TY  - JOUR
T1  - Investigation of the anticancer effects of some plant seed oils with medicinal uses
AU  - Kurman, Yener
AU  - Dülger, Görkem
AU  - Pasin, Özge
PY  - 2025
DA  - July
JF  - Journal of Research in Pharmacy
JO  - J. Res. Pharm.
PB  - Marmara University
WT  - DergiPark
SN  - 2630-6344
SP  - 1594
EP  - 1604
VL  - 27
IS  - 4
LA  - en
AB  - Cancer is an important health problem and responsible for a significant number of deaths worldwide. Chemotherapy resistance and related side effects prevent cancer treatment from reaching the desired level. In this study, we aimed to investigate the anti-cancer properties of Carthamus tinctorius, Salvia hispanica and Linum usitatissimum seed oils on hepatocellular (HepG2), colorectal (HCT-116) and gastric carcinoma (AGS) cell lines. Human umbilical vein endothelial cells (HUVECs) were used as control cell group. Cell viability of HepG2, HCT-116, AGS and HUVECs was determined by MTT method after treatment with various concentrations of Carthamus tinctorius, Salvia hispanica and Linum usitatissimum. Colorimetric elisa kit was used for quantitative determination of caspase-3 activation. Anti-migratory effect of Carthamus tinctorius and Salvia hispanica were investigated by wound healing assay. Carthamus tinctorius, Salvia hispanica and Linum usitatissimum seed oils significantly inhibited the proliferation of cancer cells. There was an insignificant decrease in the viability of HUVEC cells after seed oils treatments. Migration of HepG2 and AGS cells significantly suppressed by treatment of Salvia hispanica and Carthamus tinctorius respectively. Salvia hispanica and Carthamus tinctorius significantly increased caspase-3 activation in cancer cells. Our results showed that Carthamus tinctorius, Salvia hispanica and Linum usitatissimum exhibited a selective cytotoxicity against HepG2, HCT-116 and AGS cells without damaging normal cells.
KW  - Carthamus tinctorius
KW  - Salvia hispanica
KW  - Linum usitatissimum
KW  - anticancer
KW  - migration
KW  - caspase-3
CR  - [1] Güner A, Kızılşahin S, Nalbantsoy A, Karabay Yavaşoğlu, NÜ. Apoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cells. Biologia. 2020; 75(9), 1465-1471. https://doi.org/10.2478/s11756-020-00458-2
CR  - [2] Güner A, Polatlı E, Akkan T, Bektaş, H, Albay C. Anticancer and antiangiogenesis activities of novel synthesized 2-substituted benzimidazoles molecules. Turkish Journal of Chemistry. 2019; 43(5): 1270-1289. https://doi.org/10.3906/kim-1904-46
CR  - [3] Ma L, Liu L, Ma Y, et al. The Role of E-Cadherin/β-Catenin in Hydroxysafflor Yellow A Inhibiting Adhesion, Invasion, Migration and Lung Metastasis of Hepatoma Cells. Biol Pharm Bull. 2017; 40(10):1706-1715. https://doi.org/10.1248/bpb.b17-00281
CR  - [4] Xu J, Chen Y, Yang R, et al. Cucurbitacin B inhibits gastric cancer progression by suppressing STAT3 activity. Arch Biochem Biophys. 2020; (684):108314. https://doi.org/10.1016/j.abb.2020.108314
CR  - [5] Chang JM, Hung LM, Chyan YJ, Cheng CM, Wu RY. Carthamus tinctorius Enhances the Antitumor Activity of Dendritic Cell Vaccines via Polarization toward Th1 Cytokines and Increase of Cytotoxic T Lymphocytes. Evid Based Complement Alternat Med. 2011; (2011):274858. https://doi.org/10.1093/ecam/nen068
