TY - JOUR T1 - Gel of Monascus purpureus JmbA rice accelerate wound healing in diabetic rats AU - Sutejo, Ika AU - Wisudanti, Desie Dwi AU - Nisa, Alfira Khoirun AU - Goestien, Alya Febriana AU - Widiastuti, Latiefah Noer AU - Nurhidayat, Novik AU - Sugiyanta, Sugiyanta AU - Santosa, Ali PY - 2025 DA - June JF - Journal of Research in Pharmacy JO - J. Res. Pharm. PB - Marmara University WT - DergiPark SN - 2630-6344 SP - 699 EP - 707 VL - 28 IS - 3 LA - en AB - The most frequent complication of diabetes mellitus (DM) is diabetic ulcer, with a prevalence of 15-20% and an amputation rate of 30%. There is no effective and easy-to-apply treatment for acute-phase open wounds of patients with DM to prevent their development into ulcers. Monascus purpureus jmbA Rice (MJR) from Jember is superior to similar products from other regions in Indonesia because it has the highest monacolin content with an antioxidant effect. The study aimed to prove the effectiveness of MJR gel in accelerating the healing of acute wounds of diabetic rats. This true experimental laboratory research was a posttest-only control group design. We used Rattus norvegicus Wistar rats, 3-4 months old, weighing 200-300 grams. The 36 rats were divided into four treatment groups. The control group was given a gel of MJR 0%. The positive control group was given the antibiotic and placenta extract gel combination, and treatment groups T1 and T2 were given gel MJR40% and MJR80%. Excision wounds were made as deep as the subcutaneous layer on the back of the hyperglycemic rat. Four rats were terminated from each group on days 4, 11, and 18, and skin tissue was taken to observe the percentage of wound area shrinkage, epithelial thickness, and fibroblast count and analyze the levels of hydroxyproline and MDA. The MJR80% group had the highest percentage of wound area shrinkage, epithelial thickness, and fibroblast count compared to other groups. MDA levels decreased with increasing days in all treatment groups. Hydroxyproline levels increased on days 4 and 11, then decreased on day 18 in all treatment groups. We conclude that the 80% MJR group revealed the best acute diabetic wound healing. It was not statistically different from the combination of antibiotic and placenta extract gel treatment group. KW - Diabetes Mellitus KW - Fibroblast KW - Hydroxyproline KW - MDA KW - Monascus purpureus KW - Wound Healing CR - [1] Patel S, Srivastava S, Singh MR, Singh D. Mechanistic ınsight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing. Biomed Pharmacother. 2019; 112:108615. https://doi.org/10.1016/j.biopha.2019.108615 CR - [2] Burgess JL, Wyant WA, Abujamra BA, Kirsner RS, Jozic I. Diabetic wound-healing science. Medicina. 2021;57(10): 1072. https://doi.org/10.3390/medicina57101072 CR - [3] Shi YC, Pan TM. Anti-diabetic effects of Monascus purpureus NTU 568 fermented products on streptozotocin-ınduced diabetic rats. J Agric Food Chem. 2010;58(13):7634–7640. https://doi.org/10.1021/jf101194f CR - [4] Lu KY, Chen SH, Lin YS, Wu HP, Chao PM. An Antidiabetic nutraceutical combination of red yeast rice (Monascus purpureus), bitter gourd (Momordica charantia), and chromium alleviates dedifferentiation of pancreatic β cells in db/db mice. Food Sci Nutr. 2020;8(12):6718–6726. https://doi.org/10.1002/fsn3.1966 CR - [5] Lai JR, Hsu YW, Pan TM, Lee CL. Monascin and ankaflavin of Monascus purpureus prevent alcoholic liver disease through regulating AMPK-mediated lipid metabolism and enhancing both anti-ınflammatory and anti-oxidative systems. Molecules. 2021;26(20): 6301. https://doi.org/10.3390/molecules26206301 CR - [6] Lin CS, Hur HF, Lin CC. Antioxidant properties and antibacterial activity of fermented Monascus purpureus extracts 50 determination of total phenolics. MOJ Food Proc Technol. 2019;7(2): 49-54. https://doi.org/10.15406/mojfpt.2019.07.00219 CR - [7] Gutierrez GE, Mundy B, Rossini G, Garrett IR, Chen ST, Mundy GR. Red yeast rice stimulates bone formation in rats. Nutr Res. 2006; 26(3): 124-129. https://doi.org/10.1016/j.nutres.2006.02.006 CR - [8] Kasim E, Kurniawati Y, Nurhidayat N. Use of local isolate of Monascus purpureus for reducing blood cholesterol in Sprague Dawley rat. Biodiversitas J Biol Divers. 2006;7(2):123-126. https://doi.org/10.13057/biodiv/d070206 CR - [9] Kasim E, Triana E, Yulinery T, Nurhidayat N. Pengaruh Angkak Hasil Fermentasi Beras oleh Monascus purpureus JMBa terhadap Aktivitas Antioksidan dan Glutathion Peroksidase (GPx) serta Histopatologi Hati Tikus Galur Sprague dawley. Berita Biologi. 2012;11(2):177-184. https://doi.org/10.14203/beritabiologi.v11i2.487 CR - [10] Wijaya H, Novitasari, Jubaidah S. Perbandingan Metode Ekstraksi terhadap Rendemen Ekstrak Daun Rambui Laut (Sonneratia caseolaris L. engl). Jurnal Ilmiah Manuntung. 