo, M., & Regoli, F. (2023). Toxicological effects of cigarette butts for marine organisms. Environment International, 173, 107733. Doi: https://doi.org/10.1016/j.envint.2023.107733 CR - Mansouri, N., Etebari, M., Ebrahimi, A., Ebrahim, K., & Hassanzadeh, A. (2020). Genotoxicity and phytotoxicity comparison of cigarette butt with cigarette ash. Environmental Science and Pollution Research, 27, 40383-40391. Doi: https://doi.org/10.1007/s11356-020-10396-6 CR - Mendes, P.M., Becker, R., Corrêa, L.B., Bianchi, I., Dai Prá, M.A., Lucia Jr., T., & Corrêa, E.K. (2016). Phytotoxicity as an indicator of stability of broiler production residues. Journal of Environmental Management, 167, 156-159. Doi: https://doi.org/10.1016/j.jenvman.2015.11.031 CR - Mendes, P.M., Neto, J.C.P., Gonçalvez, L.B., Macedo, S.C., Araujo, T.R., Rodrigues, A.L., Correa, L.B., & Correa, E.K. (2020). Improvement of the phytotoxicological test with lettuce seed germination: reliability and accuracy of the method. Brazilian Journal of Development, 6, 18178–18184. Doi: https://doi.org/10.34117/bjdv6n4.18178 CR - Mendes, P.M., Ribeiro, J.A., Martins, G.A., Lucia Jr., T., Araujo, T.R., Fuentes-Guevara, M.D., Correa, L.B., & Correa, E.K. (2021). Phytotoxicity test in check: Proposition of methodology for comparison of different method adaptations usually used worldwide. Journal of Environmental Management, 291, 112698. Doi: https://doi.org/10.1016/j.jenvman.2021.112698 CR - Mendes, P.M., Toledo, T.S., Forgiarini, J., Cruz, D.B., Guevara, M.D.F., Guidotti, R.M.M., Roll, V.F.B., Araujo, T.R., Correa, L.B., & Correa, E.K. (2019). Evaluation of a new avian bed conditioner used in the period between the creations of different batches of chicken. International Journal of Development Research, 9, 30203–30206. CR - Messrouk, H., Hadj, M.M., Touil, Y., & Amrane, A. (2014). Physico-chemical characterization of industrial effluents from the town of Ouargla (South East Algeria). Energy Procedia, 50, 255–262. Doi: https://doi.org/10.1016/j.egypro.2014.06.034 CR - Muhie, S.H., Memis, N., Ozdamar, C., Gokdas, Z., & Demir, I. (2021). Biostimulant priming for germination and seedling quality of carrot seeds under drought, salt and high temperature stress conditions. Journal of Agriculture, Environment and Food Sciences, 5(3), 352-359. Doi: https://doi.org/10.31015/jaefs.2021.3.13 CR - Moriwaki, H., Kitajima, S., & Katahira, K. (2009). Waste on the roadside, ‘poi-sute’ waste: Its distribution and elution potential of pollutants into environment. Waste Management, 29, 1192–1197. Doi: https://doi.org/10.1016/j.wasman.2008.11.014 CR - Nasab, A.Y., Oskoei, V., Rezanasab, M., Alinejad, N., Hosseinzadeh, A., & Kashi, G. (2022). Cigarette butt littering consequences: a study of pollution rate on beaches and urban environments. Environmental Science and Pollution Research, 29, 45396–45403. Doi: https://doi.org/10.1007/s11356-022-19155-5 CR - National Cancer Institute (2001). Risks associated with smoking cigarettes with low machine measured yields of tar and nicotine. Smoking and Tobacco Control Monograph, 13. CR - Ocaktan, M.E., Çalışkan, D., Kanyılmaz, O., & Yıldız, A. (2008). Ankara Üniversitesi Tıp Fakültesi öğrenci ve çalışanlarında “light” sigara içme sıklığı. Tüberküloz ve Toraks Dergisi, 56, 304–309. (in Turkish). CR - Özkaya, M.S. (2021). Artvin-Genya Dağı Yöresi Doğu Ladini (Picea orientalis Lipsky) ormanlarında topraküstü biyokütlenin belirlenmesi için denklemler geliştirilmesi. Journal of Anatolian Environment and Animal Sciences, 6, 99–105. (in Turkish). CR - Pramanik, S.K. (2021). Impact assessment of tobacco industry wastewater on germination and early seedling growth of mung bean [Vigna radiata (L.) R. Wilczek]. The Agriculturists, 19, 86–94. Doi: https://doi.org/10.3329/agric.v19i1.56741 CR - Shiffman, S., Pillitteri, J.L., & Burton, S.L. (2001). Smokers’ beliefs about “Light” and “Ultralight” cigarettes. Tobacco Control, 10, 17–23. Doi: https://doi.org/10.1136/tc.10.1.17 CR - Slaughter, E., Gersberg, R.M., Watanabe, K., Rudolph, J., Stransky, C., & Novotny, T.E. (2011). Toxicity of cigarette butts, and their chemical components, to marine and freshwater fish. Tobacco Control, 20, 25–29. Doi: https://doi.org/10.1136/tc.2010.036491 CR - Tiquia, S.M., & Tam, N.F.Y. (1998). Elimination of phytotoxicity during co-composting of spent pig manure sawdust litter and pig sludge. Bioresource Technology, 65, 43–49. Doi: https://doi.org/10.1016/S0960-8524(98)00024-8 CR - World Health Organization (2023). World Health Organization report on the global tobacco epidemic, 2023. Monitoring tobacco use and prevention policies. World Health Organization. CR - Yang, S., Gu, C., Jiao, Y., & Yang, Q. (2023). Research on the presence of cigarette butts and their leaching of chemical pollutants and microparticles: The case of Dalian, China. Frontiers in Marine Science, 10, 1277402. Doi: https://doi.org/10.3389/fmars.2023.1277402 CR - Young, B.J., Riera, N.I., Beily, M.E., Bres, P.A., & Crespo, D.C. (2012). Toxicity of the effluent from an anaerobic bioreactor treating cereal residues on Lactuca sativa. Ecotoxicology and Environmental Safety, 76, 182–186. Doi: https://doi.org/10.1016/j.ecoenv.2011.10.002 CR - Zucconi, F., Pera, A., Forte, M., & Bertoldi, M. (1981). Evaluating toxicity in immature compost. Biocycle, 22, 54–57. UR - https://doi.org/10.31015/2025.3.3 L1 - https://dergipark.org.tr/en/download/article-file/4597060 ER -