TY - JOUR T1 - Effects of substrate formulation on yield and yield components in enoki (Flammulina velutipes) cultivation AU - Çömlekçioğlu, Nuray AU - Tuğa, Süheyla AU - Soylu, Mustafa PY - 2025 DA - June Y2 - 2025 JF - International Journal of Agriculture Forestry and Life Sciences JO - Int J Agric For Life Sci PB - Volkan OKATAN WT - DergiPark SN - 2602-4381 SP - 26 EP - 33 VL - 9 IS - 1 LA - en AB - Mushroom cultivation is considered a technology that transforms waste into valuable food and it is an important technology for sustainable agriculture and waste management. This study was conducted to evaluate the cultivation potential of Flammulina velutipes (enoki) mushroom on different agricultural waste-based substrates. Nine different substrate formulations were prepared using locally available wastes such as cotton gin waste, poplar sawdust, wheat bran, and compost. Barley spawn of enoki (EN141-Chinese-Korean hybrid) was cultivated. The physicochemical properties of the substrates (moisture, pH, organic matter, C/N ratio) and fungal growth parameters (mycelial growth duration, primordium formation, yield, biological efficiency-BE, protein and mineral content) were investigated. According to the results, the substrates containing 100% cotton gin waste and 80% cotton gin waste + 20% wheat bran provided the highest yield (197.05 g/kg and 185.74 g/kg, respectively) and BE (69.27% and 58.20%, respectively). However, protein content was relatively low in these substrates (20.95-27.58%, respectively). In contrast, the substrate containing 80% sawdust+15% wheat bran+ 5% carboxymethyl cellulose offered the highest protein content (52.85%), but was disadvantageous in terms of yield and fruiting body weight. Compost-based substrates did not support mycelial growth. Mushrooms grown on sawdust-based substrates had high levels of phosphorus, potassium and microelements. The study revealed that substrate selection significantly influenced yield, quality and production time in enoki cultivation. Local sources such as cotton gin waste were found to be suitable for high yields, but substrate optimization was needed to increase protein and mineral content. These findings have important implications for sustainable mushroom cultivation and utilization of agricultural wastes. KW - Substrate optimization KW - enoki KW - biological efficiency KW - agricultural waste KW - protein CR - Açıkgöz, N., İlker, E., Gökçöl, A. (2004). Evaluation of biological research on computer, Ege University Seed Technology Application and Research Center Publications No:2, Ege University Faculty of Agriculture Offset Workshop, Bornova-İzmir, 202 p. CR - Akindahunsi, A. A., Oyetayo, F. L. (2006). Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (fries) singer. LWT, 39, 548–553. CR - Azeem, U., Hakeem, K. R., Ali, M. (2020). Fungi for human health. Current Knowledge and Future Perspectives. Springer Cham., pp. 5–11. doi: 10.1007/978-3-030-58756-7. CR - Bains, A., Chawla, P., Kaur, S., Najda, A., Fogarasi, M., Fogarasi, S. (2021). Bioactives from mushroom: health attributes and food industry applications. Materials, 14(24), 7640. doi: 10.3390/ma1424764. CR - Barmon, B.K., Imrana, S., Parvez, K.A. Mamun, A. (2012). Economics of Mushroom (Agaricus bisporus) Production in a Selected Upazila of Bangladesh, Department of Economics, East West University, Dhaka, Bangladesh. A Scientific Journal of Krishi Foundation Index Journal The Agriculturists. 10 (2). 