2018;4(1):79-83. https://doi.org/10.51352/jim.v4i1.148 CR - [11] Simons M, Rubanyi GM. Modern Concepts in Angiogenesis. London: Imperial College Press. 2007. https://doi.org/10.1142/p491 CR - [12] Primadina N, Basori A, Perdanakusuma DS. Proses penyembuhan luka ditinjau dari aspek mekanisme seluler dan molekuler. Qanun Medika. 2019;3(1):31–43. https://doi.org/10.30651/jqm.v3i1.2198 CR - [13] Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconst Surg. 2016; 117(7s): 12-34. https://doi.org/10.1097/01.prs.0000225430.42531.c2 CR - [14] Kumar V, Buku Ajar Patologi Dasar, Edisi kesepuluh, Elsevier, Jakarta, 2018. CR - [15] Craig W, Chen J, Richardson D, Thorpe R, Yuan Y. A highly stereoselective and scalable synthesis of l -allo-enduracididine. Organic Lett. 2015;17(18):4620–4623. https://doi.org/10.1021/acs.orglett.5b02362 CR - [16] Werdhasari A. Peran Antioksidan bagi Kesehatan. Jurnal Biomedik Medisiana Indonesia. 2014;3(2):59–68. https://doi.org/10.22435/jbmi.v3i2.1659 CR - [17] Pandiangan JFE, Putra INK, Pratiwi IDPK. Utilization of angkak as natural colorant and antioxidant on mackerel fish sausage (Rastrelliger kanagurta L.). J Food Sci Technol. 2019;8(2):197-206. https://doi.org/10.24843/itepa.2019.v08.i02.p10 CR - [18] Magnani F, Mattevi A. Structure and Mechanisms of ROS Generation by NADPH Oxidases. Curr Opin Struct Biol. 2019; 59: 91–97. https://doi.org/10.1016/j.sbi.2019.03.001 CR - [19] Elfiah U, Naufal MF, Hidayat MA. Effect of Robusta coffee extract gel on fibroblast and collagen during proliferative phase of IIB degree-burn on Long Evans rats. J Med Sci (Berkala Ilmu Kedokteran). 2022; 54(3): 202-210. https://doi.org/10.19106/JMedSci005403202201 CR - [20] Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofibroblasts and mechano: Regulation of connective tissue remodelling. Vol. 3. Nature Rev Mol Cell Biol. 2002; 3: 349–363. https://doi.org/10.1038/nrm809 CR - [21] Castro B, Palomares T, Azcoitia I, Bastida F, del Olmo M, Soldevilla JJ, Alonso-Varona A. Development and preclinical evaluation of a new galactomannan-based dressing with antioxidant properties for wound healing. Histol Histopathol. 2015; 30(12): 1499–1512. https://doi.org/10.14670/hh-11-646 CR - [22] Cialdai F, Risaliti C, Monici M. Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Frontiers Bioeng Biotechnol. 2022; 10:958381. https://doi.org/10.3389/fbioe.2022.958381 CR - [23] Ramirez H, Patel SB, Pastar I. The role of TGFβ signaling in wound epithelialization. Adv Wound Care (New Rochelle). 2014; 3(7):482–491. https://doi.org/10.1089%2Fwound.2013.0466 CR - [24] Zulkefli N, Che Zahari CNM, Sayuti NH, Kamarudin AA, Saad N, Hamezah HS, Bunawan H, Baharum SN, Mediani A, Ahmed QU, Ismail AFH, Sarian MN. Flavonoids as potential wound-healing molecules: Emphasis on pathways perspective. Int J Mol Sci. 2023; 24(5):4607. https://doi.org/10.3390/ijms24054607 CR - [25] Aulia L, Pane YS. Effect of Aloe vera extract in post-burn skin repair in rats. F1000Res. 2022; 11:168. https://doi.org/10.12688/f1000research.79538.2 CR - [26] Sujono TA, Hidayah UNW, Sulaiman TNS. Efek Gel Ekstrak Herba Pegagan (Centella asiatica L. urban) dengan Gelling Agent Hidroksipropil Methylcellulose terhadap Penyembuhan Luka Bakar pada Kulit Punggung Kelinci. Biomedika. 2014;6(2): 9-17. https://doi.org/10.23917/biomedika.v6i2.276. CR - [27] Husna F, Suyatna FD, Arozal W, Purwaningsih EH. Model Hewan Coba pada Penelitian Diabetes. Pharm Sci Res. 2019;6(3):131–141. http://dx.doi.org/10.7454/psr.v6i3.4531 CR - [28] Marchianti ACN, Sakinah EN, Elfiah U, Putri NKS, Wahyuliswari DI, Maulana M, Ulfa EU. Gel formulations of Merremia mammosa (lour.) accelerated wound healing of the wound in diabetic rats. J Tradit Complement Med. 2021;11(1):38–45. https://doi.org/10.1016/j.jtcme.2019.12.002 CR - [29] Glozer K. Protocol for Leaf Image Analysis – Surface area [Internet]. 2008. Available from: http://rsb.info.nih.gov/ij/download.html CR - [30] Gupta R, Garg A, Sharma P, Pandey P. Wound healing and antioxidant effect of Calliandra haematocephala leaves on ıncision and excision wound models. Asian J Pharm Pharmacol. 2016;2(2): 34-39. CR - [31] Aprini UR, Novianry V, Zakiah M. Pengaruh pemberian astaxanthin terhadap kadar malondialdehid pada kerusakan jaringan testis tikus putih yang diinduksi formaldehid secara oral. Jurnal Mahasiswa PSPD FK Universitas Tanjungpura. 2019;5(1):1234–1247. CR - [32] Sutejo I. Effectiveness of edamame (Glycine max L. Merril) membrane in accelerating the wound healing process of deep-partial thickness burn. Hacettepe Univ J Fac Pharm. 2023; 43(2): 120-127. https://doi.org/10.52794/hujpharm.1111499 UR - https://dergipark.org.tr/en/pub/jrespharm/issue//1686091 L1 - https://dergipark.org.tr/en/download/article-file/4815781 ER -