TY  - JOUR
T1  - Resveratrol’s Antiapoptotic and Antioxidant Role in Carbon Tetrachloride-Induced Nephrotoxicity of Rats
TT  - Ratlarda Karbon Tetraklorür ile Oluşturulan Nefrotoksisitede Resveratrol’ün Antiapoptotik ve Antioksidan Rolü
AU  - Başak, Feyza
AU  - Dinçel, Güngör Çağdaş
AU  - Orhan, Betül
AU  - Yıldırım, Serkan
PY  - 2025
DA  - July
Y2  - 2025
DO  - 10.36483/vanvetj.1661888
JF  - Van Veterinary Journal
JO  - Van Vet J
PB  - Van Yuzuncu Yıl University
WT  - DergiPark
SN  - 2149-3359
SP  - 126
EP  - 131
VL  - 36
IS  - 2
LA  - en
AB  - This study investigates the protective effect of Resveratrol on renal tissues utilizing histopathology, immunohistochemistry, and immunofluorescence methods in a carbon tetrachloride (CCl4)-induced nephrotoxicity model. Wistar albinos (male, n=32) were randomly selected and divided into groups. The initial control group, followed by the second group, received CCl4 (2mg/kg), the third group was administered Resveratrol (30 mg/kg/day), and the fourth group was co-administered with CCl4 and Resveratrol. After the experiment, rats were euthanized under anesthesia, and their kidneys were collected. Results revealed that in the CCl4 group, notable degeneration and necrosis of tubular epithelium, significant steatosis, and arterial hyperemia wereobserved. In the CCl4+Resveratrol group, renal tissues exhibited slight deterioration of tubular epithelium and hyperemia in the glomerular arteries. The immunohistochemistry approach employing 8- Hydroxy-deoxyguanosine (8-OHdG) was utilized to identify DNA damage in kidney tissue. Immunohistochemistry results indicated that both the control and resveratrol groups had a negative reaction for 8-OHdG, but the CCl4 group demonstrated a statistically significant increase in 8-OHdG expression in the tubular epithelium located cytoplasmically (p
KW  - Antioxidant
KW  - Caspase 3
KW  - CCl4
KW  - Resveratrol
KW  - 8-OHdG
N2  - Bu çalışma, karbon tetraklorür (CCl4) kaynaklı nefrotoksisite modelinde histopatoloji, immünohistokimya ve immünofloresan metotlarını kullanarak güçlü bir antioksidan olan Resveratrol'ün böbrek dokuları üzerindeki koruyucu etkisini incelemeyi amaçlamaktadır. Rastgele seçilen erkek Wistar sıçanları (n=32) dört gruba ayrıldı. Kontrol grubuna herhangi bir işlem uygulanmazken, ikinci gruba CCl4 (2 mg/kg), ve üçüncü gruba Resveratrol (30 mg/kg/gün) verildi ve dördüncü gruba CCl4 ve Resveratrol birlikte verildi. Deneyden sonra sıçanlara anestezi altında ötenazi uygulandı ve böbrek örnekleri toplandı. CCl4 grubunda histopatolojik olarak, tübüler epitelyumda belirgin dejenerasyon ve nekroz, hem glomerüler hem de interstisyel bölgede yer alan arterlerde belirgin steatoz ve hiperemi görüldü. CCl4+Resveratrol grubunda böbrek dokuları tübüler epitelde hafif bozulma ve glomerüler ve interstisyel alanların arterlerinde hiperemi gösterdi. Böbrek dokusunda DNA hasarını belirlemek için 8-OHdG antikoru ile immünohistokimyasal boyama yapıldı. İmmünohistokimya sonuçları hem kontrol hem de resveratrol gruplarında 8-OHdG antikoruna karşı bir reaksiyon oluşmadığını, ancak CCl4 grubuna ait tübüler epitelde sitoplazmik 8-OHdG ekspresyonunda istatistiksel olarak anlamlı bir artış görüldüğünü ortaya koydu (p
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UR  - https://doi.org/10.36483/vanvetj.1661888
L1  - https://dergipark.org.tr/en/download/article-file/4708762
ER  -