TY  - JOUR
T1  - Fusarium proliferatum: isolation, identification and evaluation of its association with the model insect Galleria mellonella (L.) (Lepidoptera: Pyralidae)
TT  - Fusarium proliferatum: izolasyonu, tanımlaması ve model Böcek Galleria mellonella (L.) (Lepidoptera: Pyralidae) ile ilişkisinin değerlendirilmesi
AU  - Güner, Pınar
AU  - Er, Aylin
AU  - Aşkun, Tülin
AU  - Deniz Sönmez, Görkem
AU  - Şengül, Selin Meryem
PY  - 2025
DA  - July
Y2  - 2025
DO  - 10.35229/jaes.1681652
JF  - Journal of Anatolian Environmental and Animal Sciences
JO  - JAES
PB  - Bülent VEREP
WT  - DergiPark
SN  - 2548-0006
SP  - 416
EP  - 423
VL  - 10
IS  - 4
LA  - en
AB  - Although the genus Fusarium is widely recognized for including significant plant pathogens and mycotoxin producers, many Fusarium species have also demonstrated promising potential for insect control. These species exhibit characteristics such as high mortality rates, rapid action, and prolific sporulation, making them attractive candidates for agricultural pest management. In this study, the micro and macromorphology, DNA sequencing, and the effects on the model insect Galleria mellonella of Fusarium proliferatum isolated from soil using the trap method were investigated. Morphological analyses revealed detailed colony and spore structures on various agar media. Species identification was confirmed through DNA analysis using ITS and CaM gene sequences. F. proliferatum was observed to prolong the larval stage of model ınsect G. mellonella while reducing pupal duration, weight, and total egg production. Our findings indicate that the fungus exerts a dose-dependent impact on the development and reproduction of G. mellonella. Future studies should focus on identifying secondary metabolites responsible for the observed biological effects, evaluating possible effects on non-target organisms, and conducting field trials under different environmental conditions.
KW  - Fusarium proliferatum
KW  - Galleria mellonella
KW  - developmental biology
KW  - fungal pathogenicity
KW  - identification
KW  - isolation
N2  - Fusarium cinsi, önemli bitki patojenleri ve mikotoksin üreticileri içermesiyle dikkat çekmesine rağmen birçok Fusarium türünün böcekleri kontrol etmede etkili olduğu ve yüksek ölüm oranlarına neden olma, hızlı etki ve bol sporlanma gibi tarımsal zararlı kontrolü için umut verici özellikler sergilediği bilinmektedir. Bu çalışmada, topraktan tuzak metodu ile izole edilen Fusarium proliferatum'un mikro-makro morfolojisi, DNA dizilimi ve model böcek Galleria mellonella üzerindeki etkisi araştırılmıştır. Morfolojik analizler, farklı agarlarda ayrıntılı koloni ve spor yapıları ortaya koymuştur. DNA analizi ile ITS ve CaM gen dizileri kullanılarak tür tanımlamasını doğrulanmıştır. F. proliferatum'un model böcek G. mellonella 'nın larval evresi uzattığı ve pupa süresini, ağırlığını ve toplam yumurta sayısını azalttığı gözlenmiştir. Çalışmamız fungusun G. mellonella 'nın gelişme ve üremesi üzerinde doza bağlı bir etkiye sahip olduğunu göstermektedir. Gelecekteki çalışmalar, gözlenen biyolojik etkilerden sorumlu ikincil metabolitlerin tanımlanmasına, hedef dışı organizmalar üzerindeki olası etkilerin değerlendirilmesi ve farklı çevre koşulları altında saha denemelerinin yürütülmesine odaklanmalıdır.
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UR  - https://doi.org/10.35229/jaes.1681652
L1  - https://dergipark.org.tr/en/download/article-file/4797091
ER  -