CR  - [6] Loo WT, Cheung MN, Chow LW. The inhibitory effect of a herbal formula comprising ginseng and carthamus tinctorius on breast cancer. Life Sci. 2004; 76(2):191-200. https://doi.org/10.1016/j.lfs.2004.06.021
CR  - [7] Asgarpanah J, Kazemivash N. Phytochemistry, pharmacology and medicinal properties of Carthamus tinctorius L. Chin J Integr Med. 2013; 19(2):153-159. https://doi.org/10.1007/s11655-013-1354-5
CR  - [8] Zhang LL, Tian K, Tang ZH, et al. Phytochemistry and Pharmacology of Carthamus tinctorius L. Am J Chin Med. 2016; 44(2):197-226. https://doi.org/10.1142/S0192415X16500130
CR  - [9] Giulitti F, Petrungaro S, Mandatori S, et al. Anti-tumor Effect of Oleic Acid in Hepatocellular Carcinoma Cell Lines via Autophagy Reduction. Front Cell Dev Biol. 2021; (9):629182. https://doi.org/10.3389/fcell.2021.629182
CR  - [10] Zhu S, Jiao W, Xu Y, et al. Palmitic acid inhibits prostate cancer cell proliferation and metastasis by suppressing the PI3K/Akt pathway. Life Sci. 2021; 286:120046. https://doi.org/10.1016/j.lfs.2021.120046
CR  - [11] Roh JS, Han JY, Kim JH, Hwang JK. Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis. Biol Pharm Bull. 2004; 27(12):1976-1978. https://doi.org/10.1248/bpb.27.1976
CR  - [12] Yasukawa K, Akihisa T, Kasahara Y, et al. Inhibitory effect of alkane-6,8-diols, the components of safflower, on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin. Oncology. 1996; 53(2):133-136. https://doi.org/10.1159/000227549
CR  - [13] Güzel S, Ülger M, Özay Y. Antimicrobial and antiproliferative activities of Chia (Salvia hispanica L.) seeds. International Journal of Secondary Metabolite. 2020; 7(3):174-180. https://doi.org/10.21448/ijsm.722574
CR  - [14] Rosas-Ramírez DG, Fragoso-Serrano M, Escandón-Rivera S, Vargas-Ramírez AL, Reyes-Grajeda JP, Soriano-García M. Resistance-modifying Activity in Vinblastine-resistant Human Breast Cancer Cells by Oligosaccharides Obtained from Mucilage of Chia Seeds (Salvia hispanica). Phytother Res. 2017; 31(6):906-914. https://doi.org/10.1002/ptr.5815
CR  - [15] Sorice A, Guerriero E, Volpe MG, et al. Differential Response of Two Human Breast Cancer Cell Lines to the Phenolic Extract from Flaxseed Oil. Molecules. 2016; 21(3):319. https://doi.org/10.3390/molecules21030319
CR  - [16] Buckner AL, Buckner CA, Montaut S, Lafrenie RM. Treatment with flaxseed oil induces apoptosis in cultured malignant cells. Heliyon. 2019; 5(8):e02251. https://doi.org/10.1016/j.heliyon.2019.e02251
CR  - [17] Arslanoglu SF, Aytac S. The important of flax (Linum usitatissimum L.) in terms of health. International Journal of Life Sciences and Biotechnology. 2020; 3(1):95-107. https://doi.org/10.38001/ijlsb.690295
CR  - [18] Keykhasalar R, Tabrizi MH, Ardalan P, Khatamian N. The Apoptotic, Cytotoxic, and Antiangiogenic Impact of Linum usitatissimum Seed Essential Oil Nanoemulsions on the Human Ovarian Cancer Cell Line A2780. Nutr Cancer. 2021; 73(11-12):2388-2396. https://doi.org/10.1080/01635581.2020.1824001
CR  - [19] Zhou X, Huang N, Chen W, et al. HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways. AMB Express. 2020; 10(1):203. https://doi.org/10.1186/s13568-020-01138-9
CR  - [20] Joseph, Anitha, et al. "Identification of anticancer compounds from Linum usitatissimum seed extract and their effect on HeLa cells." Pharmacognosy Magazine. 2020; 16:69. https://doi.org/10.4103/pm.pm_341_19
