TY  - JOUR
T1  - LC–MS/MS Profiling of Algerian Zizyphus lotus Extracts and their in vitro Antioxidant Activity: A Comparative Study
TT  - Cezayir Zizyphus lotus Özütlerinin LC–MS/MS Profillemesi ve Bunların in vitro Antioksidan Aktivitesi: Karşılaştırmalı Bir Çalışma
AU  - Bakchiche, Boulanouar
AU  - Hadjira, Guenane
AU  - Erenler, Ramazan
AU  - A. Ghareeb, Mosad
PY  - 2025
DA  - September
Y2  - 2025
DO  - 10.31594/commagene.1654147
JF  - Commagene Journal of Biology
JO  - Comm. J. Biol.
PB  - ABADER (Adıyaman Bilimsel Araştırmalar Derneği)
WT  - DergiPark
SN  - 2602-456X
SP  - 137
EP  - 144
VL  - 9
IS  - 2
LA  - en
AB  - In this study, it was aimed to determine antioxidant activities and chemical composition of leaf and fruit extracts of Zizyphus lotus which has been used traditionally in folk medicine of Algeria. Total phenolic content (TPC) and total flavonoid content (TFC) were determined by the colorimetric assay, respectively. The extracts were analyzed by LC–MS/MS. Furthermore, the antioxidant potential of various extracts was evaluated using different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS), and Phenanthroline activity using UV spectrophotometer. The quantitative analysis of polyphenols and flavonoids content showed that the leaves have the highest amount (279.07±18.84 mg GAE/g E and 132.74±27.74 mg ER/g E, respectively). The highest antioxidant activity was observed in the leaves with the value of 24.10±3.49 µg/mL, 9.365 ± 0.532 µg/mL, 0.19±0.093, 0.636±0.033 mg AAE/g, and 12.11±0.81 µg/mL for DPPH, ABTS, total antioxidant capacity, FRAP and Phenanthroline activity, respectively. The LC–MS/MS analysis of leaves reveals the presence the major compound, rutin (24392.59 mg/g), when fruits contain hesperidin (311.70 mg/g) as a major compound.
KW  - Zizyphus lotus
KW  - Total phenolic
KW  - Antioxidant potential
KW  - LC–MS/MS analysis
N2  - Bu çalışmada, Cezayir'de halk tıbbında geleneksel olarak kullanılan Zizyphus lotus'un yaprak ve meyve ekstraktlarının antioksidan aktivitelerinin ve kimyasal bileşiminin belirlenmesi amaçlanmıştır. Toplam fenolik içeriği (TPC) ve toplam flavonoid içeriği (TFC) sırasıyla kolorimetrik test ile belirlenmiştir. Ekstraktlar LC-MS/MS ile analiz edilmiştir. Ayrıca, çeşitli ekstraktların antioksidan potansiyeli, 2,2-difenil-1-pikrilhidrazil (DPPH) deneyi, ferrik indirgeyici antioksidan güç (FRAP) deneyi ve 2,2-azinobis-etilbenzotiyozolin-6-sülfonik asit (ABTS) ve Fenantrolin aktivitesi gibi farklı antioksidan deneyler kullanılarak UV spektrofotometresi kullanılarak değerlendirilmiştir. Polifenol ve flavonoid içeriğinin kantitatif analizi yaprakların en yüksek miktarda olduğunu göstermiştir (sırasıyla 279.07±18.84 mg GAE/g E ve 132.74±27.74 mg ER/g E). En yüksek antioksidan aktivite 24.10±3.49 µg/mL, 9.365 ± 0.532 µg/mL, 0.19±0.093, 0.636±0.033 mg AAE/g ve 12.11±0. değeri ile yapraklarda gözlenmiştir. DPPH, ABTS, Toplam antioksidan kapasite, FRAP ve Fenantrolin aktivitesi için sırasıyla 81 µg /mL. Yaprakların LC-MS/MS analizi, meyveler ana bileşik olarak hesperidin (311.70 mg/g) içerirken, ana bileşik olan rutinin (24392.59 mg/g) varlığını ortaya koymaktadır.
