TY - JOUR T1 - Analysis by Scanning Electron Microscopy of Polyethylene Terephthalate and Nylon Biodegradation Abilities of Bacillus sp. Strains Isolated From Soil AU - Demirkan, Elif AU - Güler, Baran Enes AU - Sevgi, Tuba PY - 2020 DA - December JF - Journal of Biological and Environmental Sciences JO - JBES PB - Bursa Uludağ University WT - DergiPark SN - 1307-9530 SP - 107 EP - 114 VL - 14 IS - 42 LA - en AB - Plastic pollution, the aggregation of synthetic plastic products in the environment generates serious problems for wildlife, habitats and the human population. Plastic accumulates at ocean, creating areas called the seventh continent, a mass of plastic garbage. Plastic wastes are disposed of by recycling, burying and incineration. However, these also have disadvantages. They are developing bioplastics as new solutions to plastic problems. Other alternative solutions may be microorganisms. Compared to other conventional technologies, it is extremely inexpensive and efficient in terms of cost and simplicity because it is based on the capabilities of microorganisms. In this study, we have reported the degradation abilities of B. subtilis and B. cereus strains on polyethylene terephthalate (PET, water bottle) and nylon (plastic bag). In the nylon medium with Bacillus subtilis ET18 and Bacillus cereus ET30 was observed red pigment formation. All bacteria showed biofilm formation in the presence of nylon. The surface morphology changes of the PET and nylon were determined by light microscope and SEM. The bacteria used in this study were found to biodegrade nylon more easily than PET. The activities of lipase, protease and α-amylase were determined. Among the enzymes, lipase was detected in the presence of both PET and nylon. KW - Bacillus KW - PET KW - nylon KW - biodegradation KW - SEM KW - enzyme CR - Bryant JA, Clemente TM., Viviani DA, Fong AA, Thomas KA, Kemp P, Karl DM., White AE, DeLong EF (2016) Diversity and activity of communities inhabiting plastic debris in the North Pacific Gyre. MSystems 1(3). CR - Bushnell LD, Haas HF (1941). The utilization of certain hydrocarbons by microorganisms. J. Bacterio., 41(5): 653-653. CR - Chaisu K (2016). Study of plastic degrading bacteria by Aneurinibacillus migulanus. Management and Innovation Technology International Conference (MITicon), pp. MIT-209. CR - Comăniță ED, Hlihor RM, Ghinea C, Gavrilescu M. (2016). Occurrence of plastic waste in the environment: ecological and health risks. Environ. Eng. Manag. J., 15: 675–685. CR - Dang TCH., Nguyen DT, Thai H, Nguyen TC, Tran TTH., Le VH, Nguyen QT (2018). Plastic degradation by thermophilic Bacillus sp. BCBT21 isolated from composting agricultural residual in Vietnam. Advances in Natural Sciences: Nanoscience and Nanotechnology, 9(1), 015014. CR - Danso D., Schmeisser C., Chow J, Zimmermann W, Wei R, Leggewie C, Streit WR (2018). New insights into the function and global distribution of polyethylene terephthalate (PET)-degrading bacteria and enzymes in marine and terrestrial metagenomes. Appl. Environ. Microbiol., 84(8): e02773-17. CR - Demirkan E, Sevgi T, Guler BE, Zeren B, Ozalpar B, Abdou M (2019). Investigation of potential use in bioremediation of Bacillus sp. strains isolated from Turkish soils. Karaelmas Science and Engineering Journal, 9(1): 107-113. CR - Dussud, C, Ghiglione, JF (2014). Bacterial degradation of synthetic plastics. In CIESM Workshop Monogr., 46: 49-54. CR - Esmaeili A, Pourbabaee AA, Alikhani HA, Shabani F, Esmaeili E (2013). Biodegradation of Low-Density Polyethylene (LDPE) by Mixed Culture of Lysinibacillus xylanilyticus and Aspergillus niger in Soil. PLOS. ONE, 8. CR - Garside M, (2019). Global plastic production from 1950 to 2017. Statista, online at https://www.statista.com/statistics/282732/global-production-of-plastics-since-1950/ CR - Geyer R, Jambeck JR, Law KL (2017). Production, use, and fate of all plastics ever made. Science advances, 3(7): e1700782. CR - Ghosh SK, Pal S, Ray S (2013). Study of microbes having potentiality for biodegradation of plastics. Environmental Science and Pollution Research, 20(7): 4339-4355. CR - Gong J, Kong T, Li Y, Li Q, Li Z, Zhang J (2018). Biodegradation of microplastic derived from poly (ethylene terephthalate) with bacterial whole-cell biocatalysts. Polymers, 10(12): 1326. CR - Huang Y, Xiao L, Li F, Xiao M, Lin D, Long X, Wu Z (2018). Microbial degradation of pesticide residues and an emphasis on the degradation of cypermethrin and 3-phenoxy benzoic acid: a review. Molecules, 23(9): 2313. CR - Keay L, Wildi BS (1970). Proteinases of the genus Bacillus. I. Neutral proteinases. Biotechnol. Bioeng., 12: 179-212. CR - Koitabashi M, Sameshima-Yamashita Y, Watanabe T, Shinozaki Y, Kitamoto H (2016). Phylloplane fungal enzyme accelerate decomposition of biodegradable plastic film in agricultural settings. Japan Agricultural Research Quarterly, 50: 329-324. CR - Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR (2012). Biodegradation of low density polythene (LDPE) by Pseudomonas species. Indian Journal of Microbiology, 52: 411-419. CR - Mahdi MS, Ameen RS, Ibrahim HK (2016). Study on degradation of nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241). Int J Adv Res Biol Sci., 3: 200-209. CR - Muhonja CN, Makonde H, Magoma G, Imbuga M. (2018). Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi-Kenya. PloS one, 13(7): e0198446. CR - Neufeld L, Stassen F, Sheppard R, Gilman T (2016). The new plastics economy: rethinking the future of plastics. In World Economic Forum. CR - Odusanya SA, Nkwogu JV, Alu N, Udo GE, Ajao JA, Osinkolu GA, Uzomah AC (2013). Preliminary studies on microbial degradation of plastics used in packaging potable water in Nigeria. Nigerian Food Journal, 31(2): 63-72. CR - Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4): 406-425. CR - Shah AA, Hasan F, Hameed A, Ahmed S (2008). Biological degradation of plastics: A comprehensive review. Biotechnology Advances, 26: 246-265. CR - Shimao M (2001). Biodegradation of plastics. Curr. Opinion Biotechnol., 12: 242-247. CR - Sudhakar M, Priyadarshini C, Doble M, Murthy PS, Venkatesan R (2007). Marine bacteria mediated degradation of nylon 66 and 6. International Biodeterioration & Biodegradation, 60(3): 144-151. CR - Sugihara A, Tani T, Tominaga Y (1991). Purification and characterization of a novel thermostable lipase from Bacillus sp. The Journal of Biochemistry, 109(2): 211-216. CR - Thompson JD, Higgins DG, Gibson TJ (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-spesific gap penalties and weight matrix choice. Nuc. Acids Res., 22: 4674-4680. CR - Tokiwa Y, Calabia BP, Ugwu CU, Aiba S (2009). Biodegradability of plastics. International Journal of Molecular Sciences, 10(9): 3722-3742. CR - Trevino AL, Garcia G (2011). Polyurethane foam as substrate for fungal strains. Advances in Bioscience and Biotechnology, 2: 52-58. CR - Urbanek AK, Rymowicz W, Mirończuk AM (2018). Degradation of plastics and plastic-degrading bacteria in cold marine habitats. Applied Microbiology and Biotechnology, 102(18): 7669-7678. CR - Usha R, Sangeetha T, Palaniswamy M (2011). Screening of Polyethylene degrading microorganisms from garbage soil. Libyan Agricultural Research Center Journal International, 2: 200-204. CR - Webb H (2012). Biodegradation of poly(ethylene terephthalate) by marine bacteria, and strategies for its enhancement, Doctor of Philosophy, Swinburne University of Technology, Faculty of Life and Social Sciences, Environmental and Biotechnology Centre. CR - Yoo YJ, Hong J, Hatch RT (1987). Comparison of a-amylase activities from different assay methods. Biotechnol. Bioeng., 30:147-51. CR - Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, et al. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278):1196-1199. UR - https://dergipark.org.tr/en/pub/jbes/issue//1680081 L1 - https://dergipark.org.tr/en/download/article-file/4790246 ER -