CR  - [21] Liang ZE, Yi YJ, Guo YT, Wang RC, Hu QL, Xiong XY. Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum. Mol Med Rep. 2015; 12(5):7629-7636. https://doi.org/10.3892/mmr.2015.4345
CR  - [22] Zhang J, Li J, Song H, Xiong Y, Liu D, Bai X. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. Biomed Pharmacother. 2019; 109:806-814. https://doi.org/10.1016/j.biopha.2018.09.086
CR  - [23] Riboli E, Norat T. Cancer prevention and diet: opportunities in Europe. Public Health Nutr. 2001; 4(2B):475-484. https://doi.org/10.1079/phn2001158
CR  - [24] Ramos S. Effects of dietary flavonoids on apoptotic pathways related to cancer chemoprevention. J Nutr Biochem. 2007; 18(7):427-442. https://doi.org/10.1016/j.jnutbio.2006.11.004
CR  - [25] Park GH, Hong SC, Jeong JB. Anticancer activity of the safflower seeds (Carthamus tinctorius L.) through inducing cyclin D1 proteasomal degradation in human colorectal cancer cells. Korean Journal of Plant Resources. 2016; 29(3): 297-304. https://doi.org/10.7732/kjpr.2016.29.3.297
CR  - [26] Delshad E, Yousefi M, Sasannezhad P, Rakhshandeh H, Ayati Z. Medical uses of Carthamus tinctorius L. (Safflower): a comprehensive review from Traditional Medicine to Modern Medicine. Electron Physician. 2018;10(4):6672-6681. https://doi.org/10.19082/6672
CR  - [27] Xian B, Wang R, Jiang H, et al. Comprehensive review of two groups of flavonoids in Carthamus tinctorius L. Biomed Pharmacother. 2022;(153):113462. https://doi.org/10.1016/j.biopha.2022.113462
CR  - [28] Wu N, Li J, Luo H, Wang D, Bai X. Hydroxysafflor yellow A promotes apoptosis via blocking autophagic flux in liver cancer. Biomed Pharmacother. 2021;(136):111227. https://doi.org/10.1016/j.biopha.2021.111227
CR  - [29] Tang D, Huang T, Tian Q, Wang J. MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells. Drug Des Devel Ther. 2021;(15):1749-1763. https://doi.org/10.2147/DDDT.S288841
CR  - [30] Zhu L, Xue L. Kaempferol Suppresses Proliferation and Induces Cell Cycle Arrest, Apoptosis, and DNA Damage in Breast Cancer Cells. Oncol Res. 2019;27(6):629-634. https://doi.org/10.3727/096504018X15228018559434
CR  - [31] Qiu J, Zhang T, Zhu X, et al. Hyperoside Induces Breast Cancer Cells Apoptosis via ROS-Mediated NF-κB Signaling Pathway. Int J Mol Sci. 2019;21(1):131. https://doi.org/10.3390/ijms21010131
CR  - [32] Chen D, Wu YX, Qiu YB, et al. Hyperoside suppresses hypoxia-induced A549 survival and proliferation through ferrous accumulation via AMPK/HO-1 axis. Phytomedicine. 2020;(67):153138. https://doi.org/10.1016/j.phymed.2019.153138
CR  - [33] Kızılşahin S, Nalbantsoy A, Yavaşoğlu NÜ. In vitro synergistic efficacy of conjugated linoleic acid, oleic acid, safflower oil and taxol cytotoxicity on PC3 cells. Nat Prod Res. 2015;29(4):378-382. https://doi.org/10.1080/14786419.2014.945172
CR  - [34] Xi SY, Zhang Q, Liu CY, Xie H, Yue LF, Gao XM. Effects of hydroxy safflower yellow-A on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice. J Tradit Chin Med. 2012;32(2):243-248. https://doi.org/10.1016/s0254-6272(13)60019-9
CR  - [35] Wang D, Wang S, Liu W, Li M, Zheng Q, Li D. Hydroxysafflor yellow B induces apoptosis via mitochondrial pathway in human gastric cancer cells. Journal of Pharmacy and Pharmacology. 2022; 74(9):1320-1329. https://doi.org/10.1093/jpp/rgac044
