TY  - JOUR
T1  - Bioaccumulation of Metals and Metallothionein Levels in  Some Fish Species Living in Murat River
TT  - Murat Nehri'nde Yaşayan Bazı Balık Türlerinde Metallerin Biyoakümülasyonu ve Metallothionein Seviyeleri
AU  - Özok, Necati
AU  - Yıldırım, Hülya
PY  - 2025
DA  - August
Y2  - 2025
DO  - 10.53433/yyufbed.1641740
JF  - Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi
JO  - YYU JINAS
PB  - Van Yuzuncu Yıl University
WT  - DergiPark
SN  - 1300-5413
SP  - 445
EP  - 458
VL  - 30
IS  - 2
LA  - en
AB  - The aim of this study was to determine the levels of Cd, Co, Cr, Fe, Mn, Mn, Pb, Ni, Zn and hepatic metallothionein (MT) in muscle, liver, kidney and gills of Capoeta trutta and Capoeta umbla caught from the Murat River. The length and weight of the fish were measured. The physical and chemical properties of the river water were measured and compared with the tolerable limits of Turkish Standards Institute (TSE). The tissues to be analysed for heavy metals were prepared for the analyses using the wet incineration method in a closed system. The heavy metal content of the samples was measured by inductively coupled plasma optical emission spectroscopy (ICP-OES). Differences in heavy metal accumulation of Capoeta trutta and Capoeta umbla in different tissues and different metal species were determined. The levels of Cr, Mn, Ni and Fe in the muscle tissues of Capoeta trutta were higher than the limits recommended for fish by the Turkish Food Codex (TGK), the Food and Agriculture Organization (FAO) and the World Health Organisation (WHO). Muscle Cr, Mn, Ni and Fe levels of Capoeta umbla were higher than the tolerable levels recommended by FAO and WHO. Heavy metals cannot be metabolised in tissues. They tend to accumulate. It has been observed that this accumulation varies in muscle, liver, kidney and gills according to organ structure, metabolic activity and defence mechanisms against toxic substances. MT, an indicator of tissue metal accumulation, was found to differ between Capoeta trutta and Capoeta umbla liver tissues.
KW  - Capoeta trutta
KW  - Capoeta umbla
KW  - Heavy metal
KW  - Metallothionein
KW  - Murat River
N2  - Bu çalışmanın amacı, Murat Nehri'nde yaşayan Capoeta trutta ve Capoeta umbla balıklarının kas, karaciğer, böbrek ve solungaçlarındaki Cd, Co, Cr, Fe, Mn, Pb, Ni, Zn ve karaciğer metallothionein (MT) seviyelerini belirlemektir. Balıkların boy ve ağırlıkları ölçülmüştür. Nehir suyunun fiziksel ve kimyasal özellikleri ölçülmüş ve Türk Standartları Enstitüsü'nün (TSE) tolere edilebilir limitleri ile karşılaştırılmıştır.  Ağır metal analizi yapılacak dokular kapalı sistem yaş yakma metodu kullanılarak analizlere hazır hale getirildi. Örneklerin ağır metal içeriği indüktif eşleşmiş plazma optik emisyon spektroskopisi (ICP-OES) ile ölçülmüştür. Capoeta trutta ve Capoeta umbla'nın farklı dokularında ve farklı metal türlerinde ağır metal birikimindeki farklılıklar belirlenmiştir. Capoeta trutta'nın kas dokularındaki Cr, Mn, Ni ve Fe seviyeleri, Türk Gıda Kodeksi (TGK), Birleşmiş Milletler Gıda ve Tarım Örgütü (FAO) ve Dünya Sağlık Örgütü (WHO) tarafından balıklar için önerilen limitlerden daha yüksek ölçüldü. Capoeta umbla'nın kas Cr, Mn, Ni ve Fe seviyeleri FAO ve WHO tarafından önerilen tolere edilebilir seviyelerden daha yükse bulundu. Ağır metaller dokularda metabolize edilemezler. Birikme eğilimindedirler. Bu birikimin kas, karaciğer, böbrek ve solungaçlarda organ yapısı, metabolik aktivite ve toksik maddelere karşı savunma mekanizmalarına göre değiştiği gözlemlenmiştir. Dokulardaki metal birikiminin bir göstergesi olan MT seviyesi Capoeta trutta ve Capoeta umbla karaciğer dokusunda farklı bulunmuştur.
