@article{article_1671500, title={Comparison of Dental Follicle Stem Cells and Dental Pulp Stem Cells in a Translational Bone Tissue Engineering Protocol}, journal={EurAsian Journal of Oral and Maxillofacial Surgery}, volume={4}, pages={21–29}, year={2025}, author={Ozcan, Elif and Erbilgin, Yücel and Yıldırım, Selin and Zibandeh, Noushin and Akkoç, Tunç and Goker, Kamil}, keywords={Dental Follicle Stem Cell, Dental Pulp Stem Cell, Polycaprolactone, Tissue Engineering, Bone, Translational Medicine}, abstract={Purpose: In this study, it is aimed to establish and refine a translational protocol and compare the osteogenic potential of DPSC and DFSC’s on nano mesh containing (nmPCL) and plain (PCL) polycaprolactone scaffolds in vitro and contribute the translational medicine protocols in bone regeneration. Materials and Methods: DPSCs and DFSCs were osteogenically differentiated on PCL and nmPCL scaffolds and four groups were examined for cell proliferation and type I collagen formation rates after two weeks of culture. Following immunofluorescence labeling, Nonparametric (Kruskal Wallis) and multiple comparison tests were used to compare the four groups. Results: Among all groups, mean cell counts on scaffolds ranged from 30.8 to 82.6 cells/0.0915 mm2, and total collagen formation ranged from 2.79% to 17,9%. DFSC and nmPCL complex showed significantly higher cell counts (p < 0,01) and collagen formation rates (p < 0,01) in comparison to other groups. Conclusion: DFSC/nmPCL group is found to show superior properties on cell proliferation and bone matrix formation. This complex is a promising tool for maxillofacial tissue engineering applications.}, number={2}, publisher={Ağız ve Çene Yüz Cerrahisi Birliği Derneği}