CR  - Abdoul-Azize, S. (2016). Potential benefits of Zizyphus lotus L. bioactive compounds for nutrition and health. Journal of Nutrition and Metabolism, 2016, 2867470. https://doi.org/10.1155/2016/2867470
CR  - Ait Bouzid, H., Sakar, E.H., Bijla, L., Ibourki, M., Zeroual, A., Gagour, J., Koubachi, J., Majourhat, K., & Gharby, S. (2022). Physical fruit traits, proximate composition, antioxidant activity, and profiling of fatty acids and minerals of wild jujube (Zizyphus lotus L. (Desf.)) fruits from eleven Moroccan origins. Journal of Food Quality, 2022(1), 9362366. https://doi.org/10.1155/2022/9362366
CR  - Atalar, M.N., Erenler, R., Turkan, F., Alma, M.H., Demirtas, I., Baran, A., Irtegun Kandemir, S., Kekec, A.I., & Saltan, F.Z. (2023). Phytochemical analysis and biological activity of Corchorus olitorius L.: Quantitative analysis of bioactive compounds by LC–MS/MS, antibacterial, enzyme inhibition, and cytotoxic activities. European Journal of Integrative Medicine, 62, 102290. https://doi.org/10.1016/j.eujim.2023.102290
CR  - Bekkar, N.E.H., Meddah, B., Cakmak, Y.S., & Keskin, B. (2021). Phenolic composition, antioxidant, and antimicrobial activities of Zizyphus lotus L. and Ruta chalepensis L. growing in Mascara (Western Algeria). Journal of Microbiology, Biotechnology and Food Sciences, 10, 1–9. https://doi.org/10.15414/jmbfs.300
CR  - Benammar, C., Hichami, A., Yessoufou, A., Simonin, A.M., Belarbi, M., Allali, H., & Khan, N.A. (2010). Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation. BMC Complementary and Alternative Medicine, 10, 54. https://doi.org/10.1186/1472-6882-10-54
CR  - Bencheikh, N., Bouhrim, M., Alami Merrouni, I., Boutahiri, S., Kharchoufa, M., Addi, L., Tungmunnithum, D., Hano, C., Eto, B., Legssyer, A., & Elachouri, M. (2021). Antihyperlipidemic and antioxidant activities of flavonoid-rich extract of Zizyphus lotus (L.) Lam. fruits. Applied Sciences, 11, 7788. https://doi.org/10.3390/app11177788
CR  - Borgi, W., Bouraoui, A., & Chouchane, N. (2007). Antiulcerogenic activity of Zizyphus lotus (L.) extracts. Journal of Ethnopharmacology, 112, 228–231. https://doi.org/10.1016/j.jep.2007.02.024
CR  - Chanda, S., & Dave, R. (2009). In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research, 3, 981–996.
CR  - Chen, J., Liu, X., Li, Z., Qi, A., Yao, P., Zhou, Z., ... & Tsim, K. W. (2017). A review of dietary Ziziphus jujuba fruit (jujube): Developing health food supplements for brain protection. Evidence-Based Complementary and Alternative Medicine, 24, 110. https://doi.org/10.1155/2017/3019568
CR  - Dahlia, F., Barouagui, S., Hemida, H., Bousaadia, D., & Rahmoune, B. (2020). Influence of environmental variations on anti-glycaemic, anti-cholesterolemic, antioxidant, and antimicrobial activities of natural wild fruits of Ziziphus lotus (L.). South African Journal of Botany, 132, 215–225. https://doi.org/10.1016/j.sajb.2020.04.033
CR  - Dhibi, M., Amri, Z., Mnari Bhouri, A., Hammami, S., & Hammami, M. (2022). Comparative study of the phenolic profile and antioxidant activities of Moringa oleifera Lam. and Ziziphus lotus Linn. leaf extracts and their protective effects in frying stability of corn oil. Measurement: Food, 7, 100045. https://doi.org/10.1016/j.meafoo.2022.100045
CR  - El Atki, Y., Aouam, I., El Kamari, F., Taroq, A., Nayme, K., Timinouni, M., Lyoussi, B., & Abdellaoui, A. (2019). Phytochemistry, antioxidant, and antibacterial activities of two Moroccan Teucrium polium L. subspecies: Preventive approach against nosocomial infections. Journal of Advanced Pharmaceutical Technology & Research, 10(2), 63–67. https://doi.org/10.1016/j.arabjc.2019.04.001
CR  - Cadi, H.E., Bouzidi, H. E., Selama, G., Cadi, A. E., Ramdan, B., Oulad El Majdoub, Y., ... & Cacciola, F. (2020). Physico-chemical and phytochemical characterization of Moroccan wild jujube Zizyphus lotus (L.) fruit crude extract and fractions. Molecules, 25, 5237. https://doi.org/10.3390/molecules25225237