CR  - Abass, N. Y. (2024). The influence of heavy metals on cytotoxicity in Tilapia zillii. Aquaculture International, 32, 9079-9098. https://doi.org/10.1007/s10499-024-01604-2
CR  - Akbulut, N. E., & Tuncer, A. M. (2011). Accumulation of heavy metals with water quality parameters in Kızılırmak River Basin (Delice River) in Turkey. Environmental Monitoring and Assessment, 173(1–4), 387–395. https://doi.org/10.1007/s10661-010-1394-7
CR  - Al-Jobory, A. A. S., & Yucel, U. M. (2019). Analysis of some heavy metals accumulation in water, sediment and tissues of different fish species from the razzaza lake, Iraq. Fresenius Environ Bullet, 28(7), 5041–5051.
CR  - Allen-Gil, S. M., & Martynov, V. G. (1995). Heavy metal burdens in nine species of freshwater and anadromous fish from the Pechora River, northern Russia. Science of the Total Environment, 160–161(C), 653–659. https://doi.org/10.1016/0048-9697(95)93634-T
CR  - Anan, Y., Kunito, T., Tanabe, S., Mitrofanov, I., & Aubrey, D. G. (2005). Trace element accumulation in fishes collected from coastal waters of the Caspian Sea. Marine Pollution Bulletin, 51(8-12), 882-888. https://doi.org/10.1016/j.marpolbul.2005.06.038
CR  - Atici, T., Obali, O., Altindag, A., Ahiska, S., & Aydin, D. (2010). The accumulation of heavy metals (Cd, Pb, Hg, Cr) and their state in phytoplanktonic algae and zooplanktonic organisms in Beysehir Lake and Mogan Lake, Turkey. African Journal of Biotechnology, 9(4).
CR  - Bayhan, T., & Ünübol Aypak, S. (2016). Büyük Menderes Deltasindan avlanan Kefal ve Levreklerde Cu, Zn ve Cd düzeylerinin belirlenmesi ve Metallotiyonin ile ilişkisinin araştirilmasi. Gida / The Journal Of Food, 5, 359–365.
CR  - Canli, M., & Atli, G. (2003). The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environmental Pollution, 121(1), 129–136. https://doi.org/10.1016/S0269-7491(02)00194-X
CR  - Coetzee, L., Du Preez, H. H., & Van Vuren, J. H. J. (2002) Metal concentrations in Clarias gariepinus and Labeo umbratus from the Olifants and Klein Olifants River, Mpumalanga, South Africa: Zinc, copper, manganese, lead, chromium, nickel, aluminium and iron. Water SA, 28(4), 433–448. https://doi.org/10.4314/wsa.v28i4.4917
CR  - Cordeli, A. N., Oprea, L., Crețu, M., Dediu, L., Coadă, M. T., & Mînzală, D. N. (2023). Bioaccumulation of metals in some fish species from the Romanian Danube River: A review. Fishes, 8(8), 387. https://doi.org/10.3390/fishes8080387
CR  - Cucu, A. K., Topkaya, M., Erdogan, G., & Aboul-Enein, H. Y. (2019). Quantitative determination of heavy metal contamination in horse mackerel and whiting caught in the sea of Marmara. Bulletin of Environmental Contamination and Toxicology, 102, 498-503. https://doi.org/10.1007/s00128-019-02574-5
CR  - Çiftçi, H., Çalışkan, Ç. E., & Öztürk, K. (2021). Bazı Balık Türlerinde İz ve Toksik Metal Düzeylerinin Belirlenmesi ve İnsan Sağlığı Riskinin Değerlendirilmesi. Journal of Advanced Research in Natural and Applied Sciences, 7(2), 219-233. https://doi.org/10.28979/jarnas.883611
CR  - Demirezen, D., & Aksoy, A. (2005). Determination of heavy metals in bee honey using by inductively coupled plasma optical emission spectrometry (ICP-OES). Gazi University Journal of Science, 18(4), 569-575.
