@article{article_1671304, title={Development and Validation of an UPLC-MS/MS Method for Quantification of Glyphosate in Urine}, journal={Fabad Eczacılık Bilimler Dergisi}, volume={50}, pages={395–404}, year={2025}, DOI={10.55262/fabadeczacilik.1671304}, author={Morgil, Göksel Koç and Çok, İsmet}, keywords={glyphosate, herbicide, urine, UPLC_MS/MS}, abstract={For the last four decades, the herbicide glyphosate has been the most widely used herbicide worldwide, including in Turkey, with the assumption that it has insignificant effects on the human and environmental health. However, particularly for the last decade, global concerns have escalated about the potential direct and indirect health risks it may pose to humans and ecosystems due to its high-volume use. Due to these increasing health concerns, especially cancer, the development of methods to detect traces of this herbicide in biological materials is of great importance for the protection of public health. To assess glyphosate exposure levels, it is crucial to have a selective, sensitive and precise analytical procedure that can determine low glyphosate concentrations, especially in complex biological matrices. In the presented study, we developed and validated an Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) procedure for the accurate and sensitive determination of glyphosate levels in urine. It is based on the “dilute and shoot” technique with no derivatization procedure and is quantitatively determined by UPLC-MS/MS. The advantages of this methodology are simplicity, minimal analyte loss, and high sample yield. The calibration curve for glyphosate was linear in the concentration range of 0.1–50 ng/mL. The limit of quantification (LOQ) for glyphosate was 0.1 ng/mL. This validated method can be applied very quickly and easily in the analysis of spot urine samples collected from healthy people.}, number={2}, publisher={FABAD Ankara Eczacılık Bilimleri Derneği}