TY  - JOUR
T1  - Investigation of anxiolytic effects of ethanol extract from banana peel (Musa paradisiaca L. var Semeru)
AU  - Soeliono, Ivonne
AU  - Puspitasari, Ika
AU  - Gani, Andayana Puspitasari
AU  - Hadisoewignyo, Lannie
PY  - 2025
DA  - June
JF  - Journal of Research in Pharmacy
JO  - J. Res. Pharm.
PB  - Marmara University
WT  - DergiPark
SN  - 2630-6344
SP  - 351
EP  - 360
VL  - 28
IS  - 1
LA  - en
AB  - Musa paradisiaca L. var Semeru (MPS) is a banana with thick skin that produces unused waste. The potential anxiolytic effect of banana peel ethanol extract (BPE) is explored in this study. There were seven groups in this study, namely the normal control group, CMC Na, alprazolam (0.4 mg/kg), tryptophan (270 mg/kg), 5-HTP (18 mg/kg), BPE (140, 280 mg/kg). The number of rats per group in the light-dark box (LDA) test was 4. Meanwhile, the number of rats per group in the elevated plus maze (EPM) test was 6. BPE (140 and 280 mg/kg) was given to rats one hour before the anxiolytic test using a LDA and EPM. BPE (140 and 280 mg/kg) did not significantly increase entries and time spent in the light chamber of the light-dark box. In addition, it also did not significantly affect entries and time spent in open arms in the elevated plus maze. Referring to the LDA and EPM tests, the ethanol extract of MPS did not significantly reduce anxiety.
KW  - Anxiety
KW  - banana extract
KW  - elevated plus maze
KW  - light-dark activity
CR  - [1]	Stahl SM, Stahl's Essential Psychopharmacology: Neuroscientific Basis and Practical Applications, fifth ed., Cambridge University Press, Cambridge, UK, 2021, pp. 359–378.
CR  - [2]	Mendelson SD, Herbal Treatment of Anxiety: Clinical Studies in Western, Chinese and Ayurvedic Traditions. CRC Press, Boca Raton, FL, USA ,2023.
CR  - [3]	Melton ST, Kirkwood CK. Anxiety Disorders: Generalized Anxiety, Panic, and Social Anxiety Disorders. In: DiPiro JT, Yee GC, Posey LM, Haines ST, Nolin TD, Ellingrod V (Eds.). Pharmacotherapy: A Pathophysiologic Approach. Eleventh ed., McGrawHill, New York, 2020.
CR  - [4]	Lindseth G, Helland B, Caspers J. The effects of dietary tryptophan on affective disorders. Arch Psychiatr Nurs. 2015;29:102–107. https://doi.org/10.1016/j.apnu.2014.11.008.
CR  - [5]	Kikuchi AM, Tanabe A, Iwahori Y. A systematic review of the effect of L-tryptophan supplementation on mood and emotional functioning. J Diet Suppl. 2021;18:316–333. https://doi.org/10.1080/19390211.2020.1746725
CR  - [6]	Young SN. How to increase serotonin in the human brain without drugs. J Psychiatry Neurosci. 2007;32:394–399.
CR  - [7]	Sarfaraz S, Khan M, Bano T, Aftab S, Shakeel SD, Akhtar U, Fatima R. Evaluation of Anti-Depressant Effect of Banana Pulp on Mice. Int J Pharm Sci Clin Pharm. 2020;1:6–9.
CR  - [8]	Gouda TTHMH. Effectiveness of Some Fortified Nutritional Products with Sun Dried Banana Peels on Moody Status of Faculty Education Students in Nujran. J Food Process Technol. 2017;8:8–12. https://doi.org/10.4172/2157-7110.1000705.
CR  - [9]	Samad N, Muneer A, Ullah N, Zaman A, Ayaz MM, Ahmad I. Banana fruit pulp and peel involved in antianxiety and antidepressant effects while invigorate memory performance in male mice: Possible role of potential antioxidants. Pak J Pharm Sci. 2017;30:989–995.
CR  - [10]	File SE, Lippa AS, Beer B, Lippa MT. Animal Tests of Anxiety. Curr Protoc Neurosci. 2004;26. https://doi.org/10.1002/0471142301.ns0803s26.
CR  - [11]	Erigbali P, Kiridi K, Ogregade I. Effects of diets containing unripe plantain diet on brain serotonin in mice. African J Biomed Res. 2017;20:321–323. https://doi.org/10.4314/ajbr.v20i3.
CR  - [12]	Samad N, Khaliq S, Alam M, Yasmin F, Ahmad S, Mustafa S, Azizuddin, Raza U. Tryptophan lessens reserpine induced anxiety, depression and memory impairment by modulating oxidative stress and serotonergic activity. Pak J Pharm Sci. 2021;34:1499–1508. https://doi.org/10.36721/PJPS.2021.34.4.SUP.1499-1508.1.