CR  - Díez, S., Delgado, S., Aguilera, I., Astray, J., Pérez-Gómez, B., Torrent, M., & Bayona, J. M. (2009). Prenatal and early childhood exposure to mercury and methylmercury in Spain, a high-fish-consumer country. Archives of Environmental Contamination and Toxicology, 56, 615-622. https://doi.org/10.1007/s00244-008-9213-7
CR  - Dindaş, F., & Emre, Y. (2020). Kırkgöz su kaynakları ve Çırnık Köprüsü arasında yaşayan Pseudophoxinus antalyae, Bogutskaya, 1992’nın kas dokusunda bazı ağır metal düzeylerinin araştırılması. Journal of Limnology and Freshwater Fisheries Research, 6(3), 261–269. https://doi.org/10.17216/limnofish.709801
CR  - Dong, W. Q. Y., Cui, Y., & Liu, X. (2001). Instances of soil and crop heavy metal contamination in China. Soil and Sediment Contamination, 10(5), 497-510. https://doi.org/10.1080/20015891109392
CR  - EC. (1998). On the quality of water intended for human consumption. Official Journal of the European Communities, 330, 32–54. Accessed: 21 January 2025. https://aei.pitt.edu/10298/1/10298.pdf
CR  - Egemen, Ö. (1999). Çevre ve su kirliliği, Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, 42, 101.
CR  - EPA. (2007). Drinking water (No.2) regulations. https://www.epa.ie/publications/compliance--enforcement/drinking-water/advice--guidance/epa-private-water-supplies-handbook.pdf
CR  - Erdoǧrul, Ö., & Erbilir, F. (2007). Heavy metal and trace elements in various fish samples from Sir Dam Lake, Kahramanmaraş, Turkey. Environmental Monitoring and Assessment, 130(1–3), 373–379. https://doi.org/10.1007/s10661-006-9404-5
CR  - Eroğlu, M., & Düşükcan, M. (2016). Some heavy metals in the muscle of Capoeta trutta: risk assessment for the consumers. Cellular and Molecular Biology, 62(6), 22–26.
CR  - Erol, O. (1993). Türkiye’nin doğal yöre ve çevreleri. Ege Coğrafya Dergisi, 7(1), 13-41.
CR  - FAO. (1983). Compilation of legal limits for hazardous substances in fish and fishery products. Fish Circular, 464, 5-100.
CR  - FAO/WHO. (1989). Evaluation of certain food additives and the contaminants mercury, lead and cadmium. WHO Technical Report Series No. 505.
CR  - Fırat, Ö., Fırat, Ö., Çoğun, H. Y., Aytekin, T., Fridin, G., Temiz, Ö., & Kargın, F. (2018). Atatürk Baraj Gölü’nün kirli ve temiz bölgelerinden yakalanan balıkların (Silurus triostegus Heckel, 1843, Chalcalburnus tarichi Pallas, 1811, Chondrostoma regium Heckel, 1843, Carassius carassius Linnaeus, 1758) dokularındaki ağır metal düzeylerinin karşılaştırılması. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 14(3), 173-183. https://doi.org/10.22392/egirdir.375767
CR  - Fujita, H., Nishitani, C., & Ogawa, K. (2002). Lead, chemical porphyria, and heme as a biological mediatör. The Tohoku Journal of Experimental Medicine, 196(2), 53–64. https://doi.org/10.1620/tjem.196.53
CR  - George, S. (1991). Cell Biochemistry and Transmembrane Transport of Some Metals, in E. Merian (eds.). Metals and their compounds in the environment: Occurrence, analysis and biological relevance, (pp. 511–521) VCH Publishers.
CR  - Gül, A., Türk, C., İspir, Ü., Kırıcı, M., Taysı, M. R., & Yonar, M. E. (2017). Murat Nehri’nde (Genç-Bingöl) avlanan bazı Cyprinid’lerde Neoechinorhynchus rutili (Müller, 1780) (Acanthocephala)’nin araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 14(3), 163-168.
