@article{article_1677537, title={Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats}, journal={Ankara Üniversitesi Veteriner Fakültesi Dergisi}, pages={1–10}, year={2025}, DOI={10.33988/auvfd.1677537}, author={Deveci, Mehmet Zeki Yılmaz and Kırgız, Ömer and Özkan, Hüseyin and İşler, Cafer Tayer and Kazak Akçakavak, Filiz and Yurtal, Ziya and Yakan, Akın}, keywords={cineole, cutaneous, dermatology, experimental, topical}, abstract={A plant-derived monoterpene, eucalyptol (1,8-cineole), has been suggested to have antioxidant, anti-inflammatory, and antimicrobial properties. The wound-healing effects of eucalyptol remain unknown. This study aimed to evaluate the topical use of eucalyptol at two concentrations on a full-thickness excisional skin wound model in terms of wound healing, gene expression, biochemical changes, and histopathological changes. Sixty Wistar rats were randomly divided into 5 groups (n = 12 per group). The control group (C) was untreated. Full-thickness excisional skin wounds were created in the following groups: the vehicle group (V), which was treated with polysorbate 80 solution (negative control). The Dexpanthenol group (D) was treated with dexpanthenol ointment (positive control). Eucalyptol 5% (E5) and Eucalyptol 10% (E10) groups were treated with 5% and 10% eucalyptol, respectively. Wound areas were measured on days 1, 4, 7, 11, 14, 18, and 21 days after wound creation. Wound tissues were collected on days 7 and 21. Histopathological, gene expression (TNFα, IL10, TGFβ1, VEGF), and biochemical (MDA, rGSH, GPx, CAT) analyses were performed on the wound tissues. TNFα gene expression levels were upregulated in Groups V and E5 (P <0.05). IL10 levels were upregulated in the V, E5, and E10 groups (P <0.05). TGFβ1 was upregulated in all groups compared with the control. Significant differences in rGSH, GPx, CAT, and MDA levels were found among all groups (P <0.05). Topical administration of 10% eucalyptol accelerates wound healing. With respect to epidermal thickness, 5% eucalyptol resulted in superior wound healing. Further studies should include different wound models, tissue analyses, and combinations of eucalyptol with other agents.}, publisher={Ankara University}