CR  - El Maaiden, E., El Kharrassi, Y., Lamaoui, M., Allai, L., Essamadi, A.K., Nasser, B., & Moustaid, K. (2020). Variation in minerals, polyphenolics, and antioxidant activity of pulp, seed, and almond of different Ziziphus species grown in Morocco. Brazilian Journal of Food Technology, 23, e2019206. https://doi.org/10.1590/1981-6723.20619
CR  - Elaloui, M., Ennajah, A., Ghazghazi, H., Youssef, I.B., Othman, N.B., Hajlaoui, M.R., ... & Laamouri, A. (2017). Quantification of total phenols, flavonoids, and tannins from Ziziphus jujuba (Mill.) and Ziziphus lotus (L.) (Desf.) leaf extracts and their effects on antioxidant and antibacterial activities. International Journal of Secondary Metabolites, 4, 18–26. https://doi.org/10.21448/ijsm.275886
CR  - Elaloui, M., Ammari, Y., Ghazghazi, H., Ben Othmen Gorrab, A., Laamouri, A., & Driouich Chaouachi, R. (2023). Effect on Ziziphus jujuba Mill. fruit powders embedded on physicochemical properties, biological activities, and rheologic quality of cake. Food Science & Nutrition, 11, 2942–2955. https://doi.org/10.1002/fsn3.3276
CR  - Gao, Q.H., Wu, C.S., Yu, J.G., Wang, M., Ma, Y.J., & Li, C.L. (2012). Textural characteristic, antioxidant activity, sugar, organic acid, and phenolic profiles of 10 promising jujube (Ziziphus jujuba Mill.) selections. Journal of Food Science, 77, 1218–1225. https://doi.org/10.1111/j.1750-3841.2012.02946.x
CR  - Ghedira, K., Chemli, R., Richard, B., Nuzillard, J.M., Zeches, M., & Men-Olivier, L. (1993). Two cyclopeptide alkaloids from Zizyphus lotus. Phytochemistry, 32, 1591–1594.
CR  - Ghedira, K. (2013). Zizyphus lotus (L.) Desf. (Rhamnaceae): Jujubier sauvage. Phytothérapie, 11, 149–153. https://doi.org/10.1007/s10298-013-0776-8
CR  - Guirado, E., Tabik, S., Alcaraz-Segura, D., Cabello, J., & Herrera, F. (2017). Deep-learning Versus OBIA for scattered shrub detection with Google Earth Imagery: Ziziphus lotus as case study. Remote Sensing, 9, 1220. https://doi.org/10.3390/rs9121220
CR  - Guenane, H., Bakchiche, B., Erenler, R., Yildiz, I., Mohamed, A.S., & El-Shazly, M.A.M. (2024). Mineral contents, antioxidant and antimicrobial activities of Algerian Terfezia claveryi extracts. FABAD Journal of Pharmaceutical Sciences, 49(1), 19–36. https://doi.org/10.55262/fabadeczacilik.1326710
CR  - Hammi, K.M., Jdey, A., Abdelly, C., Majdoub, H., & Ksouri, R. (2015). Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology. Food Chemistry, 184, 80–89. https://doi.org/10.1016/j.foodchem.2015.03.047
CR  - Hossain, A., Hamood, A., & Humaid, M.T. (2016). Comparative evaluation of total phenols, flavonoids content and antioxidant potential of leaf and fruit extracts of Omani Ziziphus jujuba L. Pacific Science Review A: Natural Science and Engineering, 18, 78–83. https://doi.org/10.1016/j.psra.2016.09.001
CR  - Jae Young, J., Jong Hoon, A., Yang Hee, J., Bang Yeon, H., & Mi Kyeong, L. (2019). Antioxidant Activity and Phenolic Content of Different Parts of Lotus and Optimization of Extraction Condition using Response Surface Methodology. Natural Product Sciences, 25(1), 44-48. https://doi.org/10.20307/nps.2019.25.1.44
CR  - Khouchlaa, A., Talbaoui, A., El Yahyaoui El Idrissi, A., Bouyahya, A., Ait Lahsen, S., Kahouadji, A., & Tijane, M. (2020). Determination of phenol content and evaluation of in vitro litholytic effects on urolithiasis of Moroccan Zizyphus lotus L. extract. Phytotherapie, 16, 1. https://doi.org/10.1007/s10298-017-1106-3
CR  - Kuhkheil, A., Naghdi Badi, H., Mehrafarin, A., & Abdossi, V. (2020). Phytochemical and morpho-physiological variations in sea buckthorn (Hippophae rhamnoides L.) populations of Taleghan region in Iran. Journal of. Medicinal Plants, 21–35. https://doi.org/10.29252/jmp.19.76.21
CR  - Lahlou, M., & Mahi, J.H. (2002). Évaluation des activités antifongique et molluscicide de Zizyphus lotus (L.) Desf. du Maroc. Annales Pharmaceutiques Françaises, 60, 410–414.