CR  - Gümüş, N. E., & Akköz, C. (2020). Eber Gölü (Afyonkarahisar) su kalitesinin araştırılması. Journal of Limnology and Freshwater Fisheries Research, 6(2), 153–163. https://doi.org/10.17216/limnofish.638567
CR  - Güneş, M., Sökmen, T., & Kirici, M. (2019). Determination of some metal levels in water, sediment and fish species of tercan dam lake, Turkey. Applied Ecology and Environmental Research, 17(6), 14961–14972. https://doi.org/10.15666/aeer/1706_1496114972
CR  - Heath, A. G. (2018). Water pollution and fish physiology, Second Edition. Florida USA: CRC Press. https://doi.org/10.1201/9780203718896
CR  - Hogstrand, C., & Haux, C. (1990). Metallothionein as an indicator of heavy-metal exposure in two subtropical fish species. Journal of Experimental Marine Biology and Ecology, 138(1–2), 69–84. https://doi.org/10.1016/0022-0981(90)90177-E
CR  - Honarmand, M., Khajehpour, S., Shahriari, H., & Hoseinjani, M. (2017). Co-contamination of arsenic and other trace elements (Hg, Pb, Al, Fe, Cr, Ni, and Cd) in the Rafsanjan plain alluvial aquifer se of iran and arsenic risk assessment. Open Journal of Geology, 7(11), 1710-1723. https://doi.org/10.4236/ojg.2017.711114
CR  - İsanç, A. T. (2010). Farklı baraj göllerindeki balıkların ağır metal birikimleri üzerine araştırmalar. Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü, Afyon.
CR  - Järup, L. (2003). Hazards of heavy metal contamination. British Medical Bulletin, 68, 167–182. https://doi.org/10.1093/bmb/ldg032
CR  - Javed, M., & Usmani, N. (2011). Accumulation of heavy metals in fishes: A human health concern. International Journal of Environmental Sciences, 2(2), 659–670.
CR  - Javed, M., & Usmani, N. (2013). Assessment of heavy metal (Cu, Ni, Fe, Co, Mn, Cr, Zn) pollution in effluent dominated rivulet water and their effect on glycogen metabolism and histology of Mastacembelus armatus. SpringerPlus, 2(1), 1–13. https://doi.org/10.1186/2193-1801-2-390
CR  - Kacar, E., Akin, H. K., Cicek, T., & Unlu, E. (2017). Seasonal variations of heavy metals in water, sediment and tissues of two freshwater fish species (Cyprinion macrostomum and Capoeta trutta) from the Batman Dam (Tigris Basin), Turkey. Fresenius Environmental Bullet, 26(6), 4187-4193.
CR  - Kägi, J. H. R. (1991). Overview of metallothionein. Methods in Enzymology, 205(C), 613–626. https://doi.org/10.1016/0076-6879(91)05145-L
CR  - Karayakar, F., Işık, U., Cicik, B., & Canli, M. (2022). Heavy metal levels in economically important fish species sold by fishermen in Karatas (Adana/TURKEY). Journal of Food Composition and Analysis, 106, 104348. https://doi.org/10.1016/j.jfca.2021.104348
CR  - Karaytug, S., Erdem, C., & Cicik, B. (2007). Accumulation of cadmium in the gill, liver, kidney, spleen, muscle and brain tissues of Cyprinus carpio. Ekoloji, 16(63), 16–22.
CR  - Kırıcı, M., Taysı, M. R., Bengü, A. Ş., & İspir, Ü. (2013). Murat Nehri’nden yakalanan Capoeta capoeta umbla (Heckel, 1843)’da bazı metal düzeylerinin belirlenmesi. Journal of the Institute of Science and Technology, 3(1), 85-90.
CR  - Küçük, S. (2018). Su ürünlerinde metallotionein proteinleri. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 15(2), 107–112. https://doi.org/10.25308/aduziraat.427601
CR  - Lawson, O. E. (2011). Physico-chemical parameters and heavy metal contents of water from the Mangrove Swamps of Lagos Lagoon, Lagos, Nigeria. Advances in Biological Research, 5(1), 8–21.
