@article{article_1684694, title={Ameliorative Effects of Alpha-tocopherol and Crithmum Martimum L. Against Hydrogen Peroxide-induced Toxicity on Dental Pulp Stem Cells In Vitro}, journal={Meandros Medical And Dental Journal}, volume={26}, pages={363–373}, year={2025}, DOI={10.69601/meandrosmdj.1684694}, author={Mergen Dalyanoğlu, Mukaddes and Seçme, Mücahit and Eroğlu Güneş, Canan and Küçükatay, Vural and Dodurga, Yavuz and Çelik, Ali and Turgut, Sebahat}, keywords={alpha-tocopherol, Crithmum martimum L. essential oil, toxicity, Dental Pulp Stem Cell, hydrogen peroxide}, abstract={Objective: Tooth-bleaching agents contain peroxide and are asserted to cause cellular damage. The study aims to evaluate the effects of the application of hydrogen peroxide (HP) on dental pulp stem cells (DPSCs) in vitro and whether alpha-tocopherol (AT) and Crithmum maritimum L. (CM) have protective effects against potential damage to occur. Materials and Methods: HP, AT and CM doses applied in the experiment were determined according to the xtt analysis results. DPSCs were treated with 2 or 6 μg/mL HP, also 100 µM AT or 2 µg/mL CM followed by two doses of HP, DNA damage levels were measured using comet assay in 0, 24, 72 hours.
Results: Tail length, as a indicative of dna damage increased significantly in groups that applied only HP, compared to the control group. In contrast, the tail length of the groups that applied AT and CM was significantly shorter than that only HP-treated groups. The tail intensity and tail moment averages of all groups increased significantly than the control group at all times, except the mean tail moment of CM6HP, AT2HP groups, and mean tail intensity of the CM6HP group on the 72 hour. The tail intensity and tail moment averages of 6HP groups exhibited a significant increase compared to CM and AT applied groups at all times, except the AT6HP group on the 24th hour. The averages of 2HP group demonstrated a significant increase on the 24th hour compared to CM and AT applied groups, while no significance was observed on 0 and 72nd hours. Conclusion: HP causes genotoxicity. AT and CM, however, reduce DNA damage, significantly.}, number={3}, publisher={Aydin Adnan Menderes University}, organization={This study was supported by grant 2014SBE005 from Pamukkale University Scientific Research Projects Coordination Unit (PAÜBAP)}