CR  - [36] Quintal-Bojórquez NDC, Carrillo-Cocom LM, Hernández-Álvarez AJ, Segura-Campos MR. Anticancer activity of protein fractions from chia (Salvia hispanica L.). J Food Sci. 2021;86(7):2861-2871. https://doi.org/10.1111/1750-3841.15780
CR  - [37] Lim SY. Inhibitory effect of Linum usitatissimum and Perilla frutescens as sources of omega-3 fatty acids on mutagenicity and growth of human cancer cell lines. Journal of Life Science. 2009; 19(12): 1737-1742. https://doi.org/10.5352/JLS.2009.19.12.1737
CR  - [38] Tawfik SA, Awad ET, Abu Bakr HO, Ahmed IM, Ashour E, Gamal-Eldeen AM. Chia seeds oil suppresses the resistance of hepatocellular carcinoma cells to liposomal-doxorubicin and upregulates the tumor suppressor miRNAs. Curr Pharm Biotechnol. 2022; https://doi.org/10.2174/1389201023666220921125258
CR  - [39] Sharula, Wu Z. Regulation of Apoptosis by SYB in HepG2 Liver Cancer Cells is Mediated by the P53/Caspase 9 Axis. Anticancer Agents Med Chem. 2017;17(7):941-947. https://doi.org/10.2174/1871520617666170327161433
CR  - [40] Goyal A, Sharma V, Upadhyay N, Gill S, Sihag M. Flax and flaxseed oil: an ancient medicine & modern functional food. J Food Sci Technol. 2014;51(9):1633-1653. https://doi.org/10.1007/s13197-013-1247-9
CR  - [41] Deshpande R, Raina P, Shinde K, Mansara P, Karandikar M, Kaul-Ghanekar R. Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer. Prostaglandins Other Lipid Mediat. 2019;143:106332. https://doi.org/10.1016/j.prostaglandins.2019.04.002
CR  - [42] Guerriero E, Sorice A, Capone F, et al. Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines. Int J Oncol. 2017;50(2):468-476. https://doi.org/10.3892/ijo.2017.3835
CR  - [43] Truan JS, Chen JM, Thompson LU. Comparative effects of sesame seed lignan and flaxseed lignan in reducing the growth of human breast tumors (MCF-7) at high levels of circulating estrogen in athymic mice. Nutr Cancer. 2012;64(1):65-71. https://doi.org/10.1080/01635581.2012.630165
CR  - [44] Baud S, Lepiniec L. Physiological and developmental regulation of seed oil production. Prog Lipid Res. 2010;49(3):235-249. https://doi.org/10.1016/j.plipres.2010.01.001
CR  - [45] Liang ZE, Yi YJ, Guo YT, Wang RC, Hu QL, Xiong XY. Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum. Mol Med Rep. 2015;12(5):7629-7636. https://doi.org/10.3892/mmr.2015.4345
CR  - [46] Lehraiki A, Attoumbré J, Bienaimé C, et al. Extraction of lignans from flaxseed and evaluation of their biological effects on breast cancer MCF-7 and MDA-MB-231 cell lines. J Med Food. 2010;13(4):834-841. https://doi.org/10.1089/jmf.2009.0172
CR  - [47] Shaer N, Al-Abbas N. Potential effect of Chia Seeds Crude Extract Nanoparticles on Mcf-7 Breast Cancer Cell Line. Al-Azhar International Medical Journal. 2022; 3(2): 123-127. https://doi.org/10.21608/AIMJ.2022.97818.1585
CR  - [48] Chen X, Wang Y, Zhang L, Gao Y. Hydroxysafflor Yellow A of Carthamus Tinctorius L., Represses the Malignant Development of Esophageal Cancer Cells via Regulating NF-κB Signaling Pathway. Cell Biochem Biophys. 2020;78(4):511-520. https://doi.org/10.1007/s12013-020-00934-1
UR  - https://dergipark.org.tr/en/pub/jrespharm/issue//1690999
L1  - https://dergipark.org.tr/en/download/article-file/4835116
ER  -