77-89. CR - Baybas,B. 2023. Determination of usage possibilities of olive pomace as substrate material in the cultivation of enoki mushroom (Flammulina velutipes) in bottle culture. Bolu Abant İzzet Baysal University, Institute of Science, Master's Thesis, Bolu, (In Turkish) CR - Bernabé-González, T., Cayetano-Catarino, M., Bernabé-Villanueva, G., Romero-Flores, A., Ángel-Ríos, MD, Pérez-Salgado, J. (2015) Meksika'daki Tarımsal Yan Ürünlerde Ganoderma lucidum'un Yetiştirilmesi. Micologia Aplicada Uluslararası 27(2):25-30. CR - Cormican, T. Staunton, L. (1991). Factors in mushroom (Agaricus bisporus) compost productivity. Mushroom Science. 13. 221-226. CR - Dowom, S. A., Rezaeian, S., Pourianfar, H. R. 2019. Agronomic and environmental factors affecting cultivation of the winter mushroom or Enokitake: achievements and prospects. Applied Microbiology and Biotechnology, 103:2469–2481. https://doi.org/10.1007/s00253-019-09652-y. CR - Fidler G, Rodino S, Butu A, Butu M, Popa G, Cornea CP (2015) Optimization of submerged culture conditions for Flammulina velutipes on SBD culture medium. J Biotechnol 208:S103. https://doi.org/10.1016/j.jbiotec.2015.06.324. CR - Ge ZW, Liu XB, Zhao K, Yang ZL (2015) Species diversity of Flammulina in China: new varieties and a new record. Mycosystema 34:589–603. https://doi.org/10.13346/j.mycosystema.150080 CR - Gupta, S., Summuna, B., Gupta, M., Annepu, S. K. (2018). Edible mushrooms: cultivation, bioactive molecules, and health benefits. Bioactive Molecules in Food, 1, 1–33. CR - Han J, Sun R, Huang C, Xie H, Gao X, Yao Q, Yang P, Li J, Gong Z. Effects of Different Carbon and Nitrogen Ratios on Yield, Nutritional Value, and Amino Acid Contents of Flammulina velutipes. Life (Basel). 2024 May 8;14(5):598. https://doi:10.3390/life14050598. PMID: 38792619; PMCID: PMC11122278. CR - Harith N, Abdullah N, Sabaratnam V (2014) Cultivation of Flammulina velutipes mushroom using various agro-residues as a fruiting substrate. Pesqui Agropecu Bras 49:181–188. https://doi.org/10.1590/S0100-204X2014000300004. CR - Hassan FRH, Ghada MM, El-Kady AT (2012) Mycelial biomass production of enoke mushroom (Flammulina velutipes) by submerged culture. Aust J Basic Appl Sci 6:603–610. CR - Hiramori, C., Koh, K., Kurata, S., Ueno, Y., Gamage, S., Huang, P. & Ohga, S. (2017). Cultivation of Flammulina velutipes on modified substrate using fermented apple pomace. Advances in Microbiology. 7 (11). 719-728. https://doi.org/10.1051/e3sconf/20244820101. CR - Hughes KW, McGhee LL, Methven AS, Johnson JE, Petersen RH (1999). Patterns of geographic speciation in the genus Flammulina based on sequences of the ribosomal ITS1-5.8 S-ITS2 area. Mycologia 1:978–986. https://doi.org/10.2307/3761628 CR - Imtiaj, A., Rahman, S.A. (2008). Economic viability of mushrooms cultivation to poverty reduction in Bangladesh. Tropical and Subtropical Agroecosystems. 8. 93-99. CR - Ji, H., Wang, Q., Wang, H., Chen, W. J., Zhu, Z. H., Hou, H., Zhang, W. (2001). Preliminary research on Flammulina velutipes and Ganoderma lucidum cultivation using maize straw. . Edible Fungi China, 20(6), 11-12. CR - Jung, K. J., Choi, D. S., Bang, G. P., Chung, K. C. (2009). Optimum mixing rate of used media for saving the production cost of Flammulina velutipes. Journal of Mushroom, 7(1), 22-26. CR - Kacar, B., İnal, A. (2008). Plant analysis. Nobel Publishing Distribution. Publication No:1241. ISNB 978-605-395-036-3(In Turkish). CR - Karasoy, A. F. (2022). Effect of growth media prepared with different additives on the yield and quality of Flammulina velutipes mushroom. Ondokuz Mayıs University, Institute of Science, Master Thesis, Samsun, (In Turkish). CR - Khan F, Chandra R (2017) Effect of physiochemical factors on fruiting body formation in mushroom. Int J Pharm Pharm Sci 9:33–36. https://doi.org/10.22159/ijpps.2017v9i10.20086. CR - Khereba, A.H, Farrag, A.M., Hassan, F. R.H., Waheed, M. (2010). Influence of some agrıcultural wastes on growth, yıeld and nutrıtıonal values of wınter mushroom (Flammulina velutipes (Curtis) Singer). Egyptian Journal of Agricultural Sciences. 