TY  - JOUR
T1  - Determination of Antioxidant and Anticancer Activities and Exopolysaccharides of Metabolites Synthesized by Probiotic Levilactobacillus brevis Strains Newly Isolated from Breast Milk
TT  - Anne Sütünden Yeni İzole Edilen Probiyotik Levilactobacillus brevis Suşlarının Sentezlediği Metabolitlerin Antioksidan ve Antikanser Aktiviteleri ile Ekzopolisakkarit Tayini
AU  - Ertaş, Metin
AU  - Alan, Yusuf
PY  - 2025
DA  - June
Y2  - 2025
DO  - 10.31594/commagene.1660124
JF  - Commagene Journal of Biology
JO  - Comm. J. Biol.
PB  - ABADER (Adıyaman Bilimsel Araştırmalar Derneği)
WT  - DergiPark
SN  - 2602-456X
SP  - 123
EP  - 132
VL  - 9
IS  - 2
LA  - en
AB  - In our study, the probiotic, exopolysaccharide, metabolite contents, antioxidant activities, and anticancer activities of metabolites on HT-29 and Caco-2 cell lines of four Levilactobacillus brevis (YAAS1, YAAS2, YAAS3, and YAAS4) strains isolated from breast milk in Türkiye and identified by MALDI-TOF MS were investigated. It was determined that the strains exhibited strong probiotic character by surviving in pH+pepsin, bile and pancreatin medium; although, they decreased depending on time and concentration. The strains synthesized the most acetic acid but could not synthesize tartaric, pyruvic, malic, and fumaric acids. It was determined qualitatively and quantitatively that the strains synthesized compounds similarly in terms of exopolysaccharide amount. The strains exhibited lower DPPH activity than standard antioxidants. However, ABTS activities were similar to standard antioxidants. Bacterial metabolites induced notable anticancer effects in the HT-29 cell line at low concentrations, while eliciting comparable effects in the Caco-2 cell line at high concentrations. When all the results were considered in general, it was determined that the strains showed identical properties to each other but L. brevis YAAS4 strain exhibited a better character structure. Given the scarcity of studies on Lactobacillus brevis strains newly isolated from human breast milk, this research significantly advances current understanding. Characterizing the specific properties identified in our study through future research will be crucial for determining the potential of these isolates as effective probiotics for industrial and health applications.
KW  - Levilactobacillus brevis
KW  - MALDI-TOF MS
KW  - HT-29
KW  - Caco-2
N2  - Çalışmamızda, Türkiye'deki anne sütünden yeni izole edilen ve MALDI-TOF MS ile tanımlanan dört Levilactobacillus brevis (YAAS1, YAAS2, YAAS3 ve YAAS4) suşunun probiyotik, ekzopolisakkarit, metabolit içerikleri, antioksidan aktiviteleri ve metabolitlerinin HT-29 ve Caco-2 hücre hatları üzerindeki antikanser aktiviteleri araştırıldı. Suşların pH+pepsin, safra ve pankreatin ortamında canlılığını sürdürerek güçlü probiyotik karakter sergilediği, ancak bu özelliğinin zamana ve konsantrasyona bağlı olarak azaldığı belirlendi. Suşlar en fazla asetik asit sentezlediği, ancak tartarik, pirüvik, malik ve fumarik asitleri sentezlemedikleri belirlendi. Ekzopolisakkarit miktarı bakımından suşlar birbirlerine benzer şekilde sentezlediği nitel ve nicel olarak belirlendi. Suşlar standart antioksidanlardan daha düşük DPPH aktivitesi gösterdi. Ancak ABTS aktiviteleri standart antioksidanlara benzerdi. Bakteriyel metabolitler düşük konsantrasyonlarda HT-29 hücre hattına karşı iyi antikanser etki gösterirken, yüksek konsantrasyonlarda Caco-2 hücre hattına karşı benzer etki gösterdi. Tüm sonuçlar genel olarak değerlendirildiğinde suşların birbirlerine benzer özellikler gösterdiği, ancak L. brevis YAAS4 suşunun daha iyi bir karakter yapısı sergilediği belirlendi. Sonuç olarak insan sütünden yeni izole edilen L. brevis suşları üzerinde sınırlı sayıda çalışma olması nedeniyle sonuçlarımız literatüre önemli katkılar sunacaktır. Aynı zamanda L. brevis suşlarımızın sergilediği özellikler dikkate alınarak yapılacak yeni araştırmalar ile iyi karakterdeki suşların endüstri ve sağlık alanında iyi bir probiyotik bakteri kaynağı olmasına katkı sağlayacaktır.
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UR  - https://doi.org/10.31594/commagene.1660124
L1  - https://dergipark.org.tr/en/download/article-file/4700711
ER  -