@article{article_1669161, title={Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells}, journal={Cukurova Medical Journal}, volume={50}, pages={752–760}, year={2025}, DOI={10.17826/cumj.1669161}, author={Batar, Bahadır and Kazancı, Mehmet and Karaoğlu, Ahmet Ensar and Genç, Alperen and Doğan, Furkan and Reyhan, Zeynel Utku and Çaba, Dilara Ceren and Günaydın, Yasemin and Baran, Muhammed and Mortaş, Eslem and et al.}, keywords={Asteraceae, breast cancer, Cirsium italicum, flavonoid, histone deacetylase, reactive oxygen species}, abstract={Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum (Savi) DC. plants in breast cancer cells. Materials and Methods: MDA-MB-231 triple-negative breast cancer (TNBC) cells were used to demonstrate reactive oxygen species (ROS) production effects and histone deacetylase (HDAC) inhibitory activity of flavonoids [5,3’,4’-trihydroxy-7-methoxy isoflavone (Santal) (1), 5,5’’,7’’-trihydroxy-3,7-dimethoxy-4’,4’’’-O-biflavone (2), 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside (3), (8,3’,4’,5’-tetrahydroxy-7-O-α-glycosyl isoflavone)-6-8’’-(4’’’-hydroxy-7’’-O-α-glycosyl isoflavone) biflavone (4)] isolated from C. italicum. Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mg/ml. Compound 2 at 0.06 mg/ml, and compounds 2 and 4 at 0.08 and 0.1 mg/ml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mg/ml, compound 2 showed a mean value of 0.003±0.004. At 0.08 mg/ml, the mean values were 0.006±0.018 for compound 2 and 0.013±0.013 for compound 4. At 0.1 mg/ml, compound 2 and compound 4 exhibited mean values of 0.014±0.017 and 0.014±0.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant. Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.}, number={3}, publisher={Cukurova University}