61. 299-306. https://doi.org https://doi.org/10.21608/ejarc.2010.215471. CR - Kozhemyakina N.V., Ananyeva E.P, Gurina S.V., Galynkin, V.A. (2010) Conditions of cultivation, composition, and biological activity ofmycelium of Flammulina velutipes (Fr.) P. Karst. Appl. Biochem. Microbiology. l46:536–539. https://doi.org/10.1134/S0003683810050121. CR - Kurata, S. & Koh, K. 2017. Potential of fermented sweet corn stover as a substitute for corncob in mushroom (Flammulina velutipes ) substrate. Journal of Advanced Agricultural Technologies, 4(2), 165-169. CR - Leifa, F., Pandey, A., Soccol, C. R. (2001). Production of Flammulina velutipes on coffee husk and coffee spent-ground. Brazilian Archives of Biology and Technology, 44, 205-212. CR - Liao, Q., Zhao, Z., Cui, R., Gong, M., Xu, C., Tu, S. (2019). Effect of rape straw on the growth of Flammulina velutipes . AIP Conference Proceedings, 2079, 020023. CR - Listiana, I., Fahda, N.M. Satitiningrum, Y. Oktafiani, R., Kesumawardani, A.D. (2024). Identification of Macroscopic Fungi in the Gedong Wani Production Forest Area, South Lamping. In E3S Web of Conferences (Vol. 482, p. 01011). EDP Sciences. https://doi.org/10.1051/e3sconf/202448201011 CR - Liu, X. B., Li, J. Yang, Z. L. (2018). Genetic diversity and structure of core collection of winter mushroom (Flammulina velutipes) developed by genomic SSR markers. Hereditas, 155, 1-8. https://doi.org/10.1186/s41065-017-0038-0. CR - Manzi, P., Gambelli, L., Marconi, S., Vivanti, V., Pizzoferrato, L. (1999). Nutrients in edible mushrooms: an inter-species comparative study. Food Chemistry, 65, 477–482. CR - Miao, R., Zhou, J., Tan, W., Peng, W., Gan, B., Tang, L. Huang, Z. (2014). A preliminary screening of alternative substrate for cultivation of Flammulina velutipes. Mycosystema, 33(2), 411-424. CR - Okuyucu, H. (2021). Effects of different growing media on the yield and quality of Flammulina velutipes mushroom. Ondokuz Mayıs University, Institute of Science, Master Thesis, Samsun (In Turkish) CR - Osman M, Ahmed W, Hussein F, El-Sayed H (2014) Endopolysaccharides production and growth of Flammulina velutipes 6 under submerged conditions. Chem Biol Phys Sci 4:3350–3360. CR - Rezaeian S, Pourianfar HR (2017) A comparative study on bioconversion of different agro wastes by wild and cultivated strains of Flammulina velutipes. Waste Biomass Valorization 8:2631–2642. https://doi.org/10.1007/s12649-016-9698-7. CR - Royse, D. J. (1985). Effects of spawn run time and substrate nutrition on yield and size of the shiitake mushroom. Mycologia, 77(5), 756-762. CR - Royse, D. J., Baars, J., Tan, Q. (2017). Current Overview of Mushroom Production in the World. In: Diego, C. Z., Pardo-Giménez,A. (eds.). Edible and Medicinal Mushrooms: Technology and Applications. Chichester, West Sussex: John Wiley & Sons Ltd. pp. 5–13. doi: 10.1002/9781119149446.ch2. CR - Sangkaew, M. Koh, K. (2017). The cultivation of Flammulina velutipes by using sunflower residues as mushroom substrate. Journal of Advanced Agricultural Technologies, 4(2), 140-144. CR - Song, C. H., Lee, C. H., Huh, T. L., Ahn, J. H. Yang, H. C. (1993). Development of substrates for the production of basidiocarps of Flammulina velutipes . The Korean Journal of Mycology, 21(3), 212-216. CR - Tang, X. N., Bian, G. Q., Zhang, M. (2001). Studies on cultivating Flammulina velutipes with Paspalum notatum. Edible Fungi of China, 20(4), 10-11. CR - Xie, C., Gong, W., Yan, L., Zhu, Z., Hu, Z., Peng, Y. (2017). Biodegradation of ramie stalk by Flammulina velutipes: Mushroom production and substrate utilization. Amb Express, 7, 171. UR - https://dergipark.org.tr/en/pub/ijafls/issue//1681035 L1 - https://dergipark.org.tr/en/download/article-file/4794368 ER -