CR  - Mert, R., Bulut, S., & Solak, K. (2008). Apa Baraj Gölü’nün (Konya) bazı fiziksel ve kimyasal özelliklerinin araştırılması. Fen ve Mühendislik Bilimleri Dergisi, 8(2), 1–10.
CR  - Mijošek, T., Marijić, V. F., Dragun, Z., Ivanković, D., Krasnići, N., Erk, M., & Kepčija, R. M. (2019). Comparison of electrochemically determined metallothionein concentrations in wild freshwater salmon fish and gammarids and their relation to total and cytosolic metal levels. Ecological Indicators, 105, 188-198. https://doi.org/10.1016/j.ecolind.2019.05.069
CR  - Mol, S., Özden, Ö., & Oymak, S.A. (2010). Trace metal contents in fish species from ataturk dam lake (Euphrates, Turkey). Turkish Journal of Fisheries and Aquatic Sciences, 10(2), 209–213. https://doi.org/10.4194/trjfas.2010.0208
CR  - Murugan, S. S., Karuppasamy, R., Poongodi, K., & Puvaneswari, S. (2008). Bioaccumulation pattern of zinc in freshwater fish Channa punctatus (Bloch.) after chronic exposure. Turkish Journal of Fisheries and Aquatic Sciences, 8(1), 55-59.
CR  - Mutluay, H., & Demirak, A. (1996). Su kimyası. İstanbul: Beta Basım Yayım Dağıtım.
CR  - Noori, R., Berndtsson, R., Hosseinzadeh, M., Adamowski, J. F., & Abyaneh, M. R. (2019). A critical review on the application of the National Sanitation Foundation Water Quality Index. Environmental Pollution, 244, 575-587. https://doi.org/10.1016/j.envpol.2018.10.076
CR  - Nriagu, J. O., & Pacyna, J. M. (1988). Quantitative assessment of worldwide contamination of air, water and soils by trace metals. Nature, 333(6169), 134–139. https://doi.org/10.1038/333134A0
CR  - Osman, A. G. M., & Kloas, W. (2010). Water quality and heavy metal monitoring in water, sediments, and tissues of the African Catfish Clarias gariepinus (Burchell, 1822) from the River Nile, Egypt. Journal of Environmental Protection, 01(04), 389–400. https://doi.org/10.4236/jep.2010.14045
CR  - Özan, C. (2016). Isparta Deresi'nin su ve sedimentindeki ağır metal birikiminin incelenmesi. (Master's Thesis), Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Isparta.
CR  - Özgür, C., & Çalta, M. (2003). Heavy metals in some tissues and organs of Capoeta capoeta umbla (Heckel, 1843) fish species in relation to body size, age, sex and seasons. Fresenius Environmental Bulletin, 12(9), 961–966.
CR  - Qu, C. S., Ma, Z. W., Yang, J., Liu, Y., Bi, J., & Huang, L. (2012). Human exposure pathways of heavy metals in a lead-zinc mining area, Jiangsu Province, China. PloS one, 7(11), e46793. https://doi.org/10.1371/journal.pone.0046793
CR  - Shovon, M. N. H., Majumdar, B. C., & Rahman, Z. (2017). Heavy metals (lead, cadmium and nickel) concentration in different organs of three commonly consumed fishes in Bangladesh. Fisheries and Aquaculture Journal, 8(3), 1-6. https://doi.org/10.4172/2150-3508.1000207
CR  - Sökmen, T. Ö., Güneş, M., & Kırıcı, M. (2018). Karasu Nehri’nden (Erzincan) alınan su, sediment ve Capoeta umbla dokularındaki ağır metal düzeylerinin belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 5(4), 578–588. https://doi.org/10.30910/turkjans.471355
CR  - Sönmez, A. Y., Kadak, A. E., Özdemir, R. C., & Bilen, S. (2016). Establishing on heavy metal accumulation in some economically important fish species captured from Kastamonu costal. Alinteri Journal of Agriculture Science, 31(2), 84-90.