CR  - Letaief, T., Garzoli, S., Masci, V.L., Mejri, J., Abderrabba, M., Tiezzi, & Ovidi, E. (2021). Chemical composition and biological activities of Tunisian Ziziphus lotus extracts: Evaluation of drying effect, solvent extraction, and extracted plant parts. Plants (Basel), 10(12), 2651. https://doi.org/10.3390/plants10122651
CR  - Maraghni, M., Gorai, M., & Neffati, M. (2010). Seed germination at different temperatures and water stress levels, and seedling emergence from different depths of Ziziphus lotus. South African Journal of Botany, 76, 453–459.
CR  - Maraghni, M., Gorai, M., & Neffati, M. (2011). The influence of water-deficit stress on growth, water relations and solute accumulation in wild Jujube. Journal of Ornamental and Horticultural Plants, 1, 63–72. https://doi.org/10.1016/j.sajb.2010.02.092
CR  - Marmouzi, I., Kharbach, M., El Jemli, M., Bouyahya, A., Cherrah, Y., Bouklouze, A., Vander Heyden, Y., & Faouzi, M. (2019). Antidiabetic, dermatoprotective, antioxidant and chemical functionalities in Zizyphus lotus leaves and fruits. Industrial Crops and Products, 132, 134–139. https://doi.org/10.1016/j.indcrop.2019.02.007
CR  - Mkadmini Hammi, K., Jellouli Ennigrou, D., Majdoub, H., & Ksouri, R. (2017). Recovery of phenolic compounds and carbohydrates from hydro-ethanolic extract of Zizyphus lotus fruit using ultrafiltration process. International Journal of Food Engineering, 13, 1–13. https://doi.org/10.1515/ijfe-2017-0343
CR  - Prasad, K. N., Yang, B., Yang, S., Chen, Y., Zhao, M., Ashraf, M., & Jiang, Y. (2009). Identification of phenolic compounds and appraisal of antioxidant and antityrosinase activities from litchi (Litchi sinensis Sonn.) seeds. Food Chemistry, 116, 1–7. https://doi.org/10.1016/j.foodchem.2009.01.079
CR  - Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269, 337–41. https://doi.org/10.1006/abio.1999.4019
CR  - Saeed, N., Khan, R., & Shabbir, M.M. (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine, 12, 221. https://doi.org/10.1186/1472-6882-12-221
CR  - San, B., Yildirim, A.N., Polat, M., & Yildirim, F. (2009). Mineral composition of leaves and fruits of some promising jujube (Zizyphus jujuba Miller) genotypes. Asian Journal of Chemistry, 21, 2898–2902.
CR  - Saravanakumar, K., Sarikurkcu, C., Sahinler, S.S., Sarikurkcu, R.B., & Wang, M. (2021). Phytochemical Composition, Antioxidant, and Enzyme Inhibition Activities of Methanolic Extracts of Two Endemic Onosma Species. Plants, 10, 1373. https://doi.org/10.3390/plants10071373
CR  - Sharifi-Rad, M., & Pohl, P. (2020). Synthesis of biogenic silver nanoparticles (AgCl-NPs) using a Pulicaria vulgaris Gaertn. aerial part extract and their application as antibacterial, antifungal, and antioxidant agents. Nanomaterials, 10, 638. https://doi.org/10.3390/nano10040638
CR  - Szydlowska-Czerniak, A., Dianoczki, C., Recseg, K., Karlovits, G., & Szlyk, E. (2008). Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta, 76(4), 899–905. https://doi.org/10.1016/j.talanta.2008.04.055
CR  - Tlili, N., Tir, M., Benlajnef, H., Khemiri, S., Mejri, H., Rejeb, S., & Khaldi, A. (2014). Variation in protein and oil content and fatty acid composition of Rhus tripartitum fruits collected at different maturity stages in different locations. Industrial Crops and Products, 59, 197–201. https://doi.org/10.1016/j.indcrop.2014.05.020
CR  - Umair Riaz, M., Ali Raza, M., Saeed, A., Ahmed, M., & Tanveer, H. (2021). Variations in Morphological Characters and Antioxidant Potential of Different Plant Parts of Four Ziziphus Mill. Species from the Cholistan. Plants (Basel), 10(12), 2734. https://doi.org/10.3390/plants10122734
CR  - Yahia, Y., Benabderrahim, M.A., Tlili, N., Bagues, M., & Nagaz, K. (2020). Bioactive compounds, antioxidant and antimicrobial activities of extracts from different plant parts of two Ziziphus Mill. Species. PLoS One, 15(5), e0232599. https://doi.org/10.1371/journal.pone.0232599
UR  - https://doi.org/10.31594/commagene.1654147
L1  - https://dergipark.org.tr/en/download/article-file/4674083
ER  -