TY  - JOUR
T1  - Microscopic Evaluation and Qualitative Phytochemical Screening of Leaves and Fruits of Lycium ferocissimum Miers.
AU  - Atlı, Beste
AU  - Ghahramani, Sayeh
AU  - Ak-sakallı, Ezgi
AU  - Öztinen, Nesrin
AU  - Koşar, Müberra
PY  - 2025
DA  - July
Y2  - 2025
DO  - 10.54994/emujpharmsci.1686372
JF  - EMU Journal of Pharmaceutical Sciences
JO  - EMUJPharmSci
PB  - Dogu Akdeniz University
WT  - DergiPark
SN  - 2651-3587
SP  - 20
EP  - 29
VL  - 8
IS  - 1
LA  - en
AB  - Lycium ferocissimum Miers. is a plant species belonging to the Solanaceae family. This study aimed to investigate the microscopic structure and phytochemical composition of the leaf, green fruit, and red fruit of L. ferrocissimum. Plant materials were oven-dried at 50 °C, powdered, and prepared for microscopic analysis using chloral hydrate. Microscopy revealed stomata, epidermis, crystals, and secretory hairs in leaves; and parenchyma, pigment cells, and stone cells in fruits. Phytochemical screening was conducted using standard biochemical reactions for primary and secondary metabolites. Alkaloids were confirmed by Dragendorff’s test, producing a reddish-brown precipitate. Carbohydrates were detected using Fehling, Molisch, and Seliwanoff tests, all giving positive results. Flavonoid glycosides were identified via Cyanidin test; red fruit gave a strong orange color and zinc confirmed the presence of flavonoid. Lipid detection using Sudan III resulted in orange spots in both red and green fruits. Carotenoids were identified by pH-dependent color changes. No saponins were detected.
KW  - Lycium ferocissimum
KW  - phytochemical analysis
KW  - microscopy
KW  - flavonoids
KW  - medicinal plant
CR  - Chen H, Olatunji OJ, Zhou Y (2016). Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice. J Nat Med 70: 610–619.
CR  - Dhar P, Tayade A, Ballabh B, Chaurasia OP, Bhatt RP, et al. (2011). Lycium ruthenicum Murray: a less-explored but high-value medicinal plant from Trans-Himalayan cold deserts of Ladakh, India. Plant Archives 11(2): 583-586.
CR  - Jiang Y, Fang Z, Leonard W, Zhang P (2021). Phenolic compounds in Lycium berry: Composition, health benefits and industrial applications. J Funct Foods 77: 104340.
CR  - Joubert AM, Verhoeven RL, Venter HJT (1984). An anatomical investigation of the stem and leaf of the South African species of Lycium L. (Solanaceae). South African Journal of Botany 3(4): 219-230.
CR  - Konarska A (2018). Microstructural and histochemical characteristics of Lycium barbarum L. fruits used in folk herbal medicine and as functional food. Protoplasma 255(6): 1839-1854.
CR  - Kosar M, Karatoprak GS, Atlı B, Ilgün S, Kongul Safak E, et al. (2024). Unveiling the Antioxidant, Cytotoxic, and Anti-Inflammatory Activities and Chemical Compositional Information of an Invasive Plant: Lycium ferocissimum Miers. Plants 13(7): 1035.
CR  - Kumar R, Sharma R, Thakur MS, Saxena S, Kaur A (2022). Comparative study of phytochemicals, antioxidant activities and chromatographic profiling of different parts of Lycium ruthenicum Murr of Trans-Himalayan region. Phytomedicine Plus 2(4): 100339.
CR  - Lev E, Amar Z (2006). Reconstruction of the inventory of materia medica used by members of the Jewish community of medieval Cairo according to prescriptions found in the Taylor-Schechter Genizah collection, Cambridge. J Ethnopharmacol 108: 428–444.
CR  - Liu SC, Lin JT, Hu CC, Shen BY, Chen TY et al. (2017) Phenolic compositions and antioxidant attributes of leaves and stems from three inbred varieties of Lycium chinense Miller harvested at various times. Food Chemistry 215: 284-291.
CR  - Qian D, Zhao Y, Yang G, Huang L (2017). Systematic review of chemical constituents in the genus Lycium (Solanaceae). Molecules 22 (6):911.
CR  - Ragab OG, Mamdouh D, Bedair R, Smetanska I, Gruda NS et al. (2023). Distinguishing features of Lycium L. species (family Solanaceae) distributed in Egypt based on their anatomical, metabolic, molecular, and ecological characteristics. Frontiers in Plant Science 14: 1162695.
CR  - Trillo C, Toledo BA, Galetto L, Colantonio S (2010). Persistence of the use of medicinal plants in rural communities of the Western Arid Chaco [Córdoba, Argentina]. The Open Complementary Medicine Journal 2: 80-89.
CR  - Wang S, Suh JH, Hung WL, Zheng X, Wang Y (2018). Use of UHPLC-TripleQ with synthetic standards to profile anti-inflammatory hydroxycinnamic acid amides in root barks and leaves of Lycium barbarum. Journal of food and drug analysis 26(2): 572-582.
CR  - Wu W, Bin Hung DK, Chang FW, Ong ET, Chen BH (2012). Anti-inflammatory and anti-angiogenic effects of flavonoids isolated from Lycium barbarum Linnaeus on human umbilical vein endothelial cells. Food Funct 3: 1068-1081.
CR  - Yang Y, An Y, Wang W, Du N, Zhang J, et al. (2017). Nine compounds from the Root Bark of Lycium chinense and their anti-inflammatory activities. Acta pharmaceutica sinica B 7(4): 491-495.
CR  - Yao R (2018).Goji (Fruits of Lycium spp.): Traditional Uses, Quality Assessment, and Value Chain Analysis. Zurich Open Repos Arch
CR  - Yao R, Heinrich M, Weckerle CS (2018). The genus Lycium as food and medicine: A botanical, ethnobotanical and historical review. J. Ethnopharmacol. 212: 50–66.
CR  - Yao X, Peng Y, Xu LJ, Li L, Wu QL et al. (2011) Phytochemical and biological studies of Lycium medicinal plants. Chemistry & Biodiversity 8(6): 976-1010.
CR  - Zhang N, He Z, He S, Jing P (2019). Insights into the importance of dietary chrysanthemum flower (Chrysanthemum morifolium cv. Hangju)-wolfberry (Lycium barbarum fruit) combination in antioxidant and anti-inflammatory properties. Food Res Int 116: 810–818.
UR  - https://doi.org/10.54994/emujpharmsci.1686372
L1  - https://dergipark.org.tr/en/download/article-file/4816771
ER  -