CR  - [13]	Turner EH, Loftis JM, Blackwell AD. Serotonin a la carte : Supplementation with the serotonin precursor 5-hydroxytryptophan. Pharmacol Ther. 2006;109:325–338. https://doi.org/10.1016/j.pharmthera.2005.06.004.
CR  - [14]	Cohen H, Matar MA, Joseph Z. Animal models of post-traumatic stress disorder. Curr Protoc Neurosci. 2013;1:1–18. https://doi.org/10.1002/0471142301.ns0945s64.
CR  - [15]	Garcia AMB, Martinez RCR, Morato S. Preference for the light compartment of a light/dark cage does not affect rat exploratory behavior in the elevated plus-maze. Psychol Neurosci. 2008;1:73–80. https://doi.org/10.3922/j.psns.2008.1.012.
CR  - [16]	Samad N, Khaliq S, Rehman MHU, Ahmad S, Alam M, Mustafa S, Azizuddin. Exposure to noise augments behavioral deficits in mice: Protective effect of banana peel extract. Pak J Pharm Sci. 2021;34:2333–2340. https://doi.org/10.36721/PJPS.2021.34.6.SUP.2333-2340.1.
CR  - [17]	Samad N, Yasmin F, Khaliq S, Ahmad S, Azizuddin, Mustafa S. Diet supplements of banana fruit pulp mitigates repeated noise stress induced behavioral deficits and oxidative stress. Pak J Pharm Sci. 2020;33:2293–2300. https://doi.org/10.36721/PJPS.2020.33.5.SUP.2293-2300.1.
CR  - [18]	Bowden NA, Sanders JPM, Bruins ME. Solubility of the Proteinogenic α ‑ Amino Acids in Water , Ethanol , and Ethanol − Water Mixtures. J Chem Eng Data. 2018;63:488–497. https://doi.org/10.1021/acs.jced.7b00486.
CR  - [19]	Soeliono I, Puspitasari I, Gani AP, Hadisoewignyo L. Antioxidant Activity and Tryptophan Content of Banana Peel. EnvironmentAsia. 2023;16:99–108. https://doi.org/10.14456/ea.2023.24.
CR  - [20]	Ittiyavirah S, Anurenj D. Adaptogenic studies of acetone extract of Musa paradisiaca L. fruit peels in albino Wistar rats. Int J Nutr Pharmacol Neurol Dis. 2014;4:88. https://doi.org/10.4103/2231-0738.129592.
CR  - [21]	Iqbal N, Afroz S, Mehmood MA, Malik M. Effect of Musa paradisiaca Leaves Ethanol Extract on Memory and Learning Behavior in Mice Model. Pak-Euro J Med Life Sci. 2020;4:133–140. https://doi.org/10.31580/pjmls.v4i3.2333.
CR  - [22]	Hallikeri CS, Joshi SD, Patil VP, Kulkarni VH, Alagawandi KR. Screening of antianxiety activity of unripe fruits of Musa paradisiaca Linn. Indian Drugs. 2012;49:33–35.
CR  - [23]	Reddy AJ, Dubey AK, Handu S, Manocha S. Effects of Musa sapientum stem extract on experimental models of anxiety. Avicenna J Phytomedicine. 2017;7:495–501.
CR  - [24]	Setyarini D, Wasita B. The Effect of Giving Ambon Banana (Musa paradisiaca, Sp) to Decrease of Anxiety Levels in Adult Scizofrenia. Indian J Public Heal Res Dev. 2020;11:1166–1171.
CR  - [25]	Salako OA, Akindele AJ, Balogun AO, Adeyemi OO. Investigation of Antidepressant, Anxiolytic and Sedative Activities of the Aqueous Leaf Extract of Musa sapientum Linn(Banana; Musaceae). Drug Res (Stuttg). 2019;69:136–143. https://doi.org/10.1055/a-0651-7978.
CR  - [26]	Zhang XY, Vollert J, Sena ES, Rice ASC, Soliman N. A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain. BMJ Open Sci. 2021;5:e100135. https://doi.org/10.1136/BMJOS-2020-100135.
CR  - [27]	Ennaceur A. Physiology & Behavior Tests of unconditioned anxiety — Pitfalls and disappointments. Physiol Behav. 2014;135:55–71. https://doi.org/10.1016/j.physbeh.2014.05.032.
CR  - [28]	Ramos A. Animal models of anxiety : do I need multiple tests ? Trends Pharmacol Sci. 2008;29:493–498. https://doi.org/10.1016/j.tips.2008.07.005.
CR  - [29]	Takao K, Miyakawa T. Light/dark Transition Test for Mice. J Vis Exp. 2006:104. https://doi.org/10.3791/104.
UR  - https://dergipark.org.tr/en/pub/jrespharm/issue//1686601
L1  - https://dergipark.org.tr/en/download/article-file/4817666
ER  -