@article{article_1686680, title={Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms}, journal={Journal of Research in Pharmacy}, volume={28}, pages={385–395}, year={2025}, author={Bilgiç Karaağaç, Sinem and Bilgiç Alkaya, Dilek and Ayaz Seyhan, Serap and Öztürk, Buşra Nagihan}, keywords={Diabetes, Pioglitazone hydrochloride, UPLC, Validation, Pharmaceutical Dosage Form}, abstract={The present work reports an ultra-performance liquid chromatography (RP-UPLC) method for the quantitative determination of pioglitazone HCl (PGZ) in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH (50 mm x 2.1 mm, 1.7 μm) column using isocratic elution. The optimized mobile phase consists of phosphate buffer (pH:6) as a solvent-A and 55:45 v/v mixture of acetonitrile as solvent-B. Flow rate was 0.4 ml/min with UV detection at (λmax) 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization (ICH) guidelines with respect to system suitability, specificity, precision, accuracy, linearity.}, number={1}, publisher={Marmara University}