CR  - Tanır, Ö. Z. (2021). Determination of heavy metals in some tissues of four fish species from the Karasu River (Erzincan, Turkey) for public consumption. Oceanological and Hydrobiological Studies, 50(2), 232–246. https://doi.org/10.2478/oandhs-2021-0020
CR  - Tekin-Özan, S., & Kir, I. (2005). Comparative study on the accumulation of heavy metals in different organs of tench (Tinca tinca L. 1758) and plerocercoids of its endoparasite Ligula intestinalis. Parasitology Research, 97(2), 156–159. https://doi.org/10.1007/s00436-005-1412-9
CR  - TGK. (2011). Türk gıda kodeksi bulaşanlar yönetmeliği, 29.12.2011 Tarih ve 28157 Sayılı 3 üncü mükerrer Resmi Gazete. Ankara. https://www.resmigazete.gov.tr/eskiler/2011/12/20111229m3-8.htm
CR  - TSE. (2005). İnsani tüketim amaçlı sular hakkında yönetmelik, 17.02.2005 tarih ve 25730 sayılı Resmi Gazete. https://www.resmigazete.gov.tr/eskiler/2005/02/20050217-3.htm
CR  - Uslu, H. (2007). Kars Çayı’ndan avlanan Siraz (Capoeta capoeta capoeta Guldenstaedt, 1772) balıklarında ve ortam sedimentinde bazı ağır metallerin derişim düzeylerinin incelenmesi. (Master's Thesis), Kafkas University, Fen Bilimleri Enstitüsü, Kars.
CR  - Uslu, O., & Türkman, A. (1987). Su Kirliliği ve Kontrolü, TC Başbakanlık Çevre Genel Müdürlüğü Yayınları. Eğitim Dizisi, 1, p. 364. https://kutuphane.tarimorman.gov.tr/vufind/Record/4967
CR  - Vallee, B. L. (1995). The function of metallothionein Neurochemistry International, 27(1), 23–33. https://doi.org/10.1016/0197-0186(94)00165-Q
CR  - Van Campenhout, K., Bervoets, L., & Blust, R. (2003). Metallothionein concentrations in natural populations of gudgeon (Gobio gobio): Relationship with metal concentrations in tissues and environment. Environ Toxicol Chemistry, 22(7), 1548–1555. https://doi.org/10.1002/etc.5620220717
CR  - Varol, M., & Tokatlı, C. (2023). Evaluation of the water quality of a highly polluted stream with water quality indices and health risk assessment methods. Chemosphere, 311, 137096. https://doi.org/10.1016/j.chemosphere.2022.137096
CR  - Varol, M., Kaçar, E., & Akın, H. K. (2020). Accumulation of trace elements in muscle, gill and liver of fish species (Capoeta umbla and Luciobarbus mystaceus) in the Tigris River (Turkey), and health risk assessment. Environmental Research, 186, 109570. https://doi.org/10.1016/j.envres.2020.109570
CR  - Wang, H., & Stuanes, A. O. (2003). Heavy metal pollution in air-water-soil-plant system of Zhuzhou City, Hunan Province, China. Water, Air, and Soil Pollution, 147, 79–107. https://doi.org/10.1023/A:1024522111341
CR  - WHO. (‎1993)‎. Guidelines for drinking-water quality: volume 1: recommendations, 2nd ed. World Health Organization. https://iris.who.int/handle/10665/259956
CR  - WHO. (2002). Guidelines for drinking-water quality. World Health Organization. https://www.ircwash.org/sites/default/files/243-02WH-17308.pdf
CR  - Wong, S. L., & Lye, E. J. (2008). Lead, mercury and cadmium levels in Canadians. Health Reports, 19(4).
CR  - Yeşilbudak, B., & Erdem, C. (2014). Cadmium accumulation in gill, liver, kidney and muscle tissues of common carp, Cyprinus carpio, and Nile tilapia, Oreochromis niloticus. Bulletin of Environmental Contamination and Toxicology, 92, 546-550. https://doi.org/10.1007/s00128-014-1228-3
UR  - https://doi.org/10.53433/yyufbed.1641740
L1  - https://dergipark.org.tr/en/download/article